中国水稻科学 ›› 2017, Vol. 31 ›› Issue (1): 40-49.DOI: 10.16819/j.1001-7216.2017.6117

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长江流域主要常规双季早籼稻的遗传相似性分析及指纹图谱构建

戴冬青, 张华丽, 张萌, 李西明, 马良勇*()   

  1. 中国水稻研究所, 杭州 310006
  • 收稿日期:2016-08-01 修回日期:2016-09-24 出版日期:2017-01-20 发布日期:2017-01-10
  • 通讯作者: 马良勇
  • 基金资助:
    国家863计划资助项目(2014AA10A604-15);国家自然科学基金资助项目(31501288);浙江省自然科学基金资助项目(LQ16C130002);浙江省高产优质多抗水稻新品种选育(9410计划)资助项目(2012C12901-2);中国水稻研究所中央级公益性科研院所基本科研业务费专项(2014RG003-3)

Analysis of Genetic Similarity and Construction of DNA Fingerprinting for Conventional Double Cropping Early Rice Varieties in the Yangtze River Basin

Dongqing DAI, Huali ZHANG, Meng ZHANG, Ximing LI, Liangyong MA*()   

  1. China National Rice Research Institute, Hangzhou 310006, China
  • Received:2016-08-01 Revised:2016-09-24 Online:2017-01-20 Published:2017-01-10
  • Contact: Liangyong MA

摘要:

目的 构建常规早稻品种的指纹图谱以及分析其遗传相似性,为早稻品种鉴定、育种者有效选配亲本提供参考依据。方法 以2000年以来长江流域双季稻区通过省级以上审定的常规早稻品种为材料,利用50对多态性高,稳定性好,且均匀分布于水稻12条染色体的SSR引物,建立常规早稻的SSR指纹图谱。利用NTSYS PC Version 2.10e 软件、UPGMA法进行聚类分析。结果 50对引物共检测到多态性位点154个,每对引物检测到的等位基因数为2~6个,平均3.08个;引物多态信息含量(PIC)值为0.03~0.75,平均值为0.36。62份材料的遗传相似系数变化范围为0.66~1,并且在相似系数为0.73处可分为浙江省内品种及省外品种2组,各组间差异不大。结论 结合系谱关系发现,本研究中所用早稻品种的遗传背景较为狭窄。

关键词: 早稻, 系谱, DNA指纹图谱, 聚类分析

Abstract:

【Objective】 To provide a reference for rice variety identification and parents selection,we constructed fingerprints of conventional early rice varieties and analyzed its genetic similarity. 【Method】 Conventional early rice varieties registered at or above the provincial level since 2000 in the Yangtze River Basin were analyzed using 50 pairs of SSR primers with high polymorphism, good stability, and even distribution on 12 chromosomes of rice to construct SSR fingerprints of conventional early rice. Cluster analysis was conducted with NTSYS PC Version 2.10e software and UPGMA cluster analysis method. 【Results】 A total of 154 polymorphic bands were detected among 62 rice lines. Two to six alleles were detected for each marker with an average of 3.08. Primers polymorphism information content (PIC) value ranged from 0.03 to 0.75, with an average of 0.36. Cluster analysis with NTSYS PC Version 2.10e software showed that the genetic similarities ranged from 0.66 to 1.00. The 62 varieties could be divided into two groups as varieties in Zhejiang Province and varieties outside the province at the similarity coefficient of 0.73 with small difference between groups. 【Conclusion】 Combination with the pedigree relationship, it was revealed that the genetic background of early rice varieties in this study was relatively narrow.

Key words: early rice, pedigree, DNA fingerprinting, clustering analysis

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