Chinese Journal OF Rice Science >
Functional Study on Aluminum Tolerance of OsAlR1 Gene in Rice
Received date: 2024-05-19
Revised date: 2024-09-27
Online published: 2025-09-10
【Objective】 With the aggravation of soil acidification, aluminum (Al) toxicity in acidic soil seriously limits the growth and development of rice. Mining Al-tolerant gene resources, analyzing their molecular functions, and breeding Al-tolerant varieties can ensure rice yield in acidic soil. 【Method】 CRISPR/Cas9 technology was used to construct knockout vectors. The overexpression vector was constructed by homologous recombination and Golden Gate seamless cloning. Transgenic materials were obtained by genetic transformation. Homozygous overexpression lines and knockout lines were screened for Al tolerance phenotype analysis and determination of reactive oxygen species (ROS) content and antioxidant enzyme activities.【Result】 qRT-PCR results showed that OsAlR1 expression was induced by Al stress. Knockout of OsAlR1 affected root development in rice. After Al treatment, compared with the wild type, the osalr1-3 and osalr1-6 lines accumulated more Al³⁺ and ROS, while the OE-OsAlR1-5 and OE-OsAlR1-8 lines accumulated less Al³⁺ and ROS. Knockout of OsAlR1 led to decreased glutathione (GSH) content and superoxide dismutase (SOD) activity in roots. qRT-PCR results revealed that genes regulating the phenylpropanoid biosynthesis pathway (OsPAL7, OsHCT4, OsCCR18, Os02g0467600, PRX82, and Os06g0522300) showed significant expression changes in the knockout lines after Al treatment.【Conclusion】 OsAlR1 expression is induced by Al stress, and OsAlR1 alleviates ROS-mediated oxidative stress by increasing GSH content and SOD activity. OsAlR1 may affect Al tolerance in rice by regulating the phenylpropanoid biosynthesis pathway.
Key words： rice; aluminum stress; roots; ROS; antioxidant enzyme
WANG Jingbo, SU Chang, FENG Jing, JIANG Sixu, XU Hai, CUI Zhibo, ZHAO Minghui . Functional Study on Aluminum Tolerance of OsAlR1 Gene in Rice[J]. Chinese Journal OF Rice Science, 2025 , 39(5) : 615 -623 . DOI: 10.16819/j.1001-7216.2025.240510
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