中国水稻科学

中国水稻科学

• 研究简报 • 上一篇    

几种野生稻甲醇提取物对害虫的行为干扰及对酯酶同工酶的影响

万树青1;刘祥发1; 冯国忠1;潘大建2   

  1. 1华南农业大学 农药与化学生物学教育部重点实验室， 广东 广州 510642； E-mail:wanshuqing1953@yahoo.com.cn； 2广东省农业科学院 水稻研究所， 广东 广州 510640
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2006-05-10 发布日期:2006-05-10

Deterrent Activity of Extracts from Several Species of Wild Rice to Panonychus citri and Aphis citricola in Associated with Esterase Isoenzyme in Pests

WAN Shu-qing , LIU Xiang-fa , FENG Guo-zhong , PAN Da-jian
  

  • Received:1900-01-01 Revised:1900-01-01 Online:2006-05-10 Published:2006-05-10

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被引次数

4

摘要： 采用索氏回流法，经甲醇提取，获得大颖野生稻（E63/64、E61）、小粒野生稻（E139、E1313）、药用野生稻（E158、E1513）、斑点野生稻（E161、E163、E1613）、阔叶野生稻（101392、E91、E910）和颗粒野生稻（E74）甲醇提取物。室内生物活性测定表明这些野生稻甲醇提取物对柑橘全爪螨和绣线菊蚜具有一定的忌避活性，其中药用野生稻E158的甲醇抽提物效果更为显著，5×104 μg/mL的浓度处理，测得24 h和48 h对柑橘全爪螨的忌避率分别为83.26%和87.95%；用同样的浓度处理，对绣线菊蚜的忌避率分别为87.86%和82.43%。经聚丙烯酰胺凝胶电泳检测，药用野生稻E158的甲醇抽提物对两种害虫的酯酶同工酶具有明显的抑制效果。

关键词: 野生稻, 柑橘全爪螨, 绣线菊蚜, 忌避作用, 酯酶同工酶, 提取物, 生物防治

Abstract: Six species of wild rice and their different ecotypes including Oryza grandiglumis (E6-1, E6-3/6-4), O.minuta (E13-9, E13-13), O. officinalis (E15-8, E15-13), O.punctata(E16-1, E16-3, E16-13), O.granulata(E7-4), O.latifolia(101392, E9-1, E9-10) were extracted with methanol. Deterrent activity of the extracts to Aphis citricola and Panonychus citri was tested. The extract from the wild rice O.officinalis E15-8 was better than the others in the deterrent activity to the two pests. The deterrent rates of E15-8 to Panonychus citri and Aphis citricola were 83.26% and 87.86% in 24 h and 87.95% and 82.43% in 48 h at 5×10 4 μg/mL concentration, respectively. The methanol extracts from O. officinalis E15-8 had the effect of inhibition to the esterase of the two pests.

Key words: wild rice, Panonychus citri, Aphis citricola, deterrent activity, esterase isoenzyme, extracts, biological control