中国水稻科学

中国水稻科学

• 研究报告 • 上一篇    下一篇

抗除草剂基因导入培矮64S实现杂交水稻制种机械化的初步研究

傅亚萍; 朱正歌; 肖 晗; 胡国成; 斯华敏; 于永红; 孙宗修   

  1. 中国水稻研究所, 农业部水稻生物学重点实验室，浙江 杭州 310006
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2001-04-10 发布日期:2001-04-10

Primary Study on Mechanization of Seed Production of Hybrid Rice by Inducing Bar Gene to Pei'ai 64S

FU Ya-ping; ZHU Zheng-ge; XIAO Han; HU Guo-cheng; SI Hua-min; YU Yong-hong; SUN Zong-xiu   

  1. (Key Laboratory for Rice Biology; Ministry of Agriculture; P.R.China; China National Rice Research Institute; Hangzhou 310006; China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2001-04-10 Published:2001-04-10

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被引次数

58

摘要： 利用农杆菌介导的高效遗传转化技术，成功地将Bar基因转入两系杂交稻的不育系培矮64S。转基因植株具有明显的Basta抗性，分子检测进一步证明Bar基因已整合到水稻染色体上。模拟制种的成功，表明机械化制备杂交水稻种子是可行的。讨论了实现机械化制备杂交水稻种子所需解决的各种问题。

关键词: 杂交水稻, 转基因, 机械化

Abstract: Using a high efficient rice transformation system mediated by Agrobacterium tumefaciens, Bar gene was introduced into a photoperiod temperature sensitive genetic male sterile line Pei'ai 64S. The transgenic plants showed the resistance to herbicide Basta. PCR and Southern blot analysis indicate that Bar gene was inserted in genomic DNA of Pei'ai 64S. Simulation on seed production of hybrid rice using transgenic Pei'ai 64S as a female parent was also in successful. This result proved the feasibility of mechanization to produce hybrid rice seeds via gene transformation. The problems on practical utilization of this protocol are discussed.

Key words: hybrid rice, gene transfer, mechanization, hybrid seed production