中国水稻科学

中国水稻科学

• 研究报告 • 上一篇    下一篇

水稻细菌性谷枯病病原菌的分离鉴定

罗金燕1; 徐福寿2;王平3;徐丽慧1;谢关林1,*   

  1. 1浙江大学 生物技术研究所， 浙江 杭州 310029； 2杭州市植物保护土肥总站, 浙江 杭州 310020; 3黑龙江省农垦科学院, 黑龙江 佳木斯 154007
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2008-01-10 发布日期:2008-01-10

Isolation and Identification of the Causal Organism of Bacterial Grain Rot from Rice

LUO Jin-yan , XU Fu-shou , WANG Ping , XU Li-hui , XIE Guan-lin   

  • Received:1900-01-01 Revised:1900-01-01 Online:2008-01-10 Published:2008-01-10
  • Contact: XIE Guan-lin

PDF

摘要： 经多年的病原菌分离监测, 于2006年从2份浙江省稻种(来自海南的健康籼稻)上分离到6株病原细菌，经25项主要生理生化特性、菌落形态、致病性、Biolog、脂肪酸分析和RAPDPCR鉴定，发现这种病原细菌为Burkholderia glumae， 是引起水稻细菌性谷枯病的病原菌。此外，实验证明“健康稻种”也可能存在水稻细菌性谷枯病菌。

关键词: 水稻细菌性谷枯病菌, 表型鉴定, 分子鉴定

Abstract: A survey on isolation and detection of the casual organism of bacterial grain rot from rice was conducted during 1997-2006. In 2006, six pathogenic bacterial strains were isolated from two asymptomatic rice seed samples (Oryza sativa L.) originally produced in Hainan Province and then planted in Zhejiang Province. They were identified as Burkholderia glumae, which is the causal organism of bacterial grain rot of rice, by physiological characteristics, colony morphology, pathogenicity test, Biolog, fatty acid methyl ester (FAME) analysis and RAPDPCR compared with the four standard reference strains. It is confirmed that there exsits Burkholderia glumae in socalled “health looking seeds”.

Key words: Burkholderia glumae, phenotypical identification, molecular identification, pathogenicity