Rice Blast Resistance of Transgenic Rice Plants with Pi-d2 Gene
CHEN De-xi1,2， CHEN Xue-wei1， LEI Cai-lin3， MA Bing-tian1， WANG Yu-ping1， LI Shi-gui1，*
1Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, China; 2Institute of Plant Protection, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China； 3Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China; *Corresponding author, E-mail: lishigui_sc@263.net)
Abstract：
Transgenic rice lines harboring Pid2 gene under the control of different promoters from three expression vector pCB6.3kb, pCB53kb and pZH01272kb were tested for resistance to Magnaporthe grisea. The results showed that 9 stably transgenic lines had different resistances to 39 strains of M. grisea from Sichuan Province, China, and the highest diseaseresistance frequency was 917%. Four early homozygous transgenic lines of Pid2 gene exhibited resistance to more than 8148% of 58 strains from Chinese Academy of Agricultural Sciences, which indicates that the transgenic rice lines possessed widespectrum resistance against various rice blast races. The results of transgenic embryonic callus selected by crude toxin showed that the induction rate of embryonic callus reduced by increased concentration of toxin in medium. When the toxin concentration was 40% in medium, the induction rate of embryonic callus of transgenic plant was 4933%, while that of the receptor control was 5%. The disease incidence of neck blast were 0% to 50% in the transgenic lines under induction of rice blast disease in fields, showing the higher resistance to rice blast than that of receptor control.
CHEN De-xi,CHEN Xue-wei,LEI Cai-lin et al. Rice Blast Resistance of Transgenic Rice Plants with Pi-d2 Gene [J]. , 2010, 24(1): 31-35 .
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