中国水稻科学
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  2010, Vol. 24 Issue (5): 493-496     DOI: 10.3969/j.issn.1001-7216.2010.05.008
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Interaction Between Rice Stripe Virus NS3 Protein and Glyceraldehyde-3-Phosphate Dehydrogenase(GAPDH) of Rice
XIAO Dong-lai;JIA Dong-sheng;WU Jian-guo; DU Zhen-guo;XIE Li-yan; WU Zu-jian*; XIE Lian-hui*
Key Laboratory of Plant Virology of Fujian Province/Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou 350002, China; *Corresponding author, E-mail: wuzujian@126.com; fjxlh@126.com
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Abstract NS3 protein of rice stripe virus (RSV) is an RNA silencing suppressor. Yeast twohybrid assay was used to screen the rice cDNA library with the NS3 protein as a bait. A novel rice protein interacting with RSV NS3 was obtained. The putative function of the interacting protein was glyceraldehyde3phosphate dehydrogenase (GAPDH). Bimolecular fluorescence complementation (BiFC) also showed that RSV NS3 could interact with the GAPDH. Cellular localization studies showed that GAPDHGFP fusion protein accumulated predominantly in cytoplasm of tobacco (Nicotiana benthamiana) through transient expression assay. Possible functions of GAPDH during the infection of rice by RSV were discussed.
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Articles by authors
XIAO Dong-lai
JIA Dong-sheng
WU Jian-guo
DU Zhen-guo
XIE Li-yan
WU Zu-jian*
XIE Lian-hui*
Key wordsrice stripe virus   protein function   interaction   yeast two hybrid   bimolecular fluorescence complementation     
Received: 1900-01-01;
Cite this article:   
XIAO Dong-lai,JIA Dong-sheng,WU Jian-guo et al. Interaction Between Rice Stripe Virus NS3 Protein and Glyceraldehyde-3-Phosphate Dehydrogenase(GAPDH) of Rice [J]. , 2010, 24(5): 493-496 .
 
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