Rice Blast Resistance of Transgenic Rice Plants with Pi-d2 Gene
CHEN De-xi1,2, CHEN Xue-wei1, LEI Cai-lin3, MA Bing-tian1, WANG Yu-ping1, LI Shi-gui1,*
1Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, China; 2Institute of Plant Protection, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China; 3Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China; *Corresponding author, E-mail: lishigui_sc@263.net)
Abstract:
Transgenic rice lines harboring Pid2 gene under the control of different promoters from three expression vector pCB6.3kb, pCB53kb and pZH01272kb were tested for resistance to Magnaporthe grisea. The results showed that 9 stably transgenic lines had different resistances to 39 strains of M. grisea from Sichuan Province, China, and the highest diseaseresistance frequency was 917%. Four early homozygous transgenic lines of Pid2 gene exhibited resistance to more than 8148% of 58 strains from Chinese Academy of Agricultural Sciences, which indicates that the transgenic rice lines possessed widespectrum resistance against various rice blast races. The results of transgenic embryonic callus selected by crude toxin showed that the induction rate of embryonic callus reduced by increased concentration of toxin in medium. When the toxin concentration was 40% in medium, the induction rate of embryonic callus of transgenic plant was 4933%, while that of the receptor control was 5%. The disease incidence of neck blast were 0% to 50% in the transgenic lines under induction of rice blast disease in fields, showing the higher resistance to rice blast than that of receptor control.
CHEN De-xi,CHEN Xue-wei,LEI Cai-lin et al. Rice Blast Resistance of Transgenic Rice Plants with Pi-d2 Gene [J]. , 2010, 24(1): 31-35 .
[1]
Qu S H. Rice diseases. 2nd edn. Kew, Surrey:Commonwealth Mycological Institute, CABI, 1985: 109-201.
[2]
Nishizawa Y, Nishio Z, Nakazono K, et al. Enhanced resistance to blast (Magnaporthe grisea) in transgenic japonica rice by constitutive expression of rice chitinase.Theor Appl Genet, 1999, 99: 383-390.
[3]
Starklorenzen P, Nelke B, Han G, et al. Transfer of a grapevine synthase gene to rice (Oryza sativa L.). Plant Cell Rep, 1997, 16: 668-673.
[4]
Feng D R, Xu X P, Wei J W, et al. Enhancement of rice disease resistance by two antifungal protein genes.Acta Bot Sin, 1999, 41(11): 1187-1191.
[5]
Ming X T, Wang L J, An C C, et al. Resistance to rice blast (Pyricularia oryzae) caused by the expression of trichosanthin gene in transgenic rice plants transferred through Agrobacterium method. Chin Sci Bull, 2000, 19: 1774-1778.
[6]
Kanzaki H, Nirasawa S, Saitoh H, et al. Overexpression of the wasabi defensin gene confers enhanced resistance to blast fungus(Magnaporthe grisea) in transgenic rice. Theor Appl Genet, 2002, 105: 809-814.
[7]
Wang Z X, Yano M, Yamanouchi U, et al. The Pib gene for rice blast resistance belongs to the nucleotide binding and leucinerich repeat class of plant disease resistance genes. Plant J, 1999, 19: 55-64.
[8]
Bryan G T, Wu K S, Farrall L, et al. A single amino acid difference distinguishes resistant and susceptible alleles of the rice blast resistance gene Pita. Plant Cell, 2000, 12: 2033-2045.
[9]
Qu S H, Liu G F, Zhou B, et al. The broadspectrum blast resistance gene Pi9 encodes an NBSLRR protein and is a member of a multigene family in rice. Genetics, 2006, 172: 1901-1914.
[10]
Zhou B, Qu S H, Liu G F, et al. The eight amino acid differences within three leucinerich repeats between Pi2 and Pizt resistance proteins determine the resistance specificity to Magnaporthe grisea. Mol PlantMicrobe Interact, 2006, 19: 1216-1228.
[11]
Chen X W, Shang J J, Chen D X, et al. A Blectin receptor kinase gene conferring rice blast resistance. Plant J, 2006, 46: 794-804.
[12]
Lin F, Chen S, Que Z Q, et al. Molecular cloning and characterization of the rice blast resistance gene Pi37 in the wellknown cultivar St. No. 1//Abstracts of the 4th International Rice Blast Conference. Changsha, China, October 9 14, 2007. Changsha:[s.n.], 2007.
Cao M X, Huang J Q, Wei Z M, et al. Agrobacteriummediated multiple gene transformation in rice using a single vector. J Integr Plant Biol, 2005, 47(2): 233-242.