转Pi-d2基因水稻对稻瘟病的抗性分析

doi:10.3969/j.issn.1001-7216.2010.01.06

中国水稻科学

转Pi-d2基因水稻对稻瘟病的抗性分析

陈德西1，2;陈学伟1;雷财林3; 马炳田1;王玉平1;李仕贵1，*

1四川农业大学水稻研究所，四川成都 611130； 2四川省农业科学院植物保护研究所，四川成都 610066； 3中国农业科学院作物科学研究所，北京100081; *通讯联系人， E-mail: lishigui_sc@263.net

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-01-10 发布日期:2010-01-10

Rice Blast Resistance of Transgenic Rice Plants with Pi-d2 Gene

CHEN De-xi1,2， CHEN Xue-wei1， LEI Cai-lin3， MA Bing-tian1， WANG Yu-ping1， LI Shi-gui1，*

1Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, China; 2Institute of Plant Protection, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China； 3Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China; *Corresponding author, E-mail: lishigui_sc@263.net)

Received:1900-01-01 Revised:1900-01-01 Online:2010-01-10 Published:2010-01-10

摘要/Abstract

摘要： 对由pCB63kb、pCB53kb和pZH01272kb三个不同表达载体转化的转稻瘟病抗性基因Pid2水稻株系进行稻瘟病抗性分析。结果表明，转Pid2基因水稻的9个高代株系对来自四川的39个稻瘟病菌株表现不同的抗性，抗病频率最高达917%；4个转基因早代纯合株系对来自中国农业科学院的58个菌株中的8148%以上菌株表现抗性，具有广谱抗性特点。稻瘟病菌粗毒素筛选结果表明，来自转基因植株的幼胚愈伤组织诱导率随培养基中粗毒素浓度提高而降低，粗毒素浓度达到40%时，幼胚愈伤诱导率为4933%，受体对照幼胚愈伤组织诱导率为5%。田间诱发条件下，转基因株系在大田的穗瘟发病率为0%~50%, 抗性较受体对照大幅度提高。

关键词: 转基因水稻, 抗病基因, 稻瘟病, 抗谱, 粗毒素

Abstract: Transgenic rice lines harboring Pid2 gene under the control of different promoters from three expression vector pCB6.3kb, pCB53kb and pZH01272kb were tested for resistance to Magnaporthe grisea. The results showed that 9 stably transgenic lines had different resistances to 39 strains of M. grisea from Sichuan Province, China, and the highest diseaseresistance frequency was 917%. Four early homozygous transgenic lines of Pid2 gene exhibited resistance to more than 8148% of 58 strains from Chinese Academy of Agricultural Sciences, which indicates that the transgenic rice lines possessed widespectrum resistance against various rice blast races. The results of transgenic embryonic callus selected by crude toxin showed that the induction rate of embryonic callus reduced by increased concentration of toxin in medium. When the toxin concentration was 40% in medium, the induction rate of embryonic callus of transgenic plant was 4933%, while that of the receptor control was 5%. The disease incidence of neck blast were 0% to 50% in the transgenic lines under induction of rice blast disease in fields, showing the higher resistance to rice blast than that of receptor control.

Key words: transgenic rice, resistance gene, rice blast, resistance spectrum, crude toxin

陈德西,陈学伟,雷财林, 马炳田,王玉平,李仕贵,. 转Pi-d2基因水稻对稻瘟病的抗性分析[J]. 中国水稻科学 2010,24(1): 31-35 . , DOI: 10.3969/j.issn.1001-7216.2010.01.06 .

CHEN De-xi,CHEN Xue-wei,LEI Cai-lin,MA Bing-tian,WANG Yu-ping,LI Shi-gui . Rice Blast Resistance of Transgenic Rice Plants with Pi-d2 Gene [J]. Chinese Journal of Rice Science 2010,24(1): 31-35 ., DOI: 10.3969/j.issn.1001-7216.2010.01.06 .

参考文献

[1]Qu S H. Rice diseases. 2nd edn. Kew, Surrey：Commonwealth Mycological Institute, CABI, 1985： 109-201.

[2]Nishizawa Y, Nishio Z, Nakazono K, et al. Enhanced resistance to blast (Magnaporthe grisea) in transgenic japonica rice by constitutive expression of rice chitinase.Theor Appl Genet, 1999, 99: 383-390.

[3]Starklorenzen P, Nelke B, Han G, et al. Transfer of a grapevine synthase gene to rice (Oryza sativa L.). Plant Cell Rep, 1997, 16: 668-673.

[4]Feng D R, Xu X P, Wei J W, et al. Enhancement of rice disease resistance by two antifungal protein genes.Acta Bot Sin, 1999, 41(11): 1187-1191.

[5]Ming X T, Wang L J, An C C, et al. Resistance to rice blast (Pyricularia oryzae) caused by the expression of trichosanthin gene in transgenic rice plants transferred through Agrobacterium method. Chin Sci Bull, 2000, 19: 1774-1778.

[6]Kanzaki H, Nirasawa S, Saitoh H, et al. Overexpression of the wasabi defensin gene confers enhanced resistance to blast fungus(Magnaporthe grisea) in transgenic rice. Theor Appl Genet, 2002, 105: 809-814.

[7]Wang Z X, Yano M, Yamanouchi U, et al. The Pib gene for rice blast resistance belongs to the nucleotide binding and leucinerich repeat class of plant disease resistance genes. Plant J, 1999, 19: 55-64.

[8]Bryan G T, Wu K S, Farrall L, et al. A single amino acid difference distinguishes resistant and susceptible alleles of the rice blast resistance gene Pita. Plant Cell, 2000, 12: 2033-2045.

[9]Qu S H, Liu G F, Zhou B, et al. The broadspectrum blast resistance gene Pi9 encodes an NBSLRR protein and is a member of a multigene family in rice. Genetics, 2006, 172: 1901-1914.

[10]Zhou B, Qu S H, Liu G F, et al. The eight amino acid differences within three leucinerich repeats between Pi2 and Pizt resistance proteins determine the resistance specificity to Magnaporthe grisea. Mol PlantMicrobe Interact, 2006, 19: 1216-1228.

[11] Chen X W, Shang J J, Chen D X, et al. A Blectin receptor kinase gene conferring rice blast resistance. Plant J, 2006, 46: 794-804.

[12] Lin F, Chen S, Que Z Q, et al. Molecular cloning and characterization of the rice blast resistance gene Pi37 in the wellknown cultivar St. No. 1//Abstracts of the 4th International Rice Blast Conference. Changsha, China, October 9 14, 2007. Changsha:[s.n.], 2007.

[13]刘文萍, 刘丽艳, 吕晓波, 等. 稻瘟病菌粗毒素的制备及其致病力的测定. 黑龙江农业科学, 1997（6）：14-17.

[14]许明辉, 李成云, 李进斌，等. 转溶菌基因水稻稻瘟病抗谱分析. 中国农业科学, 2003, 36(4): 387-392.

[15] 许明辉，李成云，李进斌，等. 转几丁质酶葡聚糖基因水稻稻瘟病抗谱分析. 中国水稻科学， 2003， 17(4): 307-310.

[16]Cao M X, Huang J Q， Wei Z M, et al. Agrobacteriummediated multiple gene transformation in rice using a single vector. J Integr Plant Biol, 2005, 47(2): 233-242.

[17]陈红旗, 陈宗祥, 倪深，等. 利用分子标记技术聚合3个稻瘟病基因改良金23B的稻瘟病抗性. 中国水稻科学， 2008，12（1）： 23-27.

转Pi-d2基因水稻对稻瘟病的抗性分析

Rice Blast Resistance of Transgenic Rice Plants with Pi-d2 Gene

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

[1]	肖乐铨, 李雷, 戴伟民, 强胜, 宋小玲. 转cry2A/bar*基因水稻与杂草稻杂交后代的苗期生长特性[J]. 中国水稻科学, 2023, 37(4): 347-358.
[2]	李刚, 高清松, 李伟, 张雯霞, 王健, 程保山, 王迪, 高浩, 徐卫军, 陈红旗, 纪剑辉. 定向敲除SD1基因提高水稻的抗倒性和稻瘟病抗性[J]. 中国水稻科学, 2023, 37(4): 359-367.
[3]	王雨, 孙全翌, 杜海波, 许志文, 吴科霆, 尹力, 冯志明, 胡珂鸣, 陈宗祥, 左示敏. 利用抗稻瘟病基因Pigm和抗纹枯病数量性状基因qSB-9^TQ、qSB-11^HJX改良南粳9108的抗性[J]. 中国水稻科学, 2023, 37(2): 125-132.
[4]	周永林, 申小磊, 周立帅, 林巧霞, 王朝露, 陈静, 冯慧捷, 张振文, 陈晓婷, 鲁国东. OsLOX10正调控水稻对稻瘟病和白叶枯病的抗性[J]. 中国水稻科学, 2022, 36(4): 348-356.
[5]	曹煜东, 肖湘谊, 叶乃忠, 丁晓雯, 易晓璇, 刘金灵, 肖应辉. 生长素调控因子OsGRF4协同调控水稻粒形和稻瘟病抗性[J]. 中国水稻科学, 2021, 35(6): 629-638.
[6]	刘树芳, 董丽英, 李迅东, 周伍民, 杨勤忠. 持有Pi9基因的水稻单基因系IRBL9-W对稻瘟病菌苗期和成株期抗性差异[J]. 中国水稻科学, 2021, 35(3): 303-310.
[7]	王小秋, 杜海波, 陈夕军, 李明友, 王嘉楠, 许志文, 冯志明, 陈宗祥, 左示敏. 江苏近年育成粳稻新品种/系的稻瘟病抗性基因及穗颈瘟抗性分析[J]. 中国水稻科学, 2020, 34(5): 413-424.
[8]	朱华珺, 周瑚, 任佐华, 刘二明. 枯草芽孢杆菌JN005胞外抗菌物质及对水稻叶瘟防治效果[J]. 中国水稻科学, 2020, 34(5): 470-478.
[9]	许赵蒙, 李利华, 高晓庆, 袁正杰, 李莘, 田旭丹, 王岚岚, 瞿绍洪. 转Pi9抗稻瘟病基因水稻株系的比较转录组分析[J]. 中国水稻科学, 2020, 34(3): 245-255.
[10]	孟峰, 张亚玲, 靳学慧. 黑龙江省稻瘟病菌无毒基因AVR-Pita及其同源基因的检测与分析[J]. 中国水稻科学, 2020, 34(2): 143-149.
[11]	李金璐, 张慧, 焦泽宇, 刘剑宇, 韩光煜, 卓晓轩, 罗琼. 水稻子预44和江南香糯基因组比较鉴定稻瘟病抗性相关基因[J]. 中国水稻科学, 2020, 34(1): 8-16.
[12]	陈涛, 孙旭超, 张善磊, 梁文化, 周丽慧, 赵庆勇, 姚姝, 赵凌, 赵春芳, 朱镇, 张亚东, 王才林. 稻瘟病广谱抗性基因Pigm特异性分子标记的开发和应用[J]. 中国水稻科学, 2020, 34(1): 28-36.
[13]	曹妮, 陈渊, 季芝娟, 曾宇翔, 杨长登, 梁燕. 水稻抗稻瘟病分子机制研究进展[J]. 中国水稻科学, 2019, 33(6): 489-498.
[14]	徐鹏, 王宏, 涂燃冉, 刘群恩, 吴玮勋, 傅秀民, 曹立勇, 沈希宏. 利用CRISPR/Cas9系统定向改良水稻稻瘟病抗性[J]. 中国水稻科学, 2019, 33(4): 313-322.
[15]	刘艳, 王宝祥, 杨波, 陈庭木, 邢运高, 孙志广, 徐波, 迟铭, 卢百关, 李健, 刘金波, 方兆伟, 秦德荣, 徐大勇. 黄淮海稻区水稻品种的穗颈瘟抗性[J]. 中国水稻科学, 2019, 33(4): 377-382.