中国水稻科学

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水稻条纹病毒NS3蛋白与水稻 3-磷酸甘油醛脱氢酶(GAPDH)

肖冬来;贾东升;吴建国;杜振国;谢荔岩;吴祖建*;谢联辉*   

  1. 福建农林大学 植物病毒研究所/福建省植物病毒学重点实验室, 福建 福州 350002; *通讯联系人, E-mail: wuzujian@126.com; fjxlh@126.com
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-09-10 发布日期:2010-09-10

Interaction Between Rice Stripe Virus NS3 Protein and Glyceraldehyde-3-Phosphate Dehydrogenase(GAPDH) of Rice

XIAO Dong-lai;JIA Dong-sheng;WU Jian-guo; DU Zhen-guo;XIE Li-yan; WU Zu-jian*; XIE Lian-hui*   

  1. Key Laboratory of Plant Virology of Fujian Province/Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou 350002, China; *Corresponding author, E-mail: wuzujian@126.com; fjxlh@126.com
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-09-10 Published:2010-09-10

摘要: 水稻条纹病毒(rice stripe virus,RSV)NS3蛋白为病毒的沉默抑制子。利用酵母双杂交技术,从水稻cDNA文库中筛选出一个与RSV NS3蛋白互作的基因片段。推测该基因的功能是编码水稻3磷酸甘油醛脱氢酶(glyceraldehyde-3-phosphate dehydrogenase,GAPDH)。双分子荧光互补实验进一步验证了NS3与GAPDH存在互作。瞬时表达实验表明,GAPDH与GFP基因融合蛋白在本氏烟表皮细胞细胞质中大量积累。此外,讨论了GAPDH蛋白在RSV侵染水稻过程中可能的功能。

关键词: 水稻条纹病毒, 蛋白功能, 互作, 酵母双杂交, 双分子荧光互补

Abstract: NS3 protein of rice stripe virus (RSV) is an RNA silencing suppressor. Yeast twohybrid assay was used to screen the rice cDNA library with the NS3 protein as a bait. A novel rice protein interacting with RSV NS3 was obtained. The putative function of the interacting protein was glyceraldehyde3phosphate dehydrogenase (GAPDH). Bimolecular fluorescence complementation (BiFC) also showed that RSV NS3 could interact with the GAPDH. Cellular localization studies showed that GAPDHGFP fusion protein accumulated predominantly in cytoplasm of tobacco (Nicotiana benthamiana) through transient expression assay. Possible functions of GAPDH during the infection of rice by RSV were discussed.

Key words: rice stripe virus, protein function, interaction, yeast two hybrid, bimolecular fluorescence complementation