Chinese Journal OF Rice Science

Chinese Journal OF Rice Science >

2009 , Vol. 23 >Issue 4: 349 - 353

DOI: https://doi.org/10.3969/j.issn.1001-7216.2009.04.03

研究报告

Effect of Rhizosphere pH on the Expression of Plasma Membrane H+ATPase Gene in Rice Plants

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  • College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China; *Corresponding author, E-mail: ghxu@njau.edu.cn

Received date: 1900-01-01

  Revised date: 1900-01-01

  Online published: 2009-07-10

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Abstract

Effect of rhizosphere pH on the expression of plasma membrane H+ATPase genes (PMA) in rice (Oryza sativa ssp. japonica cv. Nipponbare) was investigated. Rice seedlings were grown in a solution culture at pH 5.5 as control, and then transferred to the solution at pH 3.5 and 7.5, respectively. Expressions of OsPMA1, OsPMA2, OsPMA3, OsPMA7 were detected in leaves and roots. The expression levels of the PMA genes were lower at pH 3.5 or pH 7.5 than that at pH 5.5. The biomass and N content in rice seedlings decreased with the decreasing pH. The results indicated that the expression of plasma membrane H+ATPase genes in rice roots were inhibited at pH 3.5 by suddenly increased H+ concentration outside plant cells. In this way, the uptake of nutrients driven by plasma membrane H+ATPase was disturbed. Because pH 7.5 in root medium is equivalent to the cytosolic pH value, plasma membrane H+ATPase is not necessary to be overexpressed to overcome the higher H+ gradient across the plasma membrane as at low pH medium (pH 5.5 or pH 3.5). The rest of H+ATPase might be enough for the coupling of nutrient uptake. It is suggested that the rhizosphere pH had great influence on the transcriptional expression of plasma membrane H+ATPase and interfered the nutrient uptake and plant growth.

Key words： rice; plasma membrane; H+ATPase; hydrogen ion concentration(pH); gene expression; rhizophere

Cite this article

SHEN Jing,ZHU Yiyong,XU Guohua* . Effect of Rhizosphere pH on the Expression of Plasma Membrane H+ATPase Gene in Rice Plants [J]. Chinese Journal OF Rice Science, 2009 , 23(4) : 349 -353 . DOI: 10.3969/j.issn.1001-7216.2009.04.03

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