Chinese Journal OF Rice Science ›› 2018, Vol. 1 ›› Issue (1): 137-145.DOI: 10.16819/j.1001-7216.2018.7091
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Tao CHEN1,2, Shanlei ZHANG1, Ling ZHAO1, Yadong ZHANG1, Zhen ZHU1, Qingyong ZHAO1, Lihui ZHOU1, Shu YAO1, Chunfang ZHAO1, Wenhua LIANG1, Cailin WANG1,*()
Received:
2017-08-01
Online:
2018-01-10
Published:
2018-03-10
Contact:
Cailin WANG
陈涛1,2, 张善磊1, 赵凌1, 张亚东1, 朱镇1, 赵庆勇1, 周丽慧1, 姚姝1, 赵春芳1, 梁文化1, 王才林1,*()
通讯作者:
王才林
基金资助:
CLC Number:
Tao CHEN, Shanlei ZHANG, Ling ZHAO, Yadong ZHANG, Zhen ZHU, Qingyong ZHAO, Lihui ZHOU, Shu YAO, Chunfang ZHAO, Wenhua LIANG, Cailin WANG. Development and Verification of a Functional Marker Associated with Resistance to ALS Inhibitor Herbicide[J]. Chinese Journal OF Rice Science, 2018, 1(1): 137-145.
陈涛, 张善磊, 赵凌, 张亚东, 朱镇, 赵庆勇, 周丽慧, 姚姝, 赵春芳, 梁文化, 王才林. ALS抑制剂类除草剂抗性水稻功能标记的开发与验证[J]. 中国水稻科学, 2018, 1(1): 137-145.
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URL: http://www.ricesci.cn/EN/10.16819/j.1001-7216.2018.7091
引物名称 Primer | 引物序列(5′-3′) Sequence (5′-3′) |
---|---|
ALS-O-F | AGGTGAGGCAATCATCGCTAC |
ALS-O-R | ATGGGTCTATTCAGGTCAAACA |
ALS-I-F | AACCAACATTTGGGTATGGTTGTGCTAA |
ALS-I-R | CTATTTGCCTTGTAAAACCTATCCTTCC |
Table 1 Sequences of primer to detect the base variation by tetra-primer ARMS-PCR system in rice.
引物名称 Primer | 引物序列(5′-3′) Sequence (5′-3′) |
---|---|
ALS-O-F | AGGTGAGGCAATCATCGCTAC |
ALS-O-R | ATGGGTCTATTCAGGTCAAACA |
ALS-I-F | AACCAACATTTGGGTATGGTTGTGCTAA |
ALS-I-R | CTATTTGCCTTGTAAAACCTATCCTTCC |
Fig. 1. Strategy for Tetra-primer ARMS-PCR system to detect the two continuous base mutationsfor ALS gene in rice. The exon,non-coding regionsand primers are showed in black, blue and red bases, respectively. The differential bases in sequences are in shadow and the key bases involving in the resistance to ALS inhibitor herbicides are in italic and shadow.
Fig. 2. Electrophoresis detection of ALS genotypes by Tetra-primer ARMS-PCR in different rice varieties or lines. M, DNA marker (DL2000); 1, Mutant of Huanghuazhan M-1; 2, Huanghuazhan; 3, Nanjing 2728; 4, Huaidao5; 5, Zhendao 99.
Fig. 4. Electrophoresis detection of ALS genotype by Tetra-primer ARMS-PCR in F2 population derived from Huaidao 5/Huanghuazhan M-1. M,DNA Marker (DL2000); 1, Huanghuazhan M-1; 2, Huaidao5; 3, F1 (Huaidao5/ Huanghuazhan M-1); 4-17, Parts of plants from F2 population.
Fig.5. Resistance test of F2 population derived from Huaidao5/Huanghuazhan M-1 to ALS inhibitor herbicideimazethapyr. A, Plants without homozygous genotype containing mutant ALS gene; B, Plants with homozygous genotype containing mutant ALS gene; C, Plants with heterozygous genotype containing mutant ALS gene.
[1] | 中国农村技术开发中心. 我国水稻机械化直播技术体系日臻完善. 中国农业科技导报, 2017, 19(2): 139. |
China Rural Technology Development Center.More and more perfect technology system of mechanized direct-sowing rice in China.J AgricSciTechnol, 2017, 19(2): 139. (in Chinese) | |
[2] | 张云月, 卢宗志, 李洪鑫, 崔海兰. 杂草对乙酰乳酸合成酶抑制剂类除草剂抗药性的研究进展. 杂草科学, 2013, 31(2): 1-5. |
Zhang Y Y, Lu Z Z, Li H X, Cui H N.Research progress on weed resistance to acetolactate synthase inhibitors.Weed Sci, 2013, 31(2):1-5.(in Chinesewith English abstract) | |
[3] | Duggleby R G, McCourt J A, Guddat L W. Structure and mechanism of inhibition of plant acetohydroxyacid synthase.Plant PhysiolBiochem, 2008, 46: 309-324. |
[4] | Sudianto E, Beng-Kah S, Ting-Xiang N, Saldain N E, Scott R C, Burgos N R.Clearfieldrice: Its development, success, and key challenges on a global perspective.Crop Prot, 2013, 49: 40-51. |
[5] | WenefridaI, Utomo HS, Meche MM, Nash JL. Inheritance of herbicideresistance in two germplasm lines of Clearfield®rice(Oryza sativa L.). CanJPlant Sci,2007, 87: 659-669. |
[6] | GoulartICGR, Matzenbacher FO, Merotto A JR.Differential germination pattern of rice cultivars resistant to imidazolinone herbicides carrying different acetolactate synthase gene mutations.Weed Res, 2012, 52: 224-232. |
[7] | 陈竹锋, 王承旭, 柳威, 唐晓艳, 邓兴旺.水稻抗除草剂蛋白及其在植物育种中的应用: CN201210037789.9.2013-04-17. |
Chen Z F, Wang C X, Liu W, Tang X X, Deng X W. Rice herbicide resistant protein and application thereof in plant breeding[P]: CN201210037789.9.2013-04-17. (in Chinese) | |
[8] | 陈涛, 骆名瑞, 张亚东, 朱镇, 赵凌, 赵庆勇, 周丽慧, 姚姝, 于新, 王才林. 利用四引物扩增受阻突变体系PCR技术检测水稻低直链淀粉含量基因Wx-mq. 中国水稻科学, 2013, 27(5): 529-534. |
Chen T, Luo M R, Zhang Y D, Zhu Z, Zhao Q Y, Zhou L H, Yao S, Yu X, Wang C L.Detection of Wx-mqgene for low-amylose content by tetra-primer amplification refractory mutation system PCR in rice. Chin J Rice Sci, 2013, 27(5): 529-534. (in Chinesewith English abstract) | |
[9] | 李军, 李白, 高荣村. 利用四引物扩增受阻突变体系PCR技术检测水稻光温敏核不育基因p/tms12-1. 中国水稻科学, 2014, 28(4): 442-446. |
Li J, Li B, Gao R C.Detection of gene p/tms12-1 for photoperiod-and thermo-sensitive genic male sterility by tetra-primer amplicationrefractory mutation system PCR in rice.Chin J Rice Sci, 2014, 28(4): 442-446.(in Chinesewith English abstract) | |
[10] | YeS,DhillonS,Ke X Y, Collins AR, Day I N M. Anefficientprocedureforgenotypingsinglenucleotidepolymorphisms.Nucleic Acids Res, 2001, 29, e88. |
[11] | Lalitha S.Primer Premier 5.Biotech Software & Internet Report, 2000, 1(6): 270-272. |
[12] | Murray MG, Thompson WF.Rapid isolation of highmolecular-weightplant DNA.Nucleic Acids Res, 1980, 8: 4321-4325. |
[13] | Chen X, Temnykh S, Xu Y, Cho Y G, McCouch S R. Development of a microsatellite framework map providing genome-wide coverage in rice (Oryza sativa L.). TheorAppl Genet, 1997, 95: 553-567. |
[14] | 宋贵生, 冯德江, 魏晓丽, 唐家斌, 朱桢. 水稻乙酰乳酸合成酶基因的克隆和功能分析. 中国农业科技导报, 2007, 9(3): 66-72. |
Song G S, Feng D J, Wei X L, Tang J B, Zhu Z.Isolation and functional analysis of rice acetolactate-synthase (ALS).J AgricSci Tech China,2007,9(3): 66-72.(in Chinesewith English abstract) | |
[15] | Boutsalis P, Karotam J, Powles S B.Molecular basis of resistance to acetolactatesynthase-inhibiting herbicides inSisymbriumorientale and Brassica tournefortii.PesticSci, 1999, 55: 507-516. |
[16] | Tan S, Evans R, Singh B.Herbicidal inhibitors of amino acid biosynthesis and herbicide tolerant crops. Amino Acids, 2006, 30:195-204. |
[17] | Zhou Q Y, Liu W P, Zhang Y S, Liu K K.Action mechanisms of acetolactate synthase-inhibiting herbicides.PesticBiochem Phys, 2007, 89: 89-96. |
[18] | Massa D, Krenz B, Gerhards R.Target-site resistance to ALS-inhibiting herbicides inAperaspica-venti populations is conferred by documented and previously unknown mutations. Weed Res, 2011, 51: 294-303. |
[19] | 张保龙, 王金彦, 陈天子, 凌溪铁.粳稻的ALS突变型基因及其蛋白和应用: CN201710120061.5.2017-03-02. |
Zhang B L, Wang J Y, Chen T Z, Ling X T. Mutant ALS gene and its applications and japonica[P]: CN201710120061.5 .2017-03-02. (in Chinese) | |
[20] | Mallory-Smith C A, Thill D C, Dial M J. Identification of sulfonylurea herbicide-resistant Prickly Lettuce (Lactucaserriola). Weed Technol, 1990, 4: 163-168. |
[21] | 任洪雷. 乙酰乳酸合成酶及ALS基因研究概述. 中国农学通报, 2016, 32(26): 37-42. |
Ren H L.Acetolactatesynthase andALSgene research.Chin AgricBul, 2016,32(26): 37-42. (in Chinesewith English abstract) | |
[22] | Délye C.Unravelling the genetic bases ofnon-target-site-based resistance (NTSR)to herbicides: A major challenge for weedscience in the forthcoming decade.Pest ManagSci, 2013, 69: 176-187. |
[23] | Délye C, Jasieniuk M, Le Corre V.Deciphering the evolution of herbicide resistance in weeds.Trends Genet, 2013, 29: 649-658. |
[24] | Rey-CaballeroJ, MenéndezJ, Osuna M D, Salas M, Torra J.Target-site and non-target-site resistance mechanisms to ALS inhibiting herbicides in Papaver rhoeas. PesticBiochemPhysiol, 2017, 138: 57-65. |
[25] | Okuzaki A, Shimizu T, Kaku K, Kawai K, Toriyama K.A novel mutated acetolactate synthase gene conferring specificresistance to pyrimidinylcarboxy herbicides in rice.Plant MolBiol, 2007, 64: 219-224. |
[26] | Yu Q, Powles S B.Resistance to AHAS inhibitor herbicides:current understanding.Pest ManagSci, 2014, 70: 1340-1350. |
[27] | Guttieri M J, Eberlein C V, Thill D C.Diverse mutations in the acetolactate synthase gene conferchlorsulfuron resistance in kochia (Kochia scoparia) biotypes.Weed Sci, 1995, 43: 175-178. |
[28] | Sibony M, Michel A, Haas H U, Rubin B,Hurle K.Sulfometuron-resistant Amaranthusretroflexus: Cross-resistance and molecular basis for resistance to acetolactate synthase-inhibiting herbicides. Weed Res, 2001, 41: 509-522. |
[29] | Tranel P J, Wright T R.Resistance of weeds to ALS-inhibiting herbicides: Whathave we learned?Weed Sci, 2002, 50:700-712. |
[30] | Kadaru S, Zhang W Q, Yadav A,OardJH. Development and application of allele-specific PCR assaysfor imazethapyr resistance in rice (Oryza sativa). Euphytica,2008, 160:431-438. |
[31] | Roso AC, MerottoAJr,Delatorrea CA, MenezesbVG.Regional scale distribution of imidazolinone herbicide-resistant alleles in red rice (Oryza sativa L.) determined through SNP markers. Field Crops Res, 2010, 119: 175-182. |
[32] | Rosas J E, Bonnecarrère V, Vida F P D. One-step, codominant detection of imidazolinone resistance mutations in weedy rice (Oryza sativa L.). Electronic J Biotechnol, 2014, 17: 95-101. |
[33] | Burgos NR,Norsworthy JK, Scott RC, Smith KL.Red rice (Oryza sativa) status after 5 years of imidazolinone-resistant rice technology in Arkansas. Weed Technol, 2008, 22:200-208. |
[34] | BusconiM,Rossi D, Lorenzoni C, Baldi G, Fogher C. Spread of herbicide-resistant weedy rice (red rice,Oryza sativa L.) after 5 years of Clearfield rice cultivation in Italy. Plant Biol, 2012, 14: 751-759. |
[35] | Heap I.International survey of herbicide resistant weeds.[2017-07-01].. |
[36] | 黄春艳, 陈铁保, 王宇, 孙宝宏, 杨绍义. 咪唑啉酮类除草剂对后茬作物安全性研究初报. 农药学学报, 2001, 3(2): 29-34. |
Huang C Y, Chen T B, Wang Y, Sun B H, Yang S Y.Study on safety of imidazolinoneherbicides to succeeding crops.Chin J PesticSci, 2001, 3(2): 29-34.(in Chinesewith English abstract) | |
[37] | Tan S, Evans RR, Dahmer ML, Singh BK, Shaner DL.Imidazolinone-tolerant crops: History, current status and future.Pest ManagSci, 2005, 61:246-257. |
[38] | Dauer J,Hulting A, Carlson D,Mankin L,Harden J, Mallory-Smith C.Gene flow from single and stacked herbicide-resistant rice (Oryza sativa): Modeling occurrence of multiple herbicide-resistant weedy rice. Pest ManagSci, 2017, 74(2):348-355 |
[39] | Green J M, Owen M D K.Herbicide-resistant crops: utilities and limitations for herbicide-resistant weed management.J Agric Food Chem, 2011, 59: 5819-5829. |
[40] | 仲义, 王云鹏, 代秀云, 刘文国. 利用玉米萌动胚获得抗除草剂转基因新材料. 湖北农业科学, 2014, 53(17):4001-4004. |
Zhong Y, Wang Y P, Dai X X, Liu W G.Generating herbicide resistant transgenic plants in maize with germinating embryo as explants.Hubei AgricSci, 2014, 53(17): 4001-4004.(in Chinese) | |
[41] | 胡茂龙, 浦惠明, 龙卫华, 高建芹, 戚存扣, 张洁夫, 陈松. 油菜乙酰乳酸合酶突变体S638N的酶学特性及其对ALS类除草剂的抗性. 作物学报, 2015, 41(9): 1353-1360. |
Hu M L, Pu H M, Long W H, Gao J Q, Qi C K, Zhang J F, Chen S.Enzymatic characteristics of acetolactatesyn- thasemutant S638N in Brassica napus and its resistance to ALS inhibitor herbicides. ActaAgron Sin, 2015, 41(9): 1353-1360. (in Chinesewith English abstract) | |
[42] | 连丽君, 李莹, 王娟, 郭宁, 张可炜. 转betA/als基因棉花生存竞争力和基因漂流的调查. 山东大学学报:理学版, 2009, 44(5): 20-27. |
Lian L J, Li Y, Wang J, Guo N, Zhang K W.Investigations on survival competitiveness and gene flow of transgenic cotton withbeta/als gene in the field. J Shandong Univ:Nat Sci, 2009, 44(5): 20-27. (in Chinesewith English abstract) | |
[43] | 师志刚, 夏雪岩, 刘正理, 程汝宏. 谷子抗咪唑乙烟酸新种质的初步研究. 河北农业科学, 2010, 14(11): 133-134, 136. |
Shi Z G, Xia X Y, Liu Z L, Cheng R H.Preliminary study on new imazethapyrresistant foxtail millet germplasm. J Hebei AgricSci, 2010, 14(11): 133-134,136. (in Chinese) |
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