Chinese Journal OF Rice Science >
Plant Prime Editing: A New Direction in Crop Breeding
Received date: 2024-10-16
Revised date: 2024-11-28
Online published: 2025-11-19
Plant Prime Editing (PPE) provides a novel approach for precise genome modification in plants, overcoming the limitations of conventional gene editing methods that rely on double-strand breaks and exogenous donor DNA. It enables not only arbitrary base substitutions, but also precise insertions and deletions of small to large DNA fragments, with reduced off-target effects. This review systematically summarizes the latest research advances in PPE technology globally, including its development history and working mechanism, applications in crop breeding and trait improvement, and representative cases. It also discusses the expansion of prime editing into large-scale genome editing and multiplex gene editing. To address the bottlenecks faced by PPE systems in plant genetic transformation, several potential solutions are proposed. Finally, the broad application prospects of PPE technology in plant genetic improvement and its potential integration with artificial intelligence (AI) in future research are outlined.
SONG Anqi, WU Songquan, MA Qiuyue, BAN Wanning, LIU Xiangguo, JIN Yongmei . Plant Prime Editing: A New Direction in Crop Breeding[J]. Chinese Journal OF Rice Science, 2025 , 39(6) : 711 -730 . DOI: 10.16819/j.1001-7216.2025.241005
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