Mapping of QTLs for Grain Shape Using WholeGenome Resequenced  Chromosome Segment Substitution Lines in Rice

doi:10.3969/j.issn.1001-7216.2011.04.004

Chinese Journal of Rice Science ›› 2011, Vol. 25 ›› Issue (4): 365-369.DOI: 10.3969/j.issn.1001-7216.2011.04.004

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Mapping of QTLs for Grain Shape Using WholeGenome Resequenced Chromosome Segment Substitution Lines in Rice

XU Jian-Jun^{1, 2} , ZHAO Qiang³, TANG Zai-Xiang¹, ZHAO Yuan-Feng¹, ZHU Lei¹, XU Chen-Wu¹, GU Ming-Hong¹, HAN Bin³

¹Jiangsu Key Laboratory of Crop Genetics and Physiology /Key Laboratory of Plant Function Genomics, Ministry of Education, Yangzhou University, Yangzhou 225009, China; ²Songjiang Agricultural Technology and Popularization Center of Shanghai, Shanghai 201613, China; ³Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai 200233, China

Received:2010-10-29 Revised:2010-11-30 Online:2011-07-10 Published:2011-07-10

利用重测序的染色体片段代换系群体定位水稻粒型QTL

徐建军^{1, 2}, 赵强³, 汤在祥¹, 赵元凤¹, 朱磊¹, 徐辰武¹, 顾铭洪¹, 韩斌³

¹扬州大学教育部植物功能基因组学重点实验室/江苏省作物遗传生理国家重点实验室，江苏扬州 225009； ²上海市松江区农业技术推广中心，上海 201613； ³中国科学院植物生理生态研究所，上海 200233

基金资助:
国家973计划资助项目（2005CB120807）

Abstract

Abstract: One hundred and twentyeight chromosome segment substitution lines derived from 9311 as recipient and Nipponbare as donor, were used for mapping QTLs for grain shape by combining the sequencingbased Binmap with a multiple linear regression analysis. Six and two QTLs were identified for grian length(GL) and grian width(GW), respectively. qGL3.1 was mapped in a region of 5 792 954 bp on chromosome 3, qGL3.2 in a region of 917 878 bp on chromosome 3; qGL81 in a region of 889 543 bp on chromosome 8; qGL8.2 in a region of 208 614 bp on chromosome 8; qGL9.1 in a region of 1 149 685 bp on chromosome 9; qGL11.1 in a region of 3 184 760 bp on chromosome 11; qGW1.1 in a region of 200 070 bp on chromosome 1; and qGW5.1 in a region of 704 905 bp on chromosome 5. The results provided a foundation for fine mapping QTLs and subsequent gene cloning.

Key words: rice, grain shape, chromosome segment substitution lines, quantitative trait locus

摘要： 以一套用籼稻品种9311为受体、粳稻品种日本晴为供体构建的128个染色体片段代换系为材料，按随机区组实验设计种植，于成熟后考查代换系粒长、粒宽性状，利用多元回归分析方法，结合Bin图谱，鉴定出6个与粒长相关的QTL、2个与粒宽相关的QTL。其中，qGL3.1被定位在水稻第3染色体的5 792 954 bp区间内；qGL3.2被定位在第3染色体的917 878 bp区间内；qGL8.1被定位在第8染色体的889 543 bp区间内；qGL8.2被定位在第8染色体的208 614 bp区间内；qGL9.1被定位在第9染色体的1 149 685 bp区间内；qGL11.1被定位在第11染色体的3 184 760 bp区间内；qGW1.1被定位在第1染色体的200 070 bp区间内；qGW5.1被定位在第5染色体的704 905 bp区间内。上述QTL的准确定位，为进一步精细定位及克隆相应QTL和开展水稻粒型分子育种奠定了基础。

关键词: 水稻, 粒型, 染色体片段代换系, 数量性状基因座

CLC Number:

XU Jian-Jun1, 2 , ZHAO Qiang3, TANG Zai-Xiang1, ZHAO Yuan-Feng1, ZHU Lei1, XU Chen-Wu1, GU Ming-Hong1, HAN Bin3 . Mapping of QTLs for Grain Shape Using WholeGenome Resequenced Chromosome Segment Substitution Lines in Rice[J]. Chinese Journal of Rice Science, 2011, 25(4): 365-369.

徐建军1, 2, 赵强3, 汤在祥1, 赵元凤1, 朱磊1, 徐辰武1, 顾铭洪1, 韩斌3 . 利用重测序的染色体片段代换系群体定位水稻粒型QTL[J]. 中国水稻科学, 2011, 25(4): 365-369.

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Mapping of QTLs for Grain Shape Using WholeGenome Resequenced Chromosome Segment Substitution Lines in Rice

利用重测序的染色体片段代换系群体定位水稻粒型QTL

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