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Identification of Strain 7-5, Antagonistic to Rice Sheath Blight, and Preliminary Study of Its Biocontrol Mechanism
Received date: 2017-06-19
Revised date: 2017-09-18
Online published: 2018-05-10
【Objective】The objective is to obtain antagonistic bacteria for biocontrol on rice sheath blight and reveal the biological control mechanism.【Method】The control experiment of rice sheath blight by seven antagonistic bacteria stored in our lab was performed using pot cultures at the seedling stage. The strain 7-5 was identified based on a 16S rDNA BLAST and the strain’s physiological and biochemical characterization. The antifungal compounds produced by bacterial strain 7-5 were detected by PCR amplification and thin layer chromatography (TLC). 【Result】The suppressive effects of bacteria 7-5, 4-74 and 4-78 on the rice sheath blight in a greenhouse were as high as 69.0%, 70.8% and 75.4%, respectively. Strain 7-5 could strongly inhibit the growth of Rhizoctoniasolani, Moniliniafructicola and Botrytis cinerea, whereas both strains 4-74 and 4-78 had narrow inhibitory spectra. Strain 7-5 was identified as Pseudomonas chlororaphis and produced many antifungal factors, including phenazine-1-carboxylicacid, 2,4-diacetylphloroglucinol, pyoluteorin, siderophores, hydrogen cyanide and extracellular proteinase, but not including pyrrolnitrin, chitinase and cellulase.【Conclusion】The production of antibiotics plays a key role for P. chlororaphis7-5 to control the rice disease.
Qingxia ZHANG, Ying ZHANG, Lingling HE, Xijun CHEN, , Zhaolin JI . Identification of Strain 7-5, Antagonistic to Rice Sheath Blight, and Preliminary Study of Its Biocontrol Mechanism[J]. Chinese Journal OF Rice Science, 2018 , 32(3) : 277 -284 . DOI: 10.16819/j.1001-7216.2018.7074
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