中国水稻科学
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  2008, Vol. 22 Issue (6): 597-597~602     DOI:
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Reducing Amylose Content of Hybrid Rice Xieyou 57 by Molecular MarkerAssisted Selection
CHEN Sheng 1,2, NI Dahu2, LU Xuzhong2, SONG Fengshun2, ZHANG Shilu3, WANG Zongyang4, CAI Xiuling4, LI Zefu2, WANG Xiufeng2, LI Li2, YANG Jianbo 2,*
1 School of Life Science, Anhui University, Hefei 230039, China; 2 Rice Research Institute, Anhui Academy of Agricultural Sciences /Key Laboratory of Rice Genetics and Breeding of Anhui Province, Hefei 230031, China; 3 Anqing Institute of Agricultural Sciences, Anqing 246007, China; 4 Institute of Plant Physiology & Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China; *Corresponding author, E-mail: yjianbo@263.net
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Abstract To lower the high amylose content of hybrid rice Xieyou 57 (Xieqingzao A/057), Xieqingzao B (the maintainer line of Xieqingzao A) was used as recurrent parent and crossed with a donor parent with low amylose content. PCRAcc Ⅰ molecular marker was applied during backcross to identify three Wx genotypes, i.e. GG, GT and TT. Then the genotypes, the amylose contents and agronomic traits of the four combinations, Xieqingzao B(GG)/057(GG), Xieqingzao B(GG)/057(TT), Xieqingzao B(TT)/057(GG), Xieqingzao B(TT)/057(TT) were detected, respectively. The amylose contents of the hybrids with heterozygous GTWx, i.e. Xieqingzao B(GG)/057(TT) and Xieqingzao B(TT)/057(GG) is 19.3% and 19.2% respectively, lower than that of Xieqingzao B(GG)/057(GG)(26.1%). The amylose contents of different seeds in the two hybrids with heterozygous GTWx differed greatly.The combination of Xieqingzao B(TT)/057(TT) with homozygous TTWx not only showed a decreased amylose content(12.5%) accompanied by increased gel consistency, but also had an improved uniformity in amylose contents among different seeds.
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CHEN Sheng
NI Dahu
LU Xuzhong
SONG Fengshun
ZHANG Shilu
WANG Zongyang
CAI Xiuling
LI Zefu
WANG Xiufeng
Key wordsbreeding   molecular markerassisted selection   hybrid rice   rice grain quality   amylose content     
Received: 1900-01-01;
Cite this article:   
CHEN Sheng ,NI Dahu,LU Xuzhong et al. Reducing Amylose Content of Hybrid Rice Xieyou 57 by Molecular MarkerAssisted Selection
[J]. , 2008, 22(6): 597-597~602 .
 
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