皖鄂地区水稻纹枯病菌致病力分化研究

doi:10.3969/j.issn.1001-7216.2010.06.010

摘要/Abstract

摘要： 采用温室苗期接种鉴定法，以抗感反应不同的5个水稻品种为材料，对从安徽省和湖北省采集的水稻纹枯病感病标样上分离的200个菌株进行了致病性鉴定。结果表明，水稻纹枯病菌不同菌株间致病力存在明显差异，在鉴别品种上的病情指数呈正态分布。基于5个鉴别品种的平均病情指数的动态聚类分析，将200个菌株划分为弱、中、强3种致病型，分别占供试菌株的29.5％、60.5％和100%，其中中等致病型菌株占优势。各致病型在地区间呈随机分布，表明自然条件下水稻纹枯病菌为混合致病群。Mantel测验表明，菌株致病力差异与菌株地域来源无明显相关。采用Bayes法建立了各致病型的判别函数，判别准确率达95.00％，说明采用聚类判别分析能够对水稻纹枯病菌致病力的分化进行合理的判断。

关键词: 水稻纹枯病菌, 致病力分化, 聚类分析, 贝叶斯判别

Abstract: Pathogenicity variation of 200 isolates of rice sheath blight (SB) pathogen Rhizoctonia solani AG1 ⅠA from the infected samples from provinces of Anhui and Hubei, China were analyzed by using five cultivars with different resistance to SB at the seedling stage in greenhouse. There were significant differences in pathogenicity among these tested isolates of R. solani AG1 ⅠA. The frequency of average disease index followed a normal school distribution. Based on their disease indices to the five cultivars, the isolates were classified into three distinct pathotype groups by dynamic cluster analysis, i.e. weak, moderate and strong pathotypes, accounting for 29.5%, 60.5% and 10.0%, respectively. The moderate pathotypes were dominant. Random distribution of pathotypes in different areas implied that R. solani AG1 ⅠA population was a mixture of pathotypes naturally. The Mantel testing revealed that the pathogenicity variation was not obviously correlated with the collective location. The discriminant functions of the three pathotypes were constructed based on the Bayes method. The accurate rate for discrimination was up to 95.00%. The results indicate that dynamic cluster and discriminant analysis can be used to evaluate the pathogenicity differentiation of R. solani AG1 ⅠA.

Key words: Rhizoctonia solani, pathogenicity differentiation, cluster analysis, Bayes discrimination

王玲,黄雯雯,黄世文,刘连盟,刘恩勇, . 皖鄂地区水稻纹枯病菌致病力分化研究[J]. 中国水稻科学 2010,24(6): 623-629 . , DOI: 10.3969/j.issn.1001-7216.2010.06.010 .

WANG Ling,HUANG Wen-wen,HUANG Shi-wen,LIU Lian-meng,LIU En-yong,. Pathogenicity Differentiation of Rice Sheath Blight Pathogen Rhizoctonia solani AG-1 ⅠA Isolates from Anhui and Hubei Provinces, China[J]. Chinese Journal of Rice Science 2010,24(6): 623-629 ., DOI: 10.3969/j.issn.1001-7216.2010.06.010 .

参考文献

[1]Guleria S, Aggarwal R, Thind T S, et al. Morphological and pathological variability in rice isolates of Rhizoctonia solani and molecular analysis of their genetic variability. J Phytopathol, 2007, 155: 654-661.

[2]张楷正, 李平, 李娜, 等. 水稻抗纹枯病种质资源、抗性遗传和育种研究进展. 分子植物育种, 2006, 4(5): 713-720.

[3]Park D S, Sayler R J, Hong Y G, et al. A method for inoculation and evaluation of rice sheath blight disease. Plant Dis, 2008, 92(1): 25-29.

[4]Singh A, Rohilla R, Singh U S, et al. An improved inoculation technique for sheath blight of rice caused by Rhizoctonia solani. Can J Plant Pathol, 2002, 24: 65-68.

[5]Zou J H, Pan X B, Chen Z X, et al. Mapping quantitative trait loci controlling sheath blight resistance in two rice cultivars (Oryza sativa L.). Theor Appl Genet, 2000, 101: 569-573.

[6]Eizenga G C, Lee F N, Rutger J N. Screening Oryza species plants for rice sheath blight resistance. Plant Dis, 2002, 86: 808-812.

[7]潘学彪, 陈宗祥, 徐敬友, 等. 不同接种调查方法对抗水稻纹枯病遗传研究的影响. 江苏农学院学报, 1997, 18(3): 27-32.

[8]Jia Y, Correa V F, McClung A, et al. Rapid determination of rice cultivar responses to the sheath blight pathogen Rhizoctonia solani using a micro-chamber screening method. Plant Dis, 2007, 91(5): 485-489.

[9]王子斌, 左示敏, 李刚, 等. 水稻抗纹枯病苗期快速鉴定技术研究. 植物病理学报, 2009, 39(2): 174-182.

[10]陈夕军, 王玲, 左示敏, 等. 水稻纹枯病寄主-病原物互作鉴别品种与菌株的筛选. 植物病理学报, 2009, 39(5): 514-520.

[11]Pascual C B, Toda T, Raymondo A D, et al. Characterization by conventional techniques and PCR of Rhizoctonia solani isolates causing banded leaf sheath blight in maize. Plant Pathol, 2000, 49: 108-118.

[12]陈延熙, 张敦华, 段霞渝, 等. 关于Rhizoctonia solani菌丝融合分类和有性世代的研究. 植物病理学报, 1985, 15(3): 139-143.

[13]许志刚, 孙启明, 刘凤权, 等. 水稻白叶枯病菌小种分化的监测. 中国水稻科学, 2004, 18(5): 469-472.

[14]Neeraja C N, Shenoy V V, Reddy C S, et al. Isozyme polymorphism and virulence of Indian isolates of the rice sheath blight fungus. Mycopathologia, 2002, 156: 101-108.

[15]Singh V, Singh U S, Singh K P, et al. Genetic diversity of Rhizoctonia solani isolates from rice: Differentiation by morphological characteristics, pathogenicity, anastomosis behavior and RAPD fingerprinting. J Mycol Plant Pathol, 2002, 32: 332-344.

[16]Liu C, Lakshman D K, Tavantzis S M. Expression of a hypovirulence-causing double-stranded RNA (dsRNA) is associated with up-regulation of quinic acid pathway, and down-regulation of shikimic acid pathway in Rhizoctonia solani. Curr Genet, 2003, 42: 284-291.

[17]Linde C C, Zala M, Paulraj R S D, et al. Population structure of the rice sheath blight pathogen Rhizoctonia solani AG-1 ⅠA from India. Eur J Plant Pathol, 2005, 112: 113-121.

[18]Dorrance A E, Kleinhenz M D, McClure S A, et al. Temperature, moisture, and seed treatment effects on Rhizoctonia solani root rot of soybean. Plant Dis, 2003, 87(5): 533-538.

[19]Taheri P, Gnanamanickam S, Hfte M. Characterization, genetic structure, and pathogenicity of Rhizoctonia spp. associated with rice sheath diseases in India. Phytopathology, 2007, 97(3): 373-383.

[20]Mahmoud Y A, Gaafar R M, Mubarak H M. Genetic diversity among Nile Delta isolates of Rhizoctonia solani Kühn based on pathogenicity, compatibility, isozyme analysis and total protein pattern. Turk J Bot, 2007, 31: 19-29.

[21]Khodayari M, Safaie N, Shamsbakhsh M. Genetic diversity of Iranian AG1-ⅠA isolates of Rhizoctonia solani, the cause of rice sheath blight, using morphological and molecular markers. J Phytopathol, 2009, 157: 708-714.

[22]智海剑, 盖钧镒, 何小红. 大豆对SMV数量（程度）抗性的综合分级方法研究. 大豆科学, 2005, 24(1): 5-11.

[23]刘波, 蓝江林, 车建美, 等. 青枯雷尔氏菌脂肪酸型与致病性的关系. 中国农业科学, 2009, 42(2): 511-522.