Chinese Journal OF Rice Science >
Modifying Heading Date of Nanjing 46 via CRISPR/Cas9-mediated Genome Editing
Received date: 2024-07-09
Revised date: 2024-08-12
Online published: 2025-11-19
【Objective】 Heading date is a key agronomic trait determining the regional and ecological adaptation of rice cultivars. We edited the heading date genes of the medium-maturing late japonica rice variety Nanjing 46 (NJ46) with excellent tasting to shorten its heading date thereby potentially expanding its planting area.【Method】 Using CRISPR/Cas9 technology, the editing constructs, pCAMBIA1305-Actin:Cas9-sgRNADTH8 and pCAMBIA1305-Actin:Cas9-sgRNAGhd2 targeting DTH8 and Ghd2, respectively, were developed and transformed into NJ46 via Agrobacterium-mediated transformation. Homozygous editing lines without exogenous components were screened and evaluated on heading date, agronomic traits and quality-related traits. 【Result】 Three homozygous mutant lines were obtained. The NJ46-dth8 lines (NJ46-dth8-1 to -3) presented 22-36 days earlier than NJ46 on heading date, while the NJ46-ghd2 lines (NJ46-ghd2-1 to -3) showed 14-15 days earlier. The heading date, major agronomic traits, and quality related components of the homozygous mutant lines were evaluated in different areas of central and northern regions of Jiangsu Province, and compared with the widely cultivated varieties Nanjing 9108 and Nanjing 518 in their respectively ecological zones. NJ46-ghd2-1 exhibited a significantly higher yield per plant than Nanjing 9108, while showing comparable heading date and quality traits, and performed better than the other two knockout lines. NJ46-dth8-1 showed no significant differences from Nanjing 518 in yield per plant and major quality traits, and performed better than the other two knockout lines.【Conclusion】 Knockout of DTH8 and Ghd2 genes in NJ46 yielded new germplasms suitable for planting in the northern and central region of Jiangsu Province, respectively, which will help expand the planting area of this excellent-tasting rice variety.
Key words： rice; CRISPR/Cas9; genetic editing; heading date; DTH8; Ghd2
CHEN Wei, YE Yuanmei, ZHAO Jianhua, FENG Zhiming, CHEN Zongxiang, HU Keming, ZUO Shimin . Modifying Heading Date of Nanjing 46 via CRISPR/Cas9-mediated Genome Editing[J]. Chinese Journal OF Rice Science, 2025 , 39(6) : 760 -770 . DOI: 10.16819/j.1001-7216.2025.240707
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