Chinese Journal OF Rice Science >
Identification and Gene Mapping of a Spotted-leaf Mutant hm197 in Rice
*Corresponding author：E-mailbeishangd@163.com
Received date: 2015-03-12
Revised date: 2015-03-24
Online published: 2015-09-10
A stable inherited rice spotted-leaf mutant hm197 was isolated from a diepoxybutane-induced IR64 mutant bank. Under natural conditions, brown lesions were first observed on the tips of the mutant leaves 10 weeks after sowing, and spread gradually downward to cover the whole leaf blades. Genetic analysis indicated that the mutant trait was controlled by a novel single recessive nuclear gene, tentatively termed as splhm97,located in the long arm of chromosome 4. Agronomic traits including the plant height, seed-setting rate and 1000-grain weight were significantly decreased in hm197. The initiation of brown lesions in hm197 was induced by natural sun light. In addition, the photosynthetic pigment contents and net photosynthetic rate in the mutant were significantly lower than those in the wild type IR64. Histochemical analysis showed that H2O2 and accumulation were presented in and around the lesions in hm197. Furthermore, senescence-related parameters including the activities of SOD, APX,total soluble protein and MDA contents were also significantly altered in the mutant while resistance to bacterial blight pathogens was largely enhanced in the mutant in contrast to the wild type IR64.
Xiao-hong LI, Yong-feng SHI, Xiao-bo ZHANG, Bao-hua FENG, Li-xin SONG, Hui-mei WANG, Xia XU, Qi-na HUANG, Dan GUO, Jian-li WU . Identification and Gene Mapping of a Spotted-leaf Mutant hm197 in Rice[J]. Chinese Journal OF Rice Science, 2015 , 29(5) : 447 -456 . DOI: 10.3969/j.issn.1001G7216.2015.05.001
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