中国水稻科学 ›› 2018, Vol. 32 ›› Issue (5): 427-436.DOI: 10.16819/j.1001-7216.2018.7037

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水稻转录因子基因OsSHR2的表达特征及其在营养生长中的调控作用

张占田, 孙雅菲, 艾昊, 罗闻真, 冯冰, 孙文献, 徐国华, 孙淑斌*()   

  1. 南京农业大学 资源与环境科学学院, 南京 210095;
  • 收稿日期:2017-03-30 修回日期:2017-08-18 出版日期:2018-09-10 发布日期:2018-09-10
  • 通讯作者: 孙淑斌
  • 基金资助:
    国家自然科学基金资助项目(31672226);国家转基因植物研究计划资助项目(2016ZX08009-003-005);江苏省自然科学基金资助项目(BK20141367)

Expression Patterns and Regulation of Transcription Factor Gene OsSHR2 in Vegetative Growth in Rice

Zhantian ZHANG, Yafei SUN, Hao AI, Wenzhen LUO, Bing FENG, Wenxian SUN, Guohua XU, Shubin SUN*()   

  1. College of Resources and Environmental Science, Nanjing Agricultural University, Nanjing 210095, China;
  • Received:2017-03-30 Revised:2017-08-18 Online:2018-09-10 Published:2018-09-10
  • Contact: Shubin SUN

摘要:

【目的】水稻OsSHR2(LOC_Os03g31880)基因为拟南芥AtSHR的同源基因,与OsSHR1OsSCR1OsSCR2 同属于水稻GRAS转录因子家族。已有研究报道,转录因子基因SHRSCR共同调控植物根系、叶片的发育,并参与各项生命活动。本研究旨在阐明OsSHR2在水稻中的时空表达特征及其在营养生长中的调控作用。【方法】通过生物信息学分析、表达模式分析、萌发动力学分析和水培实验验证该基因的功能。【结果】生物信息学分析发现OsSHR2OsSHR1OsSCR1OsSCR2与拟南芥和其他物种的SHR亚家族和SCR亚家族成员具有很高的序列一致性;表达模式和pOsSHR2::GUS材料染色分析发现,OsSHR2在整个生长发育过程中的根系、叶片、维管组织和生殖器官中表达强烈,并集中在根尖的中柱、侧根原基和叶片及茎维管组织的中心表达,在野生型的地上部和根系中,OsSHR2受缺磷影响下调表达;对获得的OsSHR2的CRISPR-Cas9突变体osshr2进行种子萌发实验和水培实验,发现与野生型相比,osshr2的萌发时间延后,萌发率降低,在正常供磷和缺磷处理下,osshr2的地上部和根系长度显著小于野生型。【结论】OsSHR2在地上部和根系的发育、维管组织形成以及营养与生殖生长中具有重要作用,这为今后OsSHR2在分子育种等领域的应用奠定理论基础。

关键词: 水稻, OsSHR2, 时空表达特征, 营养生长,

Abstract:

【Objective】OsSHR2(LOC_Os03g31880) is a homologous gene of AtSHR of Arabidopsis thaliana, which falls into GRAS transcription factor family together with OsSHR1, OsSCR1 and OsSCR2 in rice. It has been reported that the transcription factor genes SHR and SCR regulate the development of roots and leaves, and participate in various life activities. We analyzed the temporal and spatial expression patterns and the way OsSHR2 regulates vegetative growth in rice.【Method】The function of OsSHR2 was verified by bioinformatics analysis, expression pattern analysis, germination kinetic analysis and hydroponic experiments.【Result】 Biomechanical analysis showed that OsSHR2, OsSHR1, OsSCR1 and OsSCR2 had high homology with SHR subfamily and SCR subfamily in Arabidopsis thaliana and other species. The expression pattern analysis by qRT-PCR and pOsSHR2::GUS staining showed that OsSHR2 was strongly expressed in the roots, leaves, vascular tissues and reproductive organs during the whole vegetative and reproductive growth stages, and especially in the stele of the root tip, lateral root primordium and the central of leaf and stem vascular tissue. Additionally, the relative expression of OsSHR2 was down-regulated in Pi-deficiency in shoots and roots of wild type. The seed germination and hydroponic experiment analysis of CRISPR-Cas9 mutant osshr2 showed that seed germination of osshr2 was delayed and with lower germination rate than that of WT, in addition the length of shoot and root of osshr2 were significantly shorter than WT under Pi-sufficient and Pi-deficient conditions.【Conclusion】OsSHR2 plays an important role in the development of shoots and roots, the formation of vascular tissue and various activity in vegetative and reproductive growth, which lays an important theoretical basis for the application of OsSHR2 in molecular breeding.

Key words: rice, OsSHR2, temporal and spatial expression pattern, vegetative growth, phosphorus

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