江淮流域杂草稻叶绿体DNA的籼粳分化

doi:10.3969/j.issn.1001-7216.2009.04.09

摘要/Abstract

摘要： 为了探明江淮流域杂草稻的细胞质来源，根据水稻叶绿体DNA ORF100（Open Reading Frame 100）序列在籼粳间存在69 bp差异的特征，设计了InDel标记cpDNA69。利用该标记对2个分别以籼稻和粳稻为母本的F2群体、22份栽培稻（Oryza sativa L.）、3份普通野生稻（O. rufipogon Griff.）进行了分析验证。PCR结果可以获得缺失和非缺失两种带型，缺失带型与以籼稻为母本的F2群体、10份籼稻材料完全对应；非缺失带型与以粳稻为母本的F2群体、11份粳稻材料完全对应，3份广西普通野生稻为非缺失带型，属粳型，1份以粳稻为母本的籼粳杂交材料为粳型。因此，cpDNA69可以用作叶绿体籼粳鉴定标记。利用该标记对22份杂草稻的叶绿体DNA鉴定的结果表明，7份早年发现的杂草稻，即江苏省连云港穭稻和安徽省怀远、来安、全椒、肥东的塘稻的叶绿体DNA为非缺失带型，属粳型，而近年来在江苏省扬中、高邮、灌云、洪泽、盐都、兴化、如皋等地直播稻田发现的15份红米杂草稻的叶绿体DNA为缺失带型，属籼型。这为进一步研究江淮流域杂草稻的来源提供了依据。

关键词: 杂草稻, 栽培稻, 叶绿体DNA, 籼粳分化, 细胞质来源

Abstract: To detect the cytoplasmic origin of weedy rice in the ChangjiangHuaihe River Valley of China, an InDel marker cpDNA69 was developed based on the 69 bp deletion in indica chloroplast DNA ORF100(cpDNA Open Reading Frame 100). Three accessions of common wild rice (Oryza rufipogon Griff.) from Guangxi, China and 22 cultivars (O. sativa L.) and two F2 populations with indica and japonica rice as maternal parent respectively were investigated by the length polymorphism for ORF100 region. The nondeletion cpDNA ORF100 was found in japonica cultivars and the F2 plants with japonica rice as maternal parent, and the deletion cpDNA ORF100 was found in the indica cultivars and the F2 plants with indica rice as maternal parent. The three accessions of common wild rice carried the nondeletion cpDNA type and one indicalike cultivar developed from the hybrid of japonica and indica carried the same nondeletion cpDNA type. These results confirm that cpDNA69 was a perfect marker to distinguish the indica/japonica cpDNA ORF100. Then the indicajaponica differentiation of chloroplast DNA in 22 accessions of weedy rice from the Changjiang and Huaihe River Valley were investigated with cpDNA69. The cpDNA of Ludao from Lianyungang, Jiangsu Province, and Tangdao from Huaiyuan, Laian, Quanjiao, Feidong, Anhui Province carried nondeletion type ORF100. However the cpDNA of red weedy rice discovered in recent years in Jiangsu Province carried the 69 bp deletion type ORF100. It is suggested that the indicajaponica differentiation of cpDNA has occured in weedy rice in the Changjiang and Huaihe River Valley.

Key words: weedy rice, cultivated rice, chloroplast DNA, indicajaponica differentiation, cytoplasmic origin, molecular marker

杨杰,王军,曹卿,陈志德,汤陵华,王艳萍,方先文,王才林,仲维功. 江淮流域杂草稻叶绿体DNA的籼粳分化[J]. 中国水稻科学 2009,23(4): 391-397 . , DOI: 10.3969/j.issn.1001-7216.2009.04.09 .

YANG Jie,WANG Jun,CAO Qing,CHEN Zhide,TANG Linghua,WANG Yan ping,FANG Xianwen,WANG Cailin,ZHONG Weigong*. Indicajaponica Differentiation of Chloroplast DNA of Weedy Rice in the Changjiang and
Huaihe River Valley of China[J]. Chinese Journal of Rice Science 2009,23(4): 391-397 ., DOI: 10.3969/j.issn.1001-7216.2009.04.09 .

参考文献

[1]Mortimer M, Pandey S, Piggin C. Weedy rice: Approaches to ecological appraisal and implications for research priorities//Baki B B, Chin D V, Mortimer M. Proceedings of Wild and Weedy Rice in Rice Ecosystems in Asia. Manila, Philippines: IRRI, 2000: 97-105.
[2]Noldin J A. Red rice status and management in the America//Baki B B, Chin D V, Mortimer M. Proceedings of Wild and Weedy Rice in Rice Ecosystems in Asia. Manila, Philippines: IRRI, 2000: 21-24.
[3]Zhang C X. Wild and weedy rice in China//Baki B B, Chin D V, Mortimer M. Proceedings of Wild and Weedy Rice in Rice Ecosystems in Asia. Manila, Philippnies: IRRI, 2000: 35.
[4]Olsen K M, Caicedo A L, Jia Y L. Evolutionary genomics of weedy rice in the USA. J Integr Plant Biol, 2007, 49(6): 811-816.
[5]Tang L H, Hiroko M. Genetics characterization of weedy rices and the inference on their origins. Breeding Sci, 1997, 47: 153-160.
[6]Vaughan L K, Ottis B V, PrazakHavey A M, et al. Is all red rice found in commercial rice really Oryza sativa? Weed Sci, 2001, 49: 468-476.
[7]许聪, 吴万春. 杂草稻的分类地位和利用途径探讨. 海南大学学报:自然科学版, 1996, 14（2）： 146-151.
[8]Ferrero A, Vidotto F, Balsari P, et al. Mechanical and chemical control of red rice (Oryza sativa L. var. sylvativa) in rice (Oryza sativa L.) preplanting. Crop Prot, 1999, 18: 245-251.
[9]马殿荣, 李茂柏, 王楠, 等．中国辽宁省杂草稻遗传多样性及群体分化研究. 作物学报, 2008, 34(3): 403-411.
[10] 杨杰, 仲维功, 陈志德, 等. 江苏省扬中杂草稻生物学特性初步研究. 杂草科学, 2007(3): 13-152.
[11] Hirai A, Ishibash T, Marlkami A, et al．Rice chloroplast DNA: A physical map and the location at the genes for the large subunit of ribulose 1,5bisphosphate carboxylase and the 32 kD photosystem Ⅱ reaction center protein. Theor Appl Genet, 1985, 70: 117-122.
[12]Hiratsuka J, Shimada H, Whittier R, et al．The complete sequence of the rice (Oryza sativa) chloroplast genome: Intermolecular recombination between distinct tRNA genes accounts for a maior plastid DNA inversion during the evolution of the cereals. Mol Gen Genet, 1989, 21(7)： 185-194.
[13]Ishii T, Terachi T, Tsunewaki K．Restriction endonuchiase analysis of chloroplast DNA from cultivated rice species, Oryza sativa and O．glaberrima. Jpn J Genet, 1986, 61: 537-541.
[14]Ishii T, Terachi T, Tsunewaki K. Restriction endonuclease analysis of chloroplast DNA from a genome diploid species of rice. Jpn J Genet, 1988, 63: 523-536.
[15]Kanno A, Watanabe N, Nakamura I, et al. Variation in chloroplast DNA from rice(Oryza sativa)： Differences between deletions mediated by short direct repeat sequences within a single species. Theor Appl Genet, 1993, 86: 579-584.
[16]Chen W B, Nakamura I, Sato Y I, et al. Distribution of deletion type in cpDNA of cultivated and wild rice. Jpn J Genet, 1993, 68: 597603.
[17]Chen W B, Nakamura I, Sato Y I, et al. Indicajaponica differentiation in Chinese rice landraces. Euphytica, 1994, 74: 195-201.
[18]Sato Y I. Variation in spikelet shape of the indica and japonica rice cultivars in Asian origin.Jpn J Breed, 1991, 41: 121-134.
[19]Sun C Q, Wang X K, Yoshimura A, et al. Genetic differentiation or nuclear, mitochondrial and chloroplast genomes in common wild rice (Oryza rufipogon Griff.) and cultivated rice (Oryza sativa L.). Theor Appl Genet, 2002, 104: 1335-1345.
[20]Tang J B, Xia H A, Cao M L, et al. A comparison of rice chloroplast genomes. Plant Physiol, 2004, 135: 412-420.
[21]江苏省农科院粮食所品种资源室.连云港地区穭稻初步考查. 江苏农业科学, 1984(3): 46.
[22]Chen J J, Ding J H, Ouyang Y D, et al. A triallelic system of S5 is a major regulator of the reproductive barrier and compatibility of indicajaponica hybrids in rice. Proc Natl Acad Sci USA, 2008, 105(32): 11436-11441.
[23]Dally A M, Second G．Chloroplast DNA diversity in wild and cultivated species of rice(Genus Oryza, Section Oryza): Cladisticmutation and geneticdistance analysis．Theor Appl Genet, 1990, 80： 209-222.
[24]Andersen J R, Lübberstedt T. Functional markers in plants. Trends Plant Sci, 2003, 8(11): 554-560.
[25] 蒋菏, 吴竞仑, 王根来. 连云港穭稻研究. 作物品种资源, 1985(2)： 4-7.
[26]魏兴华, 杨致荣, 董岚, 等. 穭稻分类地位的SSR证据. 中国农业科学, 2004, 37（7）： 937-942.
[27]Zhu S S, Jiang L, Wang C M, et al. The origin of weedy rice Ludao in China deduced by genome wide analysis of its hybrid sterility genes. Breeding Sci, 2005, 55(4): 409-414.
[28]Jing W, Zhang W, Jiang L, et al. Two novel loci for pollen sterility in hybrids between the weedy strain Ludao and the japonica variety Akihikari of rice (Oryza sativa L.). Theor Appl Genet, 2007, 114: 915-925.
[29]仲维功, 杨杰, 陈志德, 等. 江苏扬中杂草稻的籼粳分类. 江苏农业学报, 2006, 22（3）： 238-242.
[30]许聪, 吴万春. 海南岛杂草稻的生态考察和鉴定. 中国水稻科学, 1996, 10(4): 247-249.