中国水稻科学 ›› 2018, Vol. 32 ›› Issue (6): 549-556.DOI: 10.16819/j.1001-7216.2018.8015
冯冰, 孙雅菲, 艾昊, 刘秀丽, 杨晶, 刘璐, 高飞燕, 徐国华, 孙淑斌*()
收稿日期:
2018-02-05
修回日期:
2018-06-11
出版日期:
2018-11-27
发布日期:
2018-11-10
通讯作者:
孙淑斌
基金资助:
Bing FENG, Yafei SUN, Hao AI, Xiuli LIU, Jing YANG, Lu LIU, Feiyan GAO, Guohua XU, Shubin SUN*()
Received:
2018-02-05
Revised:
2018-06-11
Online:
2018-11-27
Published:
2018-11-10
Contact:
Shubin SUN
摘要:
目的 磷对植物细胞内蔗糖和淀粉合成有重要影响,它们之间的关系还有许多方面有待阐明。方法 本研究利用转基因技术获得OsSUT1超表达材料,通过水培和盆栽实验研究了超表达该基因对蔗糖、磷含量以及植株形态和生理性状的影响。结果 转录水平上的表达分析显示,缺磷诱导OsSUT1在水稻根系中表达。水培实验显示,与野生型相比,正常供磷条件下OsSUT1超表达导致水稻植株地上部蔗糖含量下降,同时水稻植株内的磷含量升高;而在缺磷条件下植株体内蔗糖及磷含量均无显著变化。盆栽实验显示,超表达OsSUT1提高了水稻的分蘖数、有效分蘖数、磷含量、粒长和粒宽。结论 这些结果说明OsSUT1对水稻的蔗糖和磷含量以及种子的生长发育有重要作用。
中图分类号:
冯冰, 孙雅菲, 艾昊, 刘秀丽, 杨晶, 刘璐, 高飞燕, 徐国华, 孙淑斌. 超表达蔗糖转运蛋白基因OsSUT1对水稻形态和生理的影响[J]. 中国水稻科学, 2018, 32(6): 549-556.
Bing FENG, Yafei SUN, Hao AI, Xiuli LIU, Jing YANG, Lu LIU, Feiyan GAO, Guohua XU, Shubin SUN. Overexpression of Sucrose Transporter OsSUT1 Affects Rice Morphology and Physiology[J]. Chinese Journal OF Rice Science, 2018, 32(6): 549-556.
图1 OsSUT1基因在正常供磷和缺磷条件下的相对表达量^图中所显示数据为平均值±标准差(n=3)。柱上标不同字母表示差异达0.05显著水平。
Fig. 1. Relative expression level of OsSUT1 under Pi-sufficient and Pi-deficient conditions.^ Values are mean±SD(n=3) and different letters indicate that the values differ significantly (P < 0.05).
图2 qRT-PCR鉴定OsSUT1-Ox植株超表达效果^图中所显示数据为平均值±标准差(n=3)。柱上标不同字母表示差异未达0.05显著水平。WT–野生型。
Fig. 2. qRT-PCR analysis of the expression levels of OsSUT1 in OsSUT1-Ox plants.^ Values are means±SD(n=3) and different letters indicate that the values differ significantly (P < 0.05). WT, Wild type.
图3 不同供磷条件下野生型和超表达材料蔗糖含量^图中数据为平均值±标准差(n=5)柱上不同小写字母表示差异达0.05显著水平。
Fig. 3. Sucrose concentration of WT and over-expressed plants under Pi-sufficient and Pi-deficient conditions.^ Values are mean±SD (n = 5) and different lowercase letters indicate that the values differ significantly (P < 0.05).
图4 不同供磷条件下野生型与超表达材料表型分析^ A和D,正常供磷(A)和缺磷(D)条件下野生型和OsSUT1超表达材料的苗期表型,标尺为5 cm;图中数据为平均值±标准差,n = 5。柱上标相同小写字母者表示差异未达0.05显著水平。
Fig. 4. Phenotype analysis of wild type(WT) and over-expressed plants under Pi-sufficient and Pi-deficient conditions.^ A and D, Phenotype under Pi-sufficient (A) and Pi-deficient (D) conditions, Bar = 5 cm; Values are mean±SD (n = 5) and different letters indicate that the values differ significantly (P < 0.05).
图5 不同供磷条件下野生型和超表达材料有效磷含量^ A,正常供磷条件下野生型和OsSUT1超表达材料有效磷含量;B,缺磷条件下野生型和OsSUT1超表达材料有效磷含量;图中显示数据为平均值±标准差,n = 5。柱上标相同小写字母者表示差异未达0.05显著水平。
Fig. 5. Pi concentration of wild type(WT) and over-expressed plants under Pi-sufficient and Pi-deficient conditions.^ A, Pi concentration of WT and OsSUT1-Ox materials under Pi-sufficient conditions; B, Pi concentration of WT and OsSUT1-Ox materials under Pi-deficient conditions. Values are mean±SD (n = 5) and different letters indicate that the values differ significantly (P < 0.05).
图6 正常供磷条件下盆栽实验中野生型和超表达材料各部位总磷含量^图中显示数据为平均值±标准差,n = 5。柱上标相同小写字母者表示差异未达0.05显著水平。
Fig. 6. Total P concentration in different tissues of WT and over-expressed plants in Pi-sufficient pot experiments.^ Values are mean±SD (n = 5) and different letters on the histograms indicate that the values differ significantly (P< 0.05).
图7 正常供磷条件下盆栽实验中野生型和超表达材料表型^ A–野生型和OsSUT1突变体材料成熟期盆栽表型,标尺为10 cm;B–成熟期野生型和OsSUT1突变体材料的植株高度;C–成熟期野生型和OsSUT1突变体材料的分蘖数;D,成熟期野生型和OsSUT1突变体材料的有效分蘖数。平均值±标准差,n=10, 柱上标相同字母者表示差异未达0.05显著水平。
Fig. 7. Phenotype of wild type(WT) and over-expressed plants in Pi-sufficient pot experiments.^ A, WT and OsSUT1 mutant in mature stage, Bar = 10cm; B, Plant height of WT and OsSUT1 mutant; C, Tiller number of WT and OsSUT1 mutant; D, Effective tiller number of WT and OsSUT1 mutant. Values are mean±SD (n = 10) and different letters indicate that the values differ significantly (P < 0.05).
图8 野生型与OsSUT1超表达材料种子形态和统计数据^ A和B–野生型和OsSUT1超表达材料籽粒表型;标尺为1 cm。 C–粒长;D–粒宽。生物学重复为20个;图中显示数据为平均值±标准差,n = 20。柱上不同小写字母者表示差异未达0.05显著水平。。
Fig. 8. Seed morphology and statistics of WT and OsSUT1-Ox materials.^ A and B, Grain phenotype of WT and OsSUT1-Ox materials; Bar = 1 cm. C, Grain length; D, Grain width. Values are mean±SD (n=20) and different letters indicate that the values differ significantly (P < 0.05).
[1] | 郭志奇. 我国水稻栽培现状、高产栽培技术及展望. 南方农业, 2014(8): 92-93. |
Guo Z Q.Current status of rice cultivation, high yield cultivation techniques and prospects in China.South China Agric, 2014(8): 92-93. | |
[2] | 张亮, 孙文献, 孙雅菲, 裴文霞, 罗闻真, 孙瑞, 张占田, 徐国华, 孙淑斌. 水稻转录因子基因OsPHR3在磷素利用过程中的作用. 中国水稻科学, 2016, 30(4): 397-405. |
Zhang L, Sun W X, Sun Y F, Pei W X, Luo W Z, Sun R, Zhang Z T, Xu G H, Sun S B.Roles of transcription factor gene OsPHR3 on the utilization of phosphate in rice. Chin J Rice Sci, 2016, 30(4): 397-405. (in Chinese with English abstract) | |
[3] | Patrick J W, Botha F C, Birch R G.Metabolic engineering of sugars and simple sugar derivatives inplants.Plant Biotechnol J, 2013, 11: 142-156. |
[4] | 李合生. 植物生理生化实验原理和技术. 北京: 高等教育出版社, 2001. |
Li H S.Physiological and biochemical experimental principles and techniques. Beijing: Higher Education Press, 2001. (in Chinese) | |
[5] | Rae A L, Perroux J M, Grof C P.Sucrose partitioning between vascular bundles and storage parenchyma in the sugarcane stem: A potential role for the ShSUT1 sucrose transporter.Planta, 2005, 220: 817-825. |
[6] | Chen L Q, Qu X Q, Hou B H, Sosso D, Osorio S, Fernie A R, Frommer W B.Sucrose efflux mediated by SWEET proteins as a key step for phloem transport.Science, 2012, 335: 207-211. |
[7] | Chen L Q, Lin I W, Qu X Q, Sosso D, McFarlane H E, Londoño A, Samuels A L, Frommer W B. A cascade of sequentially expressed sucrose transporters in the seed coat and endosperm provides nutrition for theArabidopsis embryo. Plant Cell, 2015, 27: 607-619. |
[8] | Ma L, Zhang D, Miao Q, Yang J, Xuan Y, Hu Y.Essential role of sugar transporter OsSWEET11 during the early stage of rice grain filling.Plant Cell Physiol, 2017, 58: 863-873. |
[9] | Heldt H W, Flugge U I.Metabolite transport in plant cells.Sem Ser: Soc Exper Biol, 1992(50): 21-47. |
[10] | Lalonde S, Wipf D, Frommer W B.Transport mechanisms for organic forms of carbon and nitrogen between source and sink.Annu Rev Plant Biol, 2004, 55: 341-372. |
[11] | Williams L E, Lemoine R, Sauer N.Sugar transporters in higher plants-A diversity of roles and complex regulation.Trends Plant Sci, 2000, 5: 283-290. |
[12] | Scofield G N, Hirose T, Aoki N, Furbank R T.Involvement of the sucrose transporter, OsSUT1, in the long-distance pathway for assimilate transport in rice. J Eex Bot, 2007, 58: 3155-3169. |
[13] | Wang L, Lu Q, Wen X, Lu C.Enhanced sucrose loading improves rice yield by increasing grain size.Plant Physiol, 2015, 169: 2848-2862. |
[14] | Matsukura C, Saitoh T, Hirose T, Ohsugi R, Perata P, Yamaguchi J.Sugar uptake and transport in rice embryo: Expression of companion cell-specific sucrose transporter (OsSUT1) induced by sugar and light.Plant Physiol, 2000, 124: 85-93. |
[15] | Cai Y, Tu W, Zu Y, Yan J, Xu Z, Lu J, Zhang Y.Overexpression of a grapevine sucrose transporter (VvSUC27) in tobacco improves plant growth rate in the presence of sucrosein vitro.Front Plant Sci, 2017, 8: 1069. |
[16] | Chen J Y, Liu S L, Wei S, Wang S J.Hormone and sugar effects on rice sucrose transporter OsSUT1, expression in germinating embryos.Acta Physiol Plant, 2010, 32: 749-756. |
[17] | Zakhleniuk O V, Raines C A,Lloyd J C. pho3: A phosphorus-deficient mutant of Arabidopsis thaliana. Planta, 2001, 212: 529-534. |
[18] | Lloyd J C, Zakhleniuk O V.Responses of primary and secondary metabolism to sugar accumulation revealed by microarray expression analysis of theArabidopsis mutant, pho3. J Exp Bot, 2004, 55: 1221-1230. |
[19] | Wang L, Lu Q, Wen X, Lu C.Enhanced sucrose loading improves rice yield by increasing grain size.Plant Physiol, 2015, 169: 2848-2862. |
[20] | Jansson C.Mutation: sugar signaling mutants inArabidopsis. Prog Bot, 2005, 66: 50-67. |
[21] | Chiou T J, Bush D R.Sucrose is a signal molecule in assimilate partitioning.Proc Natl Acad Sci USA, 1998, 95: 4784-4788. |
[22] | Eom J S, Choi S B, Ward J M, Jeon J S.The mechanism of phloem loading in rice (Oryza sativa). Mol Cells, 2012, 33: 431-438. |
[23] | Lei M, Liu Y, Zhang B, Zhao Y, Wang X, Zhou Y, Raghothama K G, Liu D.Genetic and genomic evidence that sucrose is a global regulator of plant responses to phosphate starvation inArabidopsis. Plant Physiol, 2011, 156: 1116-1130. |
[24] | Scofield G N, Aoki N, Hirose T, Takano M, Jenkins C L, Furbank R T.The role of the sucrose transporter, OsSUT1, in germination and early seedling growth and development of rice plants.J Exp Bot, 2007, 58: 483-495. |
[25] | Ishimaru T, Ida M, Hirose S, Shimamura S, Masumura T, Nishizawa N K, Nakazono M, Kondo M.Laser microdissection-based gene expression analysis in the aleurone layer and starchy endosperm of developing rice caryopses in the early storage phase.Rice, 2015, 8: 0015-0057. |
[26] | Tognetti J A, Pontis H G, Martínez-Noël G M A. Sucrose signaling in plants: A world yet to be explored. Plant Signal Behav, 2013, 8: 3, e23316. |
[1] | 任志奇, 薛可欣, 董铮, 李小湘, 黎用朝, 郭玉静, 刘文强, 郭梁, 盛新年, 刘之熙, 潘孝武. 水稻外卷叶突变体ocl1的鉴定及基因定位[J]. 中国水稻科学, 2023, 37(4): 337-346. |
[2] | 肖乐铨, 李雷, 戴伟民, 强胜, 宋小玲. 转cry2A*/bar基因水稻与杂草稻杂交后代的苗期生长特性[J]. 中国水稻科学, 2023, 37(4): 347-358. |
[3] | 李刚, 高清松, 李伟, 张雯霞, 王健, 程保山, 王迪, 高浩, 徐卫军, 陈红旗, 纪剑辉. 定向敲除SD1基因提高水稻的抗倒性和稻瘟病抗性[J]. 中国水稻科学, 2023, 37(4): 359-367. |
[4] | 汪胜勇, 陈宇航, 陈会丽, 黄钰杰, 张啸天, 丁双成, 王宏伟. 水稻减数分裂期高温对苯丙烷类代谢及下游分支代谢途径的影响[J]. 中国水稻科学, 2023, 37(4): 368-378. |
[5] | 董立强, 杨铁鑫, 李睿, 商文奇, 马亮, 李跃东, 隋国民. 株行距配置对超高产田水稻产量及根系形态生理特性的影响[J]. 中国水稻科学, 2023, 37(4): 392-404. |
[6] | 韩聪, 何禹畅, 吴丽娟, 郏丽丽, 王磊, 鄂志国. 水稻碱性亮氨酸拉链(bZIP)蛋白家族功能研究进展[J]. 中国水稻科学, 2023, 37(4): 436-448. |
[7] | 沈雨民, 陈明亮, 熊焕金, 熊文涛, 吴小燕, 肖叶青. 水稻内外稃异常发育突变体blg1 (beak like grain 1)的表型分析与精细定位[J]. 中国水稻科学, 2023, 37(3): 225-232. |
[8] | 段敏, 谢留杰, 高秀莹, 唐海娟, 黄善军, 潘晓飚. 利用CRISPR/Cas9技术创制广亲和水稻温敏雄性不育系[J]. 中国水稻科学, 2023, 37(3): 233-243. |
[9] | 程玲, 黄福钢, 邱一埔, 王心怡, 舒宛, 邱永福, 李发活. 籼稻材料570011抗褐飞虱基因的遗传分析及鉴定[J]. 中国水稻科学, 2023, 37(3): 244-252. |
[10] | 王文婷, 马佳颖, 李光彦, 符卫蒙, 李沪波, 林洁, 陈婷婷, 奉保华, 陶龙兴, 符冠富, 秦叶波. 高温下不同施肥量对水稻产量品质形成的影响及其与能量代谢的关系分析[J]. 中国水稻科学, 2023, 37(3): 253-264. |
[11] | 刘嫒桦, 李小坤. 不同肥料施用与稻米品质关系的整合分析[J]. 中国水稻科学, 2023, 37(3): 276-284. |
[12] | 杨晓龙, 王彪, 汪本福, 张枝盛, 张作林, 杨蓝天, 程建平, 李阳. 不同水分管理方式对旱直播水稻产量和稻米品质的影响[J]. 中国水稻科学, 2023, 37(3): 285-294. |
[13] | 魏晓东, 宋雪梅, 赵凌, 赵庆勇, 陈涛, 路凯, 朱镇, 黄胜东, 王才林, 张亚东. 硅锌肥及其施用方式对南粳46产量和稻米品质的影响[J]. 中国水稻科学, 2023, 37(3): 295-306. |
[14] | 林聃, 江敏, 苗波, 郭萌, 石春林. 水稻高温热害模型研究及其在福建省的应用[J]. 中国水稻科学, 2023, 37(3): 307-320. |
[15] | 郑承梅, 孙金秋, 刘梦杰, 杨永杰, 陆永良, 郭怡卿, 唐伟. 水稻田糠稷种子萌发和出苗特性及化学防除药剂筛选[J]. 中国水稻科学, 2023, 37(3): 321-328. |
阅读次数 | ||||||
全文 |
|
|||||
摘要 |
|
|||||