Chinese Journal OF Rice Science ›› 2019, Vol. 33 ›› Issue (1): 28-34.DOI: 10.16819/j.1001-7216.2018.8036
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Yan ZHAO*(), Yongzhou TANG, Yuqian SHI
Received:
2018-03-28
Revised:
2018-07-04
Online:
2019-01-10
Published:
2019-01-10
Contact:
Yan ZHAO
通讯作者:
赵艳
基金资助:
CLC Number:
Yan ZHAO, Yongzhou TANG, Yuqian SHI. Rice Seed-specific Glutenin GluB1 Promoter Drives Exogenous Gene Expression in Rice Callus[J]. Chinese Journal OF Rice Science, 2019, 33(1): 28-34.
赵艳, 唐湧洲, 史玉倩. 水稻种子特异性谷蛋白GluB1启动子在水稻愈伤组织中驱动外源基因表达[J]. 中国水稻科学, 2019, 33(1): 28-34.
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URL: http://www.ricesci.cn/EN/10.16819/j.1001-7216.2018.8036
Fig. 1. Sketch map of T-DNA of pCAMBIA1302-pGluB1sig-CTBIN-NOS vector. T BORDER(L), The left boundary; T BORDER(R), The right boundary; 35S, Cauliflower mosaic virus 35S promoter; hpt, The hygromycin B phosphotransferase gene; NOS, Nopaline synthase terminator; pGluB1, Rice glutelin GluB1 promoter of 2.3 kb; signal, Rice glutelin GluB1 signal peptide gene sequence; CTBIN, Artificially designed and synthesized fusion gene sequence of cholera toxin B subunit and human proinsulin, with the KDEL ER retention signal gene sequence at the C terminus; mGFP, Modified green fluorescent protein gene.
Fig. 3. PCR analysis of CTBIN gene in rice transgenic callus clones. M, DL2000 DNA marker; P, Plasmid(positive control); CK, Nontransgenic callus clone(negative control); 1-7, Number of different transgenic rice calli clones.
Fig. 5. Detection of transcriptional expression of CTBIN gene in transgenic rice calli by RT-PCR. The upper part shows the amplified bands of purpose gene CTBIN, the lower part the amplified bands of rice inner control gene β-actin. M, DL2000 DNA marker; CK, Nontransgenic rice calli clone(negative control); 1-7, Number of different transgenic rice calli clones.
Fig. 6. Detection of translational expression of CTBIN gene in transgenic rice calli by Western blot. CK, Nontransgenic rice calli clone (negative control); 1, 2, 4 and 5, Number of different transgenic rice calli clones.
[1] | de Los Reyes B G, Mohanty B, Yun S J, Park M R, Lee D Y. Upstream regulatory architecture of rice genes: Summarizing the baseline towards genus-wide comparative analysis of regulatory networks and allele mining.Rice, 2015, 8(1): 14. |
[2] | Ye R, Zhou F, Lin Y.Two novel positive cis-regulatory elements involved in green tissue-specific promoter activity in rice (Oryza sativa L. ssp.). Plant Cell Rep, 2012, 31(7): 1159-1172. |
[3] | Fumio T, Yuhya W, Shimpei H.An overview on the strategies to exploit rice endosperm as production platform for biopharmaceuticals.Plant Sci, 2017, 263: 201-209. |
[4] | Chen Y, Sun A,Wang M, Zhu Z, Ouwerkerk P B F. Functions of the CCCH type zinc finger protein OsGZF1 in regulation of the seed storage protein GluB-1 from rice.Plant Mol Biol, 2014, 84: 621-634. |
[5] | Qu L Q, Takaiwa F.Evaluation of tissue specificity and expression strength of rice seed component gene promoters in transgenic rice.Plant Biotech J, 2004, 2(2): 113-125. |
[6] | 张宪银, 薛庆中. 水稻胚乳特异性启动子Gt1的克隆及其功能验证. 作物学报, 2002, 28(1): 110-114. |
Zhang X Y, Xue Q Z.Cloning of a rice endosperm specific promoter Gt1 and its functional verification.Acta Agron Sin, 2002, 28(1):110-114. (in Chinese with English abstract). | |
[7] | 刘峰, 赵伊英, 郑金贵. 籼稻胚乳特异性启动子Gt1的克隆及其功能验证. 长江大学学报:自然科学版, 2010, 7(2): 44-48. |
Liu F, Zhao Y Y, Zheng J G.Isolation of endosperm-specific promoter Gt1 from indica rice and verification of its function in transgenic rice.J Yangtze Univ: Nat Sci Ed, 2010, 7(2): 44-48. (in Chinese with English abstract). | |
[8] | Takaiwa F, Yang L, Wakasa Y, Ozawa K.Compensatory rebalancing of rice prolamins by production of recombinant prolamin/bioactive peptide fusion proteins within ER-derived protein bodies.Plant Cell Rep, 2018, 37: 209-223. |
[9] | Takaiwa F, Takagi H, Hirose S, Wakasa Y.Endosperm tissue is good production platform for artificial recombinant proteins in transgenic rice.Plant Biotechnol J, 2007, 5: 84-92. |
[10] | Katsube T, Kurisaka N, Ogawa M, Maruyama N, Ohtsuka R, Utsumi S, Takaiwa F.Accumulation of soybean glycinin and its assembly with the glutelins in rice.Plant Physiol, 1999, 120(4): 1063-1074. |
[11] | Momma K, Hashimoto W, Ozawa S, Kawai S, Katsube T, Takaiwa F, Kito M, Utsumi S, Murata K.Quality and safety evaluation of genetically engineered rice with soybean glycinin: analyses of the grain composition and digestibility of glycinin in transgenic rice.Biosci, Biotechnol& Biochem, 1999, 63(2): 314-318. |
[12] | Takagi H, Saito S, Yang L, Nagasaka S, Nishizawa N, Takaiwa F.Oral immunotherapy against a pollen allergy using a seed-based peptide vaccine.Plant Biotechnol J, 2005, 3(5): 521-533. |
[13] | Chesnokov Y V, Manteuffel R.Dose effect of UV-B irradiation on pollen tube growth and seed-specific promoter activities in irradiated pollen grains of Nicotiana plumbaginifolia. Sex Plant Reprod, 2000, 12(6): 361-364. |
[14] | Russell D A, Fromm M E.Tissue-specific expression in transgenic maize of four endosperm promoters from maize and rice.Transgenic Res, 1997, 6(2): 157-168. |
[15] | 唐湧洲, 俞越, 赵艳. 种子蛋白体靶向表达口服重组胰岛素原载体构建及转化水稻. 中国水稻科学, 2015, 29(5): 481-489. |
Tang Y Z, Yu Y, Zhao Y.Construction of seed protein body targeted expression vector and transformation rice for oral recombinant proinsulin.Chin J Rice Sci, 2015, 29(5): 481-489. (in Chinese with English abstract) | |
[16] | 陈惠, 赵原, 种康. 一种改进的水稻成熟胚愈伤组织高效基因转化系统. 植物学通报, 2008, 25(3): 322-331. |
Chen H, Zhao Y, Zhong K.Improved high efficient system for rice transformation using mature embryo-derived calli.Chin Bull Bot, 2008, 25(3): 322-331. (in Chinese with English abstract) | |
[17] | 卢江扬, 郑康乐. 提取水稻DNA的一种简易方法. 中国水稻科学,1992, 6(1): 47-48. |
Lu J Y, Zheng K L.A simple method for isolation of rice DNA.Chin J Rice Sci, 1992, 6(1): 47-48. (in Chinese with English abstract) | |
[18] | Jang I C, Nahm B H, Kim J K.Subcellular targeting of green fluorescent protein to plastids in transgenic rice plants provides a high-level expression system.Mol Breeding, 1999, 5(5): 453-461. |
[19] | Bradford M M.A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.Anal Biochem, 1976, 72(1): 248-254. |
[20] | Takaiwa F, Hirose S, Takagi H, Yang L, Wakasa Y.Deposition of a recombinant peptide in ER-derived protein bodies by retention with cysteine-rich prolamins in transgenic rice seed.Planta, 2009, 229(5): 1147-1158. |
[21] | Jiang S Y, Vanitha J, Bai Y, Ramachandran S.Identification and molecular characterization of tissue- preferred rice genes and their upstream regularly sequences on a genome-wide level.BMC Plant Biol, 2014, 14: 331-345. |
[22] | Wu C, Washida H, Onodera Y, Harada K, Takaiwa F.Quantitative nature of the prolamin-box, ACGT and AACA motifs in a rice glutelin gene promoter: Minimal cis-element requirements for endosperm-specific gene expression. Plant J, 2000, 23(3): 415-421. |
[23] | Onodera Y, Suzuki A, Wu C Y, Washida H, Takaiwa F.A rice functional transcriptional activator, RISBZ1, responsible for endosperm-specific expression of storage protein genes through GCN4 motif.J Biol Chem, 2001, 276(17): 14139-14152. |
[24] | Suzuki A, Wu C Y, Washida H, Takaiwa H.Rice MYB protein OSMYB5 specifically binds to the AACA motif conserved among promoters of genes for storage protein glutelin.Plant Cell Physiol, 1998, 39(5): 555-559. |
[25] | 张东向. 禾本科植物愈伤组织的形成分化和体细胞胚胎发生. 齐齐哈尔大学学报: 自然科学版, 2002, 18(4): 112-115. |
Zhang D X.Differentiation and somatic embryo genesis of forming gallus of Gramineae.J Qiqihar Univ: Nat Sci Ed, 2002, 18(4): 112-115. (in Chinese with English abstract) | |
[26] | Duan M, Sun S S.Profiling the expression of genes controlling rice grain quality.Plant Mol Biol, 2005, 59(1): 165-178. |
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