Chinese Journal OF Rice Science >
Effects of Nano-molybdenum on Root Morpho-physiological Traits and Nitrate Uptake in Rice
Received date: 2024-10-17
Revised date: 2025-03-07
Online published: 2025-09-10
【Objective】 Molybdenum (Mo) serves as an essential active component of nitrate reductase, playing a pivotal role in the reduction of nitrate nitrogen and nitrogen metabolism. This study aims to investigate the relationship between nano-MoO₃ and rice root morphology, organic acid secretion, and nitrate absorption, laying a theoretical basis for improving nitrate nitrogen absorption and utilization in rice through novel nano-fertilizers. 【Method】 Using Nanjing 9108 rice as the research material, hydroponic experiments with nitrate nitrogen as the nitrogen source were conducted with treatments of no Mo application (Mo concentration, 0 μg/L), sodium molybdate (50, 100, 200, 400 μg/L), and nano-MoO₃ (50, 100, 200, 400 μg/L). Differences in rice root morphology, root-secreted organic acid, and nitrogen content were analyzed. 【Result】 Exogenous Mo significantly increased rice root dry weight, total root length, root volume, total root surface area, total absorption area, and active absorption area, significantly optimizing rice root morphology compared to the no Mo treatment. At the same Mo application rates, nano-MoO₃ treatments showed significantly higher enhancement effects than sodium molybdate treatments. Root oxidation and reduction capacities, as well as organic acid secretion, increased significantly in response to exogenous Mo treatments, indicating enhanced root physiological activity. As Mo concentrations increased, the root oxidation and reduction capacities under sodium molybdate treatments initially increased and then decreased. Except for acetic acid, there were no differences in the secretion of malic acid, tartaric acid, succinic acid, citric acid, and oxalic acid at various sodium molybdate concentrations. However, both root oxidation/reduction capacities and organic acid secretion in nano-MoO₃ treatments increased with rising application rates. Factor analysis and redundancy analysis results indicated that rice root morphological indicators, root redox capacities, and organic acid secretion were positively correlated with root nitrogen content. Exogenous Mo addition promoted nitrate nitrogen absorption by rice roots through improvements in root morphological and physiological traits. 【Conclusion】 Exogenous Mo enhanced rice root morphological and physiological activities by increasing root length, root surface area, redox capacities, and organic acid secretion, thereby promoting nitrate nitrogen absorption. Nano-MoO₃ demonstrated superior promotional effects compared to sodium molybdate at the same Mo application rates. In agricultural production, appropriate Mo fertilizer addition, especially nano-MoO₃ fertilizer, can improve rice root morphological and physiological functions, enhancing nitrate nitrogen absorption and utilization.
Key words： nano-molybdenum; rice; root morphology; root redox capacity; root exudates; nitrate nitrogen
ZHANG Haipeng, LI Wanyi, LIAO Fuxing, MA Meizi, ZHANG Hongcheng, YANG Yanju . Effects of Nano-molybdenum on Root Morpho-physiological Traits and Nitrate Uptake in Rice[J]. Chinese Journal OF Rice Science, 2025 , 39(5) : 650 -664 . DOI: 10.16819/j.1001-7216.2025.241006
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