Chinese Journal OF Rice Science

Chinese Journal OF Rice Science >

2015 , Vol. 29 >Issue 1: 97 - 105

DOI: https://doi.org/10.3969/j.issn.1001-7216.2015.01.012

Orginal Article

Improvement of the Micro-chamber Inoculation Method for Determination of Rice Seedling Resistance to Sheath Blight (Rhizoctonia solani)

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  • 1College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321004, China
    2Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
    3Key Laboratory of Crop Genetics and Physiology of Jiangsu Province, College of Agriculture, Yangzhou University/Key Laboratory of Plant Functional Genomics of the Ministry of Education, Yangzhou 225009, China
    4Hunan Provincial Key Laboratory of Crop Germplasm Innovation and Utilization and College of Agronomy, Hunan Agricultural University, Changsha 410128, China
*Corresponding author, E-mail:squ@mail.zaas.ac.cn

Received date: 2014-03-02

  Revised date: 2014-04-13

  Online published: 2015-01-10

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Abstract

The sheath blight resistance in rice belongs to a typical quantitative trait, and most of the rice cultivars show low levels of resistance to the disease with small differences. The inoculation and scoring system for rice sheath blight is an important basis for breeding resistant rice cultivars. The micro-chamber method for determination of rice responses to the sheath blight pathogen Rhizoctonia solani was improved by inoculation to rice seedlings with similar growth vigour under controlled illumination, temperature and humidity conditions in a plant growth chamber. The rice cultivars YSBR1, Teqing, Taijing 394, Nipponbare and Lemont showed high to low levels of sheath blight resistance , which was consistent with the results of field inoculation in adult rice plants. RT-PCR analysis showed that the expression of four rice genes related to disease resistance was induced in inoculated seedlings and adult plants. Based on the height of the pulvinus of the second leaf from the top and the straightened seedling height the sheath blight scores ranged from 2.82 to 8.54 and from 1.20 to 3.39, respectively. The scoring method based on the height of the pulvinus led to similar disease scores for both seedlings and adult plants, and thus is more suitable for the micro-chamber inoculation system.

Key words： rice; sheath blight; Rhizoctonia solani; micro-chamber screening method; disease resistance-related genes

Cite this article

Guo-juan XU, Zheng-jie YUAN, Shi-min ZUO, Xue-biao PAN, Zhi-long WANG, Shao-hong QU . Improvement of the Micro-chamber Inoculation Method for Determination of Rice Seedling Resistance to Sheath Blight (Rhizoctonia solani)[J]. Chinese Journal OF Rice Science, 2015 , 29(1) : 97 -105 . DOI: 10.3969/j.issn.1001-7216.2015.01.012

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