中国水稻科学

中国水稻科学 ›› 2012, Vol. 26 ›› Issue (5): 583-591.DOI: 10.3969/j.issn.10017216.2012.05.011

• 研究报告 • 上一篇    下一篇

连续交叉接种对云南高原粳稻白叶枯病菌株变异的影响

杨雅云1,3,4,# ,彭新禧1,2,#,徐福荣1,3,4,和建平1,2,阿新祥1,3,4,董超1,3,4,张恩来1,3,4,张斐斐1,3,4 ,汤翠凤1,3,4,戴陆园1,3,4,*    

  1. 1云南省农业科学院 生物技术与种质资源研究所， 云南 昆明 650205； 2云南大学 生命科学学院，  云南 昆明 650091； 3云南省农业生物技术重点实验室,  云南 昆明 650223； 4 农业部西南作物基因资源与种质创制重点实验室,  云南 昆明 650223；
  • 收稿日期:2011-08-23 修回日期:2011-11-12 出版日期:2012-09-10 发布日期:2012-09-10
  • 通讯作者: 戴陆园1,3,4,*
  • 基金资助:

    农业部948计划资助项目(2006G1)；云南省自然科学基金资助项目(2007C233M)。

Impact of Continuous Cross Inoculation on Variation of Xanthomonas oryzae pv.   oryzae from japonica Rice in Yunnan Plateau

YANG  Yayun 1，3,4,# , PENG Xinxi 1,2，# , XU  Furong 1,3,4 , HE Jianping 1,2 , A Xinxiang 1,3,4 , DONG Chao 1,3,4 , ZHANG Enlai 1,3,4 ,  ZHANG Feifei 1，3,4 , TANG Cuifeng 1,3,4 , DAI  Luyuan 1,3,4,*   

  1. 1 Institute of Biotechnology and Germplasm Resources, Yunnan Academy of Agricultural Sciences, Kunming 650205, China; 2 School of Life Sciences of Yunnan University, Kunming 650091, China;3 Yunnan Provincial Key Laboratory  of Agricultural Biotechnology；4 Key Laboratory of Southwestern Crop Gene Resource and Germplasm Innovation, Ministry of Agriculture； #These authors contributed equally to this paper；
  • Received:2011-08-23 Revised:2011-11-12 Online:2012-09-10 Published:2012-09-10
  • Contact: DAI Luyuan1,3,4,*

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摘要： 为了探索影响高原粳稻区白叶枯病菌变异的因子，以2个云南高原粳稻区白叶枯菌株20012、K1为供试菌株，在毫糯扬、金南风、滇粳优5号和合系41等不同品种组合上连续接种或连续交叉接种后分离菌株，利用高原粳稻白叶枯病菌的鉴别品种和分子检测手段，对获得的菌株的致病型及基因变异进行分析。结果显示，原始菌株20012与其7个获得菌系（23个菌株）的致病型都不同，其中，除获得菌系62的3个菌株致病力增强外，其他6个获得菌系的20个菌株致病力均减弱；而原始菌株K1的抗感反应模式与其获得菌系68的7个菌株完全相同。利用IS1112、hrpF引物进行菌株PCR扩增以及UPGMA聚类分析并将相应序列测序，结果显示，供试菌株IS1112、hrpF分子谱型的遗传分簇与菌株致病型之间无一一对应关系；在基因序列上，各菌株的IS1112和hrpF的两个序列片段表现出不同的碱基变化特征。两个原始菌株接种在不同抗性品种组合上获得的菌系的致病性变异具有特殊性和不定向性。

关键词: 云南高原, 白叶枯菌, 交叉接种, 变异

Abstract: To  study the  factors   influenceing  variation of Xanthomonas oryzae pv. oryzae( Xoo) from japonica rice in Yunnan plateau, eight   strains(30 isolates)   derived from two original strains 20012 and K1  were obtained through successivecross inoculation.Their pathotype and gene variation were studied by using differential cultivars and molecular detection methods. The results showed that  seven obtained strains including 23 isolates observably differed with the  original isolate 20012 in pathotype, beside the pathogenicity of 62 containing 3 isolates were increased, while the pathogenicity of  the other six strains containing 20 isolates were decreased. The pathotypes of 68 were the  same as that of its original isolate K1. By using UPGMA analysis of PCR producets of primer  IS1112  and  hrpF, no   relationship between molecular haplotypes and pathotypes were found. Sequence analysis showed that basic group variation  in sequences of all strains amplified by primers  IS1112  and  hrpF differed. The pathogenic variation of obtained strains from two original strains  inoculated on various varieties were unique and nondirective.

Key words: Yunnan plateau, Xanthomonas oryzae pv.oryzae, continuous cross inoculation, variation

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