中国水稻科学

中国水稻科学

• 研究简报 • 上一篇    

二化螟对氟虫腈抗性初探

姜卫华;韩召军;郝鸣丽   

  1. 南京农业大学 农业部病虫监测与治理重点开放实验室， 江苏 南京 210095
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2005-11-10 发布日期:2005-11-10

Primary Study on Resistance of Rice Stem Borer (Chilo suppressalis) to Fipronil

JIANG Wei-hua , HAN Zhao-jun , HAO Ming-li   

  • Received:1900-01-01 Revised:1900-01-01 Online:2005-11-10 Published:2005-11-10

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被引次数

120

摘要： 采用点滴测定法，于2001~2004年对江、浙、皖3省12个县市的二化螟进行了氟虫腈的抗性调查。江苏及安徽等地二化螟对氟虫腈仍处于敏感状态，但江苏无锡和浙江慈溪二化螟的敏感性明显下降（抗性倍数RR分别为3.1和3.6倍），浙江苍南二化螟已出现中等水平的抗性（RR为21.2倍）。由此认为，二化螟目前仍处于对氟虫腈抗性发展的早期阶段。解毒酶抑制剂的活体增效实验发现，TPP、DEM能明显提高氟虫腈对抗性二化螟的毒力（增效比SR分别为1.8和1.6），但PBO在敏感性和抗性二化螟中的增效作用均不显著（SR为1.1~1.2）。表明酯酶和谷胱甘肽转移酶与二化螟对氟虫腈抗性的形成有一定关系。不同杀虫剂的比较毒力测定表明，三唑磷和敌百虫由于产生了抗性，对二化螟的毒力很低，乙酰甲胺磷本身对二化螟的毒力也不高，已很难再用来防治抗性二化螟。但哒嗪硫磷、二嗪磷、溴氰菊酯和阿维菌素对二化螟的毒力较高，而且尚没有明显的抗性产生，在二化螟抗性治理中，可以用作氟虫腈的轮换防治药剂。

关键词: 二化螟, 氟虫腈, 抗药性, 相对毒力

Abstract: By means of topical application, fipronil resistance was surveyed in the rice stem borer, Chilo suppressalis,from 12 diferent areas in East China from 2001 to 2004. The rice stem borers in most regions of Jiangsu and Anhui were still susceptible to fipronil. But in Wuxi of Jiangsu and Cixi of Zhejiang, their sensitivity decreased (resistance ratios were 3.1 and 3.6), and the moderate level of resistance (resistance ratio was 21.2) was found in the population of Cangnan, Zhejiang. Therefore it was concluded that the pest resistant to fipronil was still at the early developmental stage. Synergism experiments showed that PBO had little effect on both susceptible and resistant borers (synergism ratios were 1.1 to 1.2). Though TPP and DEM had no effect on susceptible ones, they had significant synergism to fipronil in resistant borers (synergism ratios were 1.8 and 16, respectively), indicating esterase and GSTs might be involved in the resistance mechanism. Bioassay with currently used insecticides revealed that triazophos (because of high resistance), trichlorphon and acephate had very low toxicity to resistant borers, while diazinon, pyridaphenthion, decamethrin and avermectin showed high toxicity and had no cross resistance to fipronil, which could be considered as substitute insecticides in the resistance managment.

Key words: rice stem borer, fipronil, resistance, relative toxicity