Chinese Journal OF Rice Science >
Development and Application of Functional Markers for Purity Identification of Wild-abortive Three-line Hybrid Rice
Received date: 2025-02-14
Revised date: 2025-07-15
Online published: 2026-03-16
【Objective】 Developing stable, efficient, and reliable markers for purity testing of three-line hybrid seeds is crucial for current hybrid rice seed production. 【Method】 Based on the specific sequences of the wild abortive-type sterility gene WA352, the major fertility-restoring gene Rf4, and the internal reference gene Actin, multiple single-gene detection markers were designed. PCR amplification was then performed by optimizing the primer mixture ratio. 【Result】 A mixture of primers WA352-2, Rf4-3, and Actin-3 in a ratio of 1:11:3 enabled simultaneous detection of the genotypes of WA352, Rf4, and Actin in a single PCR reaction, allowing differentiation among wild abortive-type CMS lines, restorer lines, maintainer lines, and hybrids. 【Conclusion】 The detection method established in this study is simple to perform and yields stable and reliable results. It can not only identify genotypes in wild-abortive three-line rice but also serve as an effective tool for molecular marker-assisted breeding. By improving the accuracy of purity assessment during breeding, this method provides strong support for achieving high-yield and high-quality rice breeding objectives in China.
Key words： rice; wild-abortive; three-line; detection marker; PCR
ZHANG Mengke, LU Jiayu, HE Jin, XU Xue, WU Shuang, WANG Peiran, CHEN Ruofan, JIN Qing, WANG Xiufeng . Development and Application of Functional Markers for Purity Identification of Wild-abortive Three-line Hybrid Rice[J]. Chinese Journal OF Rice Science, 2026 , 40(2) : 244 -252 . DOI: 10.16819/j.1001-7216.2026.250204
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