利用Wx和fgr基因双功能性标记高效选育优质水稻保持系

doi:10.3969/j.issn.1001-7216.2011.01.005

中国水稻科学

利用Wx和fgr基因双功能性标记高效选育优质水稻保持系

陈深广¹;周屹峰²;赵霏²;金亮²;沈圣泉^2,*

¹中国水稻研究所，浙江杭州310006； ²浙江大学农业与生物技术学院，浙江杭州 310029； *通讯联系人， E-mail: shenshq@zju.edu.cn

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2011-01-10 发布日期:2011-01-10

Efficient Selection of Fine Rice Quality Maintainer Lines with Functional Markers of Wx and fgr Genes

CHEN Shen-guang¹; ZHOU Yi-feng²;ZHAO Fei²; JIN Liang²;SHEN Sheng-quan^{2 ,*}

¹China National Rice Research Institute, Hangzhou 310006, China; ²College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China; *Corresponding author, E-mail: <A href=

Received:1900-01-01 Revised:1900-01-01 Online:2011-01-10 Published:2011-01-10

摘要/Abstract

摘要： 对选取的13份籼型保持系进行直链淀粉含量和香味测定及分子标记（484/485和fgr1F/1R）检测，筛选出宜香1B作为试验用分子育种优质供体亲本。配组Ⅱ32B/宜香1B，F2代进行该双标记分离分析，结果表明，不仅F2单株间特异性谱带差别明显便于鉴别，且呈现出两对独立遗传基因分离模式。选取F2代 484/485和fgr1F/1R呈Ⅰ型（与宜香B相同的带型）和Ⅲ型（杂合带型）的优良单株，经F3~F6加代，并结合农艺性状（株型、播抽历期等）和稻米品质性状（粒型、垩白度、透明度等）鉴定和选择，在F6再利用484/485和fgr1F/1R分子标记进行复测和验证，从而获得了一批株系内性状基本稳定、株型好、直链淀粉含量中等、具有香味、外观和蒸煮食用品质优良的高世代株系。经测交鉴定，发现其保持系株系比例约占75.5%。表明利用Wx和fgr基因功能性分子标记的早世代检测和辅助选择，结合常规优质保持系鉴定和选育方法，可以快速获得直链淀粉含量中等、香味、长粒型、透明、低垩白、较易糊化等综合性状较优的保持系。

关键词: 籼稻, 分子标记辅助选择, 直链淀粉含量, 香味, 功能性标记

Abstract: According to the genotypes detected by two primers of functional markers, 484/485 (for Wx gene) and fgr1F/1R(for fgr gene), amylose contents and fragrance of 13 indica maintainer lines, Yixiang 1B was selected as good rice quality donor parent. The two markers were used to genotype the F2 individual plants derived from Ⅱ32B/Yixiang 1B. There were significant difference in electrophoretogram among individual plants, so that they were easy to distinguish, and these two markers showed independent inheritance. Superior individuals with fgr and Wx genotypeⅠ(same as Yixiang 1B) and Ⅲ (heterozygote) were selected. Then combined with the selection of agronomic and rice quality characters, the detection in selected plants or individual plants in F3 to F6 generations were carried out. In F6 generation, the plants were detected by 484/485 and fgr1F/1R again, advanced plants were obtained, which had stable characters, good plant type, obvious fragrance, medium amylose content and so on. Results of testcross showed that 75.5% plants were maintainer lines. It is suggested that detection and selection of maintainer lines of indica rice with functional markers of Wx and fgr genes in earlygeneration combined with conventional breeding could provide a rapid way to obtain good rice quality maintainer lines with fragrance, medium amylose content, slender grain shape, low chalkiness degree, high transparency and so on.

Key words: indica rice, markerassisted selection, amylose content, fragrance, functional marker

陈深广,周屹峰,赵霏,金亮,沈圣泉,. 利用Wx和fgr基因双功能性标记高效选育优质水稻保持系[J]. 中国水稻科学 2011,25(1): 31-36 . , DOI: 10.3969/j.issn.1001-7216.2011.01.005 .

CHEN Shen-guang,ZHOU Yi-feng,ZHAO Fei,JIN Liang,SHEN Sheng-quan . Efficient Selection of Fine Rice Quality Maintainer Lines with Functional Markers of Wx and fgr Genes [J]. Chinese Journal of Rice Science 2011,25(1): 31-36 ., DOI: 10.3969/j.issn.1001-7216.2011.01.005 .

参考文献

[1]朱昌兰, 沈文飚, 翟虎渠, 等. 水稻低直链淀粉含量基因育种利用的研究进展. 中国农业科学, 2004, 37（2）: 157-162.

[2]白德朗, 魏薇, 韦燕萍, 等. 杂交香稻研究进展与展望. 广西农业科学, 2008, 39(6): 717-724.

[3]Cordeiro G M, Christopher M J, Henry R J, et al. Identification of microsatellite markers for fragrance in rice by analysis of the rice genome sequence. Mol Breeding, 2002, 9(4): 245-250.

[4]Bligh H F J, Till R I, Jones C A. A microsatellite sequence closely linked to the waxy gene of Oryza sativa. Euphytica, 1995, 86(2): 83-85.

[5]Buttery R G, Ling L C, Juliano B O. 2-acetyl-1-pyrroline: An important aroma component of cooked rice. Chem Ind (Lond), 1982, 12: 958-959.

[6]Chen S H, Wu J, Yang Y, et al. The fgr gene responsible for rice fragrance was restricted within 69 kb. Plant Sci, 2006, 171: 505-514.

[7]Jin Q S, Waters D, Cordeiro G M, et al.A single nucleotide polymorphism (SNP) marker linked to the fragrance gene in rice (Oryza sativa L.). Plant Sci, 2003, 165: 359-364.

[8]Bradbury L M T, Fitzgerald T L, Henry R J, et al. The gene for fragrance in rice. Plant Biotech J, 2005, 3(3): 363-370.

[9]Wanchana S, Kamolsukyungyoung W, Ruengphayak S, et al. A rapid construction of a physical contig across a 4.5 cM region for rice grain aroma facilitates marker enrichment for positional cloning. Sci Asia, 2005, 31: 299-306.

[10]Chen M H, Bergman C, Pinson S, et al. Waxy gene haplotypes: Associations with apparent amylose content and the effect by the environment in an international rice germplasm collection. J Cereal Sci, 2008, 47: 536-545.

[11]Sun S H, Gao F Y, Lu X J, et al. Genetic analysis and gene fine mapping of aroma in rice (Oryza sativa L. Cyperales, Poaceae). Genet Mol Biol, 2008, 31: 532-538.

[12]金亮. 水稻关联定位群体的构建及若干品质性状的关联分析[D]. 杭州: 浙江大学, 2009,

[13]Perez C M, Juliano B O. Modification of the simplified amylose test for milled rice. Starch, 1978, 30: 424-426.

[14]中华人民共和国农业部部颁标准NY/T 83-1988. 米质测定方法. 北京: 中国标准测定出版社, 1988.

[15]Amarawathi Y, Singh R, Sing A K, et al. Mapping of quantitative trait loci for basmati quality traits in rice (Oryza sativa L.). Mol Breeding, 2008, 1(1): 49-65.

[16]王珍, 方宣钧. 植物DNA分离. 分子植物育种, 2003, 1(2): 281-288.

[17]Bradbury L M T, Gillies S A, Brushett D J, et al. Inactivation of an aminoaldehyde dehydrogenase is responsible for fragrance in rice. Plant Mol Biol, 2008, 68(4/5): 439-449.

[18]Bradbury L M T, Henry R J, Jin Q S, et al. A perfect marker for fragrance genotyping in rice. Mol Breeding, 2005, 16(4): 279-283.

[19]Andersen J R, Luebberstedt T. Functional markers in plants. Trends Plant Sci, 2003, 8(11): 554-560.

利用Wx和fgr基因双功能性标记高效选育优质水稻保持系

Efficient Selection of Fine Rice Quality Maintainer Lines with Functional Markers of Wx and fgr Genes

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

[1]	陈丽明, 杨陶陶, 熊若愚, 谭雪明, 黄山, 曾勇军, 潘晓华, 石庆华, 张俊, 曾研华. 开放式主动增温对双季优质籼稻籽粒淀粉积累及其关键酶活性的影响[J]. 中国水稻科学, 2023, 37(2): 166-177.
[2]	张佳, 王慧杰, 何正权, 刘文真. 农杆菌介导的籼稻9311和华占遗传转化体系的研究[J]. 中国水稻科学, 2023, 37(2): 213-224.
[3]	王石光, 陆展华, 刘维, 卢东柏, 王晓飞, 方志强, 巫浩翔, 何秀英. 应用CRISPR/Cas9技术与分子标记辅助选择创制广东丝苗米新种质[J]. 中国水稻科学, 2023, 37(1): 29-36.
[4]	陈涛, 赵庆勇, 朱镇, 赵凌, 姚姝, 周丽慧, 赵春芳, 张亚东, 王才林. 利用分子标记辅助选择培育优良食味、低谷蛋白香粳稻新品系[J]. 中国水稻科学, 2023, 37(1): 55-65.
[5]	毛慧, 彭彦, 毛毕刚, 韶也, 郑文杰, 胡黎明, 周凯, 赵炳然. 水稻直链淀粉合成调控新基因Wx410的功能与效应分析[J]. 中国水稻科学, 2022, 36(6): 579-585.
[6]	史玉良, 杨勇, 李雪飞, 李钱峰, 黄李春, 张昌泉, 宋学堂, 刘巧泉. 不同直链淀粉含量软米品种品质性状的比较[J]. 中国水稻科学, 2022, 36(6): 601-610.
[7]	张宇杰, 王志强, 马鹏, 杨志远, 孙永健, 马均. 麦秆还田下水氮耦合对水稻氮素吸收利用及产量的影响[J]. 中国水稻科学, 2022, 36(4): 388-398.
[8]	魏晓东, 张亚东, 赵凌, 路凯, 宋雪梅, 王才林. 稻米香味物质2-乙酰-1-吡咯啉的形成及其影响因素[J]. 中国水稻科学, 2022, 36(2): 131-138.
[9]	董铮, 王雅美, 黎用朝, 熊海波, 薛灿辉, 潘孝武, 刘文强, 魏秀彩, 李小湘. 基于MAGIC群体的水稻镉含量全基因组关联分析[J]. 中国水稻科学, 2022, 36(1): 35-42.
[10]	王文霞, 陈丽明, 王海霞, 刘有清, 吴自明, 曾勇军, 谭雪明, 潘晓华, 石庆华, 曾研华. 淹水缓解直播早籼稻苗期低温冷害的生理特性研究[J]. 中国水稻科学, 2021, 35(2): 166-176.
[11]	王志东, 陈宜波, 龚蓉, 周少川, 王重荣, 李宏, 黄道强, 周德贵, 赵雷, 潘阳阳, 杨义强, 李晓芳. 优质籼稻剑叶SPAD值与稻米品质相关性研究[J]. 中国水稻科学, 2021, 35(1): 89-97.
[12]	王文霞, 周燕芝, 曾勇军, 吴自明, 谭雪明, 潘晓华, 石庆华, 曾研华. 不同机直播方式对南方优质晚籼稻产量及抗倒伏特性的影响[J]. 中国水稻科学, 2020, 34(1): 46-56.
[13]	龙起樟, 黄永兰, 唐秀英, 王会民, 芦明, 袁林峰, 万建林. 利用CRISPR/Cas9敲除OsNramp5基因创制低镉籼稻[J]. 中国水稻科学, 2019, 33(5): 407-420.
[14]	郝中娜, 毛雪琴, 柴荣耀, 王艳丽, 孙国昌. 国家长江中下游稻区品种区域试验籼稻稻瘟病抗性分析[J]. 中国水稻科学, 2019, 33(2): 152-157.
[15]	张宏根, 仲崇元, 司华, 刘巧泉, 顾铭洪, 汤述翥. 分子标记辅助选择改良C418对红莲型粳稻不育系的恢复力[J]. 中国水稻科学, 2018, 32(5): 445-452.