Chinese Journal OF Rice Science >
Function and Effect Analysis of a New Gene Wx410 Regulating Amylose Synthesis in Rice
# These authors contributed equally to this work
Received date: 2022-01-06
Revised date: 2022-03-14
Online published: 2022-11-10
【Objective】 Mining new allelic variants of the Wx gene and identifying the effect of the new Wx410 allele on rice quality traits. 【Method】 Using Wxlv,Wxa and Wxb alleles as templates, the A-G point mutation at base 101 of exon 10 was performed by PCR, and Wx410 sentinel mutation plant expression vectors in different Wx allelic backgrounds (pEGFC-Wxlv410, pEGFC-Wxa410 and pEGFC-Wxb410) were constructed respectively, and the genetic effect of the variation at this locus on rice quality was analyzed by transforming the glutinous rice variety Suyunuo. 【Results】 The expression level of the Wx gene in the endosperm of the transgenic plants pEGFC-Wxlv410, pEGFC-Wxa410 and pEGFC-Wxb410 7 and 14 days after flowering was not significantly different from that of the positive control, while the activity of GBSS Ⅰ was highly significantly lower than that of the positive control. The transgenic plants had significantly lower amylose content in mature seeds compared to the wild-type. pEGFC-Wxa410 and pEGFC-Wxb410 had significantly higher gelatinous consistency and pEGFC-Wxlv410 had significantly lower gelatinous consistency of endosperm compared to the positive control. 【Conclusion】 Wx410 is a new functional allele for starch synthesis in rice. The allele explains 4%−6% of amylose content variation, which just fills the gap in the range of amylose content regulated by the currently identified complex alleles, providing a richer genetic resource for rice taste and processing-related quality improvement.
Key words： rice; Wx gene; amylose content
MAO Hui, PENG Yan, MAO Bigang, SHAO Ye, ZHENG Wenjie, HU Liming, ZHOU Kai, ZHAO Bingran . Function and Effect Analysis of a New Gene Wx410 Regulating Amylose Synthesis in Rice[J]. Chinese Journal OF Rice Science, 2022 , 36(6) : 579 -585 . DOI: 10.16819/j.1001-7216.2022.220103
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