中国水稻科学
     首页  |  期刊介绍  |  编 委 会  |  投稿指南  |  期刊订阅  |  广告服务  |  留言板  |  联系我们  |  English
中国水稻科学  2009, Vol. 23 Issue (4): 391-397     DOI: 10.3969/j.issn.1001-7216.2009.04.09
研究报告 最新目录 | 下期目录 | 过刊浏览 | 高级检索  |   
江淮流域杂草稻叶绿体DNA的籼粳分化
杨 杰,王 军,曹 卿,陈志德,汤陵华,王艳萍,方先文,王才林,仲维功*
江苏省农业科学院 粮食作物研究所, 江苏 南京 210014; *通讯联系人, E-mail: wgzhong0503@yahoo.com.cn
Indicajaponica Differentiation of Chloroplast DNA of Weedy Rice in the Changjiang and
Huaihe River Valley of China
YANG Jie, WANG Jun, CAO Qing, CHEN Zhide, TANG Linghua, WANG Yan ping, FANG Xianwen, WANG Cailin, ZHONG Weigong*
Institute of Food Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China; * Corresponding author, E-mail:wgzhong0503@yahoo.com.cn
 全文: PDF (2751 KB)   HTML (1 KB)   输出: BibTeX | EndNote (RIS)      背景资料
摘要 为了探明江淮流域杂草稻的细胞质来源,根据水稻叶绿体DNA ORF100(Open Reading Frame 100)序列在籼粳间存在69 bp差异的特征,设计了InDel标记cpDNA69。利用该标记对2个分别以籼稻和粳稻为母本的F2群体、22份栽培稻(Oryza sativa L.)、3份普通野生稻(O. rufipogon Griff.)进行了分析验证。PCR结果可以获得缺失和非缺失两种带型,缺失带型与以籼稻为母本的F2群体、10份籼稻材料完全对应;非缺失带型与以粳稻为母本的F2群体、11份粳稻材料完全对应,3份广西普通野生稻为非缺失带型,属粳型,1份以粳稻为母本的籼粳杂交材料为粳型。因此,cpDNA69可以用作叶绿体籼粳鉴定标记。利用该标记对22份杂草稻的叶绿体DNA鉴定的结果表明,7份早年发现的杂草稻,即江苏省连云港穭稻和安徽省怀远、来安、全椒、肥东的塘稻的叶绿体DNA为非缺失带型,属粳型,而近年来在江苏省扬中、高邮、灌云、洪泽、盐都、兴化、如皋等地直播稻田发现的15份红米杂草稻的叶绿体DNA为缺失带型,属籼型。这为进一步研究江淮流域杂草稻的来源提供了依据。
服务
把本文推荐给朋友
加入我的书架
加入引用管理器
E-mail Alert
RSS
作者相关文章
杨 杰
王 军
曹 卿
陈志德
汤陵华
王艳萍
方先文
王才林
仲维功
关键词杂草稻   栽培稻   叶绿体DNA   籼粳分化   细胞质来源     
AbstractTo detect the cytoplasmic origin of weedy rice in the ChangjiangHuaihe River Valley of China, an InDel marker cpDNA69 was developed based on the 69 bp deletion in indica chloroplast DNA ORF100(cpDNA Open Reading Frame 100). Three accessions of common wild rice (Oryza rufipogon Griff.) from Guangxi, China and 22 cultivars (O. sativa L.) and two F2 populations with indica and japonica rice as maternal parent respectively were investigated by the length polymorphism for ORF100 region. The nondeletion cpDNA ORF100 was found in japonica cultivars and the F2 plants with japonica rice as maternal parent, and the deletion cpDNA ORF100 was found in the indica cultivars and the F2 plants with indica rice as maternal parent. The three accessions of common wild rice carried the nondeletion cpDNA type and one indicalike cultivar developed from the hybrid of japonica and indica carried the same nondeletion cpDNA type. These results confirm that cpDNA69 was a perfect marker to distinguish the indica/japonica cpDNA ORF100. Then the indicajaponica differentiation of chloroplast DNA in 22 accessions of weedy rice from the Changjiang and Huaihe River Valley were investigated with cpDNA69. The cpDNA of Ludao from Lianyungang, Jiangsu Province, and Tangdao from Huaiyuan, Laian, Quanjiao, Feidong, Anhui Province carried nondeletion type ORF100. However the cpDNA of red weedy rice discovered in recent years in Jiangsu Province carried the 69 bp deletion type ORF100. It is suggested that the indicajaponica differentiation of cpDNA has occured in weedy rice in the Changjiang and Huaihe River Valley.
Key wordsweedy rice   cultivated rice   chloroplast DNA   indicajaponica differentiation   cytoplasmic origin   molecular marker   
收稿日期: 1900-01-01;
引用本文:   
杨 杰,王 军,曹 卿等. 江淮流域杂草稻叶绿体DNA的籼粳分化[J]. 中国水稻科学, 2009, 23(4): 391-397 .
YANG Jie,WANG Jun,CAO Qing et al. Indicajaponica Differentiation of Chloroplast DNA of Weedy Rice in the Changjiang and
Huaihe River Valley of China
[J]. , 2009, 23(4): 391-397 .
 
[1] Mortimer M, Pandey S, Piggin C. Weedy rice: Approaches to ecological appraisal and implications for research priorities//Baki B B, Chin D V, Mortimer M. Proceedings of Wild and Weedy Rice in Rice Ecosystems in Asia. Manila, Philippines: IRRI, 2000: 97-105.[2]Noldin J A. Red rice status and management in the America//Baki B B, Chin D V, Mortimer M. Proceedings of Wild and Weedy Rice in Rice Ecosystems in Asia. Manila, Philippines: IRRI, 2000: 21-24.[3]Zhang C X. Wild and weedy rice in China//Baki B B, Chin D V, Mortimer M. Proceedings of Wild and Weedy Rice in Rice Ecosystems in Asia. Manila, Philippnies: IRRI, 2000: 35.[4]Olsen K M, Caicedo A L, Jia Y L. Evolutionary genomics of weedy rice in the USA. J Integr Plant Biol, 2007, 49(6): 811-816.[5]Tang L H, Hiroko M. Genetics characterization of weedy rices and the inference on their origins. Breeding Sci, 1997, 47: 153-160.[6]Vaughan L K, Ottis B V, PrazakHavey A M, et al. Is all red rice found in commercial rice really Oryza sativa? Weed Sci, 2001, 49: 468-476.[7]许聪, 吴万春. 杂草稻的分类地位和利用途径探讨. 海南大学学报:自然科学版, 1996, 14(2): 146-151.[8]Ferrero A, Vidotto F, Balsari P, et al. Mechanical and chemical control of red rice (Oryza sativa L. var. sylvativa) in rice (Oryza sativa L.) preplanting. Crop Prot, 1999, 18: 245-251.[9]马殿荣, 李茂柏, 王楠, 等.中国辽宁省杂草稻遗传多样性及群体分化研究. 作物学报, 2008, 34(3): 403-411.[10] 杨杰, 仲维功, 陈志德, 等. 江苏省扬中杂草稻生物学特性初步研究. 杂草科学, 2007(3): 13-152. [11] Hirai A, Ishibash T, Marlkami A, et al.Rice chloroplast DNA: A physical map and the location at the genes for the large subunit of ribulose 1,5bisphosphate carboxylase and the 32 kD photosystem Ⅱ reaction center protein. Theor Appl Genet, 1985, 70: 117-122.[12]Hiratsuka J, Shimada H, Whittier R, et al.The complete sequence of the rice (Oryza sativa) chloroplast genome: Intermolecular recombination between distinct tRNA genes accounts for a maior plastid DNA inversion during the evolution of the cereals. Mol Gen Genet, 1989, 21(7): 185-194.[13]Ishii T, Terachi T, Tsunewaki K.Restriction endonuchiase analysis of chloroplast DNA from cultivated rice species, Oryza sativa and O.glaberrima. Jpn J Genet, 1986, 61: 537-541.[14]Ishii T, Terachi T, Tsunewaki K. Restriction endonuclease analysis of chloroplast DNA from a genome diploid species of rice. Jpn J Genet, 1988, 63: 523-536.[15]Kanno A, Watanabe N, Nakamura I, et al. Variation in chloroplast DNA from rice(Oryza sativa): Differences between deletions mediated by short direct repeat sequences within a single species. Theor Appl Genet, 1993, 86: 579-584.[16]Chen W B, Nakamura I, Sato Y I, et al. Distribution of deletion type in cpDNA of cultivated and wild rice. Jpn J Genet, 1993, 68: 597603.[17]Chen W B, Nakamura I, Sato Y I, et al. Indicajaponica differentiation in Chinese rice landraces. Euphytica, 1994, 74: 195-201.[18]Sato Y I. Variation in spikelet shape of the indica and japonica rice cultivars in Asian origin.Jpn J Breed, 1991, 41: 121-134.[19]Sun C Q, Wang X K, Yoshimura A, et al. Genetic differentiation or nuclear, mitochondrial and chloroplast genomes in common wild rice (Oryza rufipogon Griff.) and cultivated rice (Oryza sativa L.). Theor Appl Genet, 2002, 104: 1335-1345.[20]Tang J B, Xia H A, Cao M L, et al. A comparison of rice chloroplast genomes. Plant Physiol, 2004, 135: 412-420. [21]江苏省农科院粮食所品种资源室.连云港地区穭稻初步考查. 江苏农业科学, 1984(3): 46.[22]Chen J J, Ding J H, Ouyang Y D, et al. A triallelic system of S5 is a major regulator of the reproductive barrier and compatibility of indicajaponica hybrids in rice. Proc Natl Acad Sci USA, 2008, 105(32): 11436-11441.[23]Dally A M, Second G.Chloroplast DNA diversity in wild and cultivated species of rice(Genus Oryza, Section Oryza): Cladisticmutation and geneticdistance analysis.Theor Appl Genet, 1990, 80: 209-222.[24]Andersen J R, Lübberstedt T. Functional markers in plants. Trends Plant Sci, 2003, 8(11): 554-560.[25] 蒋菏, 吴竞仑, 王根来. 连云港穭稻研究. 作物品种资源, 1985(2): 4-7.[26]魏兴华, 杨致荣, 董岚, 等. 穭稻分类地位的SSR证据. 中国农业科学, 2004, 37(7): 937-942.[27]Zhu S S, Jiang L, Wang C M, et al. The origin of weedy rice Ludao in China deduced by genome wide analysis of its hybrid sterility genes. Breeding Sci, 2005, 55(4): 409-414.[28]Jing W, Zhang W, Jiang L, et al. Two novel loci for pollen sterility in hybrids between the weedy strain Ludao and the japonica variety Akihikari of rice (Oryza sativa L.). Theor Appl Genet, 2007, 114: 915-925.[29]仲维功, 杨杰, 陈志德, 等. 江苏扬中杂草稻的籼粳分类. 江苏农业学报, 2006, 22(3): 238-242.[30]许聪, 吴万春. 海南岛杂草稻的生态考察和鉴定. 中国水稻科学, 1996, 10(4): 247-249.
[1] 杨杰 ,曹卿 ,王军 ,范方军,张玉琼,仲维功, . 水稻多酚氧化酶基因功能标记的开发与应用 [J]. 中国水稻科学, 2011, 25(1): 37-42 .
[2] 左娇,强胜,宋小玲. 温室条件下抗除草剂转基因水稻与杂草稻杂交和回交后代的适合度分析[J]. 中国水稻科学, 2010, 24(6): 608-616 .
[3] 江云珠,汤圣祥,唐健. 中国栽培稻等位酶的遗传结构及地理分布[J]. 中国水稻科学, 2010, 24(5): 453-462 .
[4] 杨 琳,戴伟民,强 胜,宋小玲. 杂草稻和栽培稻叶片下表皮结构特征的观察及聚类分析[J]. 中国水稻科学, 2009, 23(5): 495-502 .
[5] 傅雪琳,卢永根,刘向东,李金泉,赵杏娟. 不同倍性栽培稻与药用野生稻种间杂交不结实性的比较胚胎学观察[J]. 中国水稻科学, 2008, 22(4): 385-391 .
[6] 练子贤,魏常敏,卢永根,赵杏娟 ,傅雪琳 ,李金泉,杨秉耀 ,刘向东,. 广东高州普通野生稻与粳稻杂交F1胚囊育性及发育特点[J]. 中国水稻科学, 2008, 22(3): 266-272 .
[7] 马殿荣,王楠,王莹,贾德涛,陈温福. 中国北方杂草稻深覆土条件下出苗动力源分析[J]. 中国水稻科学, 2008, 22(2): 215-218 .
[8] 傅雪琳,卢永根,刘向东,李金泉. 利用种间杂交途径向栽培稻转移非AA组野生稻有利基因的研究进展[J]. 中国水稻科学, 2007, 21(6): 559-566 .
[9] 程桂平,冯九焕,梁国华,刘向东,李金泉,. 栽培稻与普通野生稻BC2F2群体产量相关性状的QTL分析[J]. 中国水稻科学, 2006, 20(5): 553-556 .
[10] 邵国胜,谢志奎,张国平,. 杂草稻和栽培稻氮代谢对镉胁迫反应的差异[J]. 中国水稻科学, 2006, 20(2): 189-193 .
[11] 李亚莉,杨晓曦,赵丰萍, 许明辉,. 云南元江普通野生稻(Oryza rufipogon)群体籼粳分化的SSR分析[J]. 中国水稻科学, 2006, 20(2): 137-140 .
[12] 徐鹏, 陶大云, 胡凤益, 周家武, 李静, 邓先能. 栽培稻种间杂交改良云南粳稻品种研究[J]. 中国水稻科学, 2005, 19(1): 41-46 .
[13] 陈惠哲, 玄松南, 王渭霞, 邵国胜, 孙宗修. 丹东杂草稻种子的耐冻能力和低温发芽特性研究[J]. 中国水稻科学, 2004, 18(2): 109-112 .
[14] 魏兴华, 汤圣祥, 江云珠, 余汉勇, 裘宗恩, 颜启传. 中国栽培稻选育品种等位酶多样性及其与形态学性状的[J]. 中国水稻科学, 2003, 17(2): 123-128 .
[15] 陶大云, 徐鹏, 李静, 杨友琼, 周家武, 胡凤益, Monty P. JONES ,. 栽培稻种间近等基因系杂种育性遗传及其基因定位[J]. 中国水稻科学, 2003, 17(1): 11-15 .
[16] 余柳青, 徐正浩, 郭怡卿, 陶大云. 野生稻和非洲栽培稻抗稗草作用研究初报[J]. 中国水稻科学, 2002, 16(3): 288-290 .
[17] 陶大云 , 徐 鹏 , 胡凤益 , 杨友琼 , 李 静 , 周家武 . 栽培稻种间近等基因系杂种育性研究[J]. 中国水稻科学, 2002, 16(2): 106-110 .
[18] 程式华, 毛传澡, 占小登, 斯华敏, 孙宗修. 籼粳交DH群体和RIL群体的构建及籼粳分化[J]. 中国水稻科学, 2001, 15(4): 257-260 .
[19] 罗利军, 张启发. 栽培稻抗旱性研究的现状与策略[J]. 中国水稻科学, 2001, 15(3): 209-214 .
[20] 赵笃乐,裴安平,张文绪. 湖南澧县八十垱遗址古栽培稻的再研究[J]. 中国水稻科学, 2000, 14(3): 139-143 .
[21] 刘国庆 ,颜辉煌 , 罗耀武 ,闵绍楷 ,朱立煌 ,. 栽培稻与紧穗野生稻间整倍体后代的RFLP分析[J]. 中国水稻科学, 1999, 13(3): 129-133 .
[22] 黄艳兰,张乃群,何光存 ,舒理慧,廖兰杰,祝莉莉. 我国三种野生稻及其与栽培稻种间杂种外稃表面显微特征的比较观察[J]. 中国水稻科学, 1999, 13(2): 77-80 .
[23] 许 聪 ,吴万春 . 海南岛杂草稻的生态考察和鉴定[J]. 中国水稻科学, 1996, 10(4): 247-249 .
[24] 颜辉煌,胡慧英,傅 强,余汉勇,汤圣祥,熊振民,闵绍楷. 栽培稻与药用野生稻杂种后代的形态学和细胞遗传学研究[J]. 中国水稻科学, 1996, 10(3): 138-142 .
[25] 肖 晗,应存山,黄大年. 中国栽培稻及其近缘野生种叶绿体DNA的限制性片段长度多态性分析[J]. 中国水稻科学, 1996, 10(2): 121-124 .
[26] 汤圣祥, 张文绪. 三种原产中国的野生稻和栽培稻外稃表面乳突结构的比较观察研究[J]. 中国水稻科学, 1996, 10(1): 19-22 .
[27] 孙传清, 毛龙, 王振山, 朱立煌, 王象坤. 栽培稻和普通野生稻基因组的随机扩增多态性DNA(RAPD)初步分析[J]. 中国水稻科学, 1995, 9(1): 1-6 .
[28] 王象坤, 才宏伟,孙传清,王振山,庞汉华. 中国普通野生生稻的原始型及其是否存在籼粳分化的初探[J]. 中国水稻科学, 1994, 8(4): 205-210 .
[29] 湛小燕,林榕辉. 部分栽培稻和野生稻种子谷蛋白的电泳分析[J]. 中国水稻科学, 1991, 5(3): 109-113 .
[30] 卢永根,万常炤,张桂权. 我国三个野生稻种粗线期核型的研究[J]. 中国水稻科学, 1990, 4(3): 97-105 .
[31] 罗利军,应存山,王一平. 光敏感核不育系在水稻广亲和性种质筛选中的应用[J]. 中国水稻科学, 1990, 4(3): 143-144 .
[32] 张桂权, 卢永根. 栽培稻(Oryza sativa)杂种不育性的遗传研究: Ⅰ.等基因F1不育系杂种不育性的双列分析[J]. 中国水稻科学, 1989, 3(3): 97-101 .
[33] 汤陵华, 佐藤洋一郎, 森岛启子. 亚洲栽培稻两大亚种之间同工酶基因型的主要区别[J]. 中国水稻科学, 1989, 3(3): 141-144 .
[34] 周汇, JC Glaszmann, 程侃声, 施晓群. 栽培稻分类方法的比较[J]. 中国水稻科学, 1988, 2(1): 1-7 .
版权所有 © 《中国水稻科学》编辑部 浙ICP备05004719号-5
地 址:浙江省杭州市体育场路359号   邮 编:310006   电 话:0571-63370278   E-mail:cjrs@263.net
本系统由北京玛格泰克科技发展有限公司设计开发  技术支持:support@magtech.com.cn