Chinese Journal OF Rice Science ›› 2018, Vol. 32 ›› Issue (3): 277-284.DOI: 10.16819/j.1001-7216.2018.7074
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Qingxia ZHANG, Ying ZHANG, Lingling HE, Xijun CHEN*(), , Zhaolin JI
Received:
2017-06-19
Revised:
2017-09-18
Online:
2018-05-10
Published:
2018-05-10
Contact:
Xijun CHEN
张清霞, 张迎, 何玲玲, 陈夕军*(), 童蕴慧, 纪兆林
通讯作者:
陈夕军
基金资助:
CLC Number:
Qingxia ZHANG, Ying ZHANG, Lingling HE, Xijun CHEN, , Zhaolin JI. Identification of Strain 7-5, Antagonistic to Rice Sheath Blight, and Preliminary Study of Its Biocontrol Mechanism[J]. Chinese Journal OF Rice Science, 2018, 32(3): 277-284.
张清霞, 张迎, 何玲玲, 陈夕军, 童蕴慧, 纪兆林. 水稻纹枯病拮抗细菌7-5的鉴定及其生防机制初步研究[J]. 中国水稻科学, 2018, 32(3): 277-284.
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URL: http://www.ricesci.cn/EN/10.16819/j.1001-7216.2018.7074
引物 Primer | 序列 Sequence (5’-3’) | 基因 Amplified gene | 片段大小 Amplified segment/bp | 文献 Reference |
---|---|---|---|---|
16S-63F | CAGGCCTAACACATGCAAGTC | 16SrDNA | 1500 | [19] |
16S-1387R | GGGCGGTGATGTACAAGGC | |||
phl2a | GAGGACGTCGAAGACCACCA | phlD | 745 | [20] |
phl2b | ACCGCAGCATCGTGTATGAG | |||
PCA2a | TGCCAAGCCTCGCTCCAAC | phzCD | 1400 | [20] |
PCA3b | CCGCGTTGTTCCTCGTTCAT | |||
pltcf | GAAGCCGAAGGCATGGACCC | pltC | 450 | 本研究This study |
pltcr | GCCGCGTCGAAGGTCTTGCA | |||
prnCF | CCACAAGCCCGGCCAGGAGC | prnC | 720 | [21] |
prnCR | GAGAAGAGCGGGTCGATGAAGCC |
Table 1 Primers used in this study.
引物 Primer | 序列 Sequence (5’-3’) | 基因 Amplified gene | 片段大小 Amplified segment/bp | 文献 Reference |
---|---|---|---|---|
16S-63F | CAGGCCTAACACATGCAAGTC | 16SrDNA | 1500 | [19] |
16S-1387R | GGGCGGTGATGTACAAGGC | |||
phl2a | GAGGACGTCGAAGACCACCA | phlD | 745 | [20] |
phl2b | ACCGCAGCATCGTGTATGAG | |||
PCA2a | TGCCAAGCCTCGCTCCAAC | phzCD | 1400 | [20] |
PCA3b | CCGCGTTGTTCCTCGTTCAT | |||
pltcf | GAAGCCGAAGGCATGGACCC | pltC | 450 | 本研究This study |
pltcr | GCCGCGTCGAAGGTCTTGCA | |||
prnCF | CCACAAGCCCGGCCAGGAGC | prnC | 720 | [21] |
prnCR | GAGAAGAGCGGGTCGATGAAGCC |
Fig.4. PCR amplification of antibiotics gene from bacterial strain 7-5. M,2000 bp marker;1–3,PRN amplification productsof FD6, 7-5 and 2P24;4–6,PCAamplification products of PAO17-5 and FD6;7–9,PLT amplification productsof FD6, 7-5 and 2P24;10–12,PHL amplification productsof FD6, 7-5 and L5.
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