中国水稻科学

中国水稻科学

• 研究报告 • 上一篇    下一篇

稻瘟病菌温度敏感型突变体的筛选

王洪凯1; 林福呈2; 柴荣耀3; 李德葆1   

  1. 1浙江大学 生物技术研究所, 浙江 杭州 310029； 2浙江大学 生命科学学院, 浙江 杭州 310029； 3浙江省农业科学院 植物保护与微生物研究所, 浙江 杭州 310021
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2004-07-10 发布日期:2004-07-10

Screening of Temperature Sensitive Mutants in Magnaporthe grisea

WANG Hong-kai 1; LIN Fu-cheng 2; CHAI Rong-yao 3; LI De-bao 1   

  1. （ 1 Biotechnology Institute; Zhejiang University; Hangzhou 310029; China; 2 College of Life Sciences; 3 Institute of Plant Protection and Microbiology; Zhejiang Academy of Agricultural Sciences; Hangzhou 310021; China）
  • Received:1900-01-01 Revised:1900-01-01 Online:2004-07-10 Published:2004-07-10

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摘要： 通过紫外诱变，筛选获得了274个稻瘟病菌的温度敏感型突变体，这些突变体在25℃下可以生长，而在33℃下不能生长。致病性测定结果表明，有32个突变体的致病性显著降低; 在33℃条件下，不萌发的有7个；有12个萌发后停止生长，不能形成附着胞；有9个可以形成附着胞，以后停止生长；有4个形成膨大透明且成串生长的附着胞，随后停止生长。有些温度敏感型突变体形成子囊壳的能力也发生了改变。

关键词: 稻瘟病菌, 温度敏感型突变体, 萌发, 附着胞, 子囊壳

Abstract: Temperature sensitive mutants(ts mutants) are good material for studying cell division and differentiation. Two hundred and seventy four ts mutants of Magnaporthe grisea were induced by UV radiation. These mutants could grow at 25℃, but not at 33℃. Pathogenicity test indicated that the pathogenicity of 32 ts mutants reduced distinctly. Under 33℃ conditions, seven of them did not germinate, 12 stopped growing after germination and no appressorium formed, nine stopped growing after appressorium formed, four mutants formed swell and bunch appressorium, then stopped growing. The ability of perithecia formation of some mutants was changed.

Key words: Magnaporthe grisea, temperature sensitive mutant, germination, appressorium, perithecia