Chinese Journal OF Rice Science >
Fine Mapping of qGL1.1, a Minor QTL for Grain Length, Using Near Isogenic Lines Derived from Residual Heterozygotes in Rice
Received date: 2019-11-21
Revised date: 2019-12-28
Online published: 2020-03-10
【Objective】The objective of this study is to fine-map qTGW1.1b which was previously mapped in a 521.8-kb region on the long arm of rice chromosome 1.【Method】In this study, two BC2F9 plants carrying qTGW1.1 and qTGW1.2, respectively, was crossed and produced an F4 population. Three plants carrying sequential heterozygous segments in the interval Wn28826-RM1231 were selected and selfed to develop three sets of near isogenic lines (NILs) comprising the two homozygous genotypes. The NILs in F5:6 were planted in Hangzhou in 2017 and the 1000-grain weight, grain length and grain width were measured. The effect of qTGW1.1b was validated by two-way analysis of variance (ANOVA) using SAS program. Then six new sequential residual heterozygotes carrying smaller heterozygous segments overlapped in the interval Wn28826-RM1231 were identified. Six sets of pairwise NILs in F8:9 were planted in Lingshui in 2018 and the three traits were measured. Phenotypic differences between the two homozygous genotypic groups were analyzed using ANOVA.【Results】The allelic direction of qTGW1.1b remained consistent and the genetic effects were stable in the two trials. The Milyang 46 allele could significantly increase grain length and 1000-grain weight by 0.027 mm and 0.17 g with the contribution up to 27.12% and 19.09%, respectively.【Conclusion】Considering that qTGW1.1b stably showed significant effect to grain length but not to grain width in both previous and this study, qTGW1.1b was renamed as qGL1.1. As a result, qGL1.1 was delimited into a 76.8-kb region franked by Wn29077 and Wn29154 based on comparing of the segregating regions among the six sets of NILs.
Key words： rice; near isogenic line; minor effect; grain length; fine mapping
Panpan LI, Yujun ZHU, Liang GUO, Jieyun ZHUANG, Yeyang FAN . Fine Mapping of qGL1.1, a Minor QTL for Grain Length, Using Near Isogenic Lines Derived from Residual Heterozygotes in Rice[J]. Chinese Journal OF Rice Science, 2020 , 34(2) : 125 -134 . DOI: 10.16819/j.1001-7216.2020.9125
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