Construction of Genetic Map andMapping andVerification of Grain TraitsQTLs Using Recombinant Inbred LinesDerived from a Cross BetweenindicaC84 andjaponicaCJ16B

doi:10.16819/j.1001-7216.2018.7120

Abstract

Abstract:

【Objective】The objective of thestudy was to layafoundation for further excavating new genes related to grain traits,analyzing the molecular mechanism and exploring the genetic regulation network of rice grain development,andprovidingtheoretical support for the molecular breeding of super rice.【Method】With the mapping population of 188-line recombinant inbred lines(RILs)derived from the cross between latejaponicarice Chunjiang16B(CJ16B) and wide-compatiblemid-indica restorationlines C84 which were relatively different in plant and grain morphology,a rice linkage genetic map was constructed on the basis of 158 polymorphic molecular markers,covering a total length of 1428.40cM and an average interval of 9.04cM.Five traits such as grain length(GL),grain width(GW),grain thickness(GT), length-to-width ratio(LWR) and thousand grain weight(TGW)were investigated in Lingshui, Hainan Province and Hangzhou, Zhejiang Province, China, and quantitative trait loci(QTLs) were analyzed.【Result】A total of 30 QTLs including 18 new QTLs were detectedand their contribution rates ranged from 3.51% to 17.25%. The number of QTLs related to GL, GW,GT, LWR and TGW were 9,5,5, 6and 5, respectively; Gene locus alignment showed that five cloned genesofgrainshape were found in the QTL marker interval, which were identified with sequencing of parental target genes and dCAPs molecular marker. 【Conclusion】The results showed that the RIL population and its genetic map could be used for the localization and cloning of the main QTLs/genes of important agronomic traits in rice.The new 18 grainQTLs are supplementto the regulation network of rice grain development.

Key words: Oryza sativa L, recombinant inbred lines, genetic map, grain traits, QTL

摘要：

【目的】本研究旨在挖掘水稻粒型新基因、探索其分子机理,解析籽粒发育调控遗传网络奠定基础,并为通过分子标记聚合有利基因开展超级稻分子设计育种提供理论依据。【方法】以植株和籽粒形态差异较大的晚粳稻品种春江16B(CJ16B）和广亲和中籼稻背景恢复系C84为亲本构建含有188个家系的重组自交系为作图群体,利用158对在双亲中存在多态性差异的分子标记,构建了遗传连锁图谱,总遗传距离为1428.40cM,平均标记间距为9.04cM。在构建遗传图谱的基础上,完成RIL188个株系籽粒的粒长、粒宽、粒厚、长宽比和千粒重等5个性状考查并进行QTL定位。【结果】在海南陵水和浙江杭州两地共检测到籽粒相关主效QTL30个,包括籽粒QTL新座位18个,解释遗传变异3.51%~17.25%。其中粒长、粒宽、粒厚和长宽比QTL位点分别为9个、5个、5个和6个,千粒重QTL位点5个。经基因座位比对,发现有5个QTL区间与已克隆的调控籽粒形态相关基因座位相近,我们通过对双亲目标基因的测序并根据差异位点设计dCAPs分子标记进行验证。【结论】该RIL群体及其遗传图谱可用于水稻重要农艺性状主效QTL基因的定位和克隆,新定位的18个粒型QTL可以为水稻籽粒发育调控网络提供补充和资料积累。

关键词: 水稻, 重组自交系, 遗传图谱, 籽粒性状, QTL

CLC Number:

Mengyu ZHOU, Xinwei SONG, Jing XU, Xue FU, Ting LI, Yuchen ZHU, Xingyun XIAO, Yijian MAO, Dali ZENG, Jiang HU, Li ZHU, Deyong REN, Zhenyu GAO, Longbiao GUO, Qian QIAN, Mingguo WU, Jianrong LIN, Guangheng ZHANG. Construction of Genetic Map andMapping andVerification of Grain TraitsQTLs Using Recombinant Inbred LinesDerived from a Cross BetweenindicaC84 andjaponicaCJ16B[J]. Chinese Journal OF Rice Science, 2018, 32(3): 207-218.

周梦玉, 宋昕蔚, 徐静, 付雪, 李婷, 朱雨晨, 肖幸运, 毛一剑, 曾大力, 胡江, 朱丽, 任德勇, 高振宇, 郭龙彪, 钱前, 吴明国, 林建荣, 张光恒. 籼稻C84和粳稻春江16B重组自交系遗传图谱构建及籽粒性状QTL定位与验证[J]. 中国水稻科学, 2018, 32(3): 207-218.

Figures/Tables 9

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性状 Trait	地点 Site	亲本Parent
性状 Trait	地点 Site	C84	春江16BCJ16B	平均Mean	范围Range
粒长 Grain length / mm	杭州 Hangzhou	8.2±0.2	7.4±0.2^**	7.74	6.4~9.0
	陵水Lingshui	7.8±0.1	7.0±0.1^**	7.56	6.5~8.7
粒宽Grain width / mm	杭州 Hangzhou	2.9±0.1	3.2±0.0^**	3.11	2.6~3.8
	陵水Lingshui	3.1±0.1	3.2±0.1^*	3.22	2.6~3.7
粒厚Grain thickness / mm	杭州 Hangzhou	1.98±0.01	2.26±0.04^**	2.06	1.79~2.38
	陵水Lingshui	2.03±0.04	2.28±0.04^**	2.08	1.75~2.34
长宽比Lengthtowidth ratio	杭州 Hangzhou	2.8±0.1	2.3±0.1^*	2.52	2.0~3.4
	陵水Lingshui	2.5±0.3	2.2±0.1^**	2.37	1.9~3.0
千粒重1000-grain weight / g	杭州 Hangzhou	23.7±0.4	25.1±0.3^*	22.17	13.8~33.2
	陵水Lingshui	24.1±0.1	25.2±0.1^*	22.98	13.3~30.8

性状 Trait	地点 Site	亲本Parent
性状 Trait	地点 Site	C84	春江16BCJ16B	平均Mean	范围Range
粒长 Grain length / mm	杭州 Hangzhou	8.2±0.2	7.4±0.2^**	7.74	6.4~9.0
	陵水Lingshui	7.8±0.1	7.0±0.1^**	7.56	6.5~8.7
粒宽Grain width / mm	杭州 Hangzhou	2.9±0.1	3.2±0.0^**	3.11	2.6~3.8
	陵水Lingshui	3.1±0.1	3.2±0.1^*	3.22	2.6~3.7
粒厚Grain thickness / mm	杭州 Hangzhou	1.98±0.01	2.26±0.04^**	2.06	1.79~2.38
	陵水Lingshui	2.03±0.04	2.28±0.04^**	2.08	1.75~2.34
长宽比Lengthtowidth ratio	杭州 Hangzhou	2.8±0.1	2.3±0.1^*	2.52	2.0~3.4
	陵水Lingshui	2.5±0.3	2.2±0.1^**	2.37	1.9~3.0
千粒重1000-grain weight / g	杭州 Hangzhou	23.7±0.4	25.1±0.3^*	22.17	13.8~33.2
	陵水Lingshui	24.1±0.1	25.2±0.1^*	22.98	13.3~30.8

地点Site 性状Trait		HZ-GL	HZ-GW	HZ-GT	HZ-LWR	HZ-TGW	LS-GL	LS-GW	LS-GT	LS-LWR
杭州	粒宽 GW	–0.104^**
Hangzhou	粒厚 GT	0.073^**	0.396^**
(HZ)	长宽比LWR	0.672^**	–0.801^**	–0.248^**
	千粒重TGW	0.441^**	0.443^**	0.404^**	–0.066^**
陵水	粒长 GL	0.809^**
Lingshui	粒宽 GW		0.817^**				–0.016^**
(LS)	粒厚 GT			0.270^**			0.117^**	0.164^**
	长宽比LWR				0.854^**		0.660^**	–0.758^**	–0.030^**
	千粒重 TGW					0.529^**	0.438^**	0.508^**	0.287^**	–0.094^**

地点Site 性状Trait		HZ-GL	HZ-GW	HZ-GT	HZ-LWR	HZ-TGW	LS-GL	LS-GW	LS-GT	LS-LWR
杭州	粒宽 GW	–0.104^**
Hangzhou	粒厚 GT	0.073^**	0.396^**
(HZ)	长宽比LWR	0.672^**	–0.801^**	–0.248^**
	千粒重TGW	0.441^**	0.443^**	0.404^**	–0.066^**
陵水	粒长 GL	0.809^**
Lingshui	粒宽 GW		0.817^**				–0.016^**
(LS)	粒厚 GT			0.270^**			0.117^**	0.164^**
	长宽比LWR				0.854^**		0.660^**	–0.758^**	–0.030^**
	千粒重 TGW					0.529^**	0.438^**	0.508^**	0.287^**	–0.094^**

染色体 Chromosome	物理距离 Physical distance/Mb	遗传距离 Genetic distance /cM	平均物理距离 Mean physical distance /Mb	平均遗传距离 Mean genetic distance /cM	标记数 Number of markers
1	46.15	192.8	2.71	11.34	17
2	35.93	128.0	2.57	9.14	14
3	36.41	145.9	2.28	9.12	16
4	35.28	148.5	1.96	8.25	18
5	29.89	106.9	1.99	7.13	15
6	31.25	104.8	2.84	9.53	11
7	29.69	128.5	2.12	9.18	14
8	28.44	107.2	2.59	9.75	11
9	23.01	77.5	2.30	7.75	10
10	23.13	72.5	2.57	8.06	9
11	28.51	115.5	2.38	9.63	12
12	27.49	100.3	2.50	9.12	11
合计 Total	375.18	1428.4	2.40	9.04	158