Chinese Journal OF Rice Science >
Development and Application of KASP Marker for Broad-Spectrum Resistance Gene PigmR to Rice Blast
Received date: 2024-09-08
Revised date: 2025-02-25
Online published: 2025-05-21
【Objective】The broad-spectrum resistance gene PigmR has important breeding value for the genetic improvement of rice blast resistance. Developing kompetitive allele specific PCR (KASP) markers based on its coding region variation sites is beneficial for improving the accuracy and efficiency of PigmR utilization in breeding.【Method】This study identified single nucleotide variations in the coding region of PigmR through sequence alignment, developed the KASP molecular marker PigmR-K, conducted genotype analysis using rice varieties and F2 segregating populations, combined these results with rice blast symptom identification, and carried out molecular marker assisted selection breeding to verify the precision and efficiency of this marker.【Result】Based on an A-to-C mutation at position 2503 in the PigmR gene coding region sequence, a precise and efficient PigmR genotyping technology system based on KASP markers was established. The genotyping of 159 rice varieties, 270 F2 individuals, and 247 BC1F3 population lines demonstrated that PigmR-K reliably distinguished the homozygous resistant, homozygous susceptible and heterozygous genotypes of PigmR. These results were completely consistent with disease symptoms from artificial panicle blast inoculation.【Conclusion】The KASP marker PigmR-K developed based on the variation site of on the coding region has advantages such as precision and efficiency, which is beneficial for genetic improvement of rice blast resistance.
Key words： rice; broad-spectrum resistance; rice blast; PigmR gene; KASP; breeding efficiency
LI Wenqi, XU Yang, WANG Fangquan, ZHU Jianping, TAO Yajun, LI Xia, FAN Fangjun, JIANG Yanjie, CHEN Zhihui, YANG Jie . Development and Application of KASP Marker for Broad-Spectrum Resistance Gene PigmR to Rice Blast[J]. Chinese Journal OF Rice Science, 2025 , 39(3) : 365 -372 . DOI: 10.16819/j.1001-7216.2025.240104
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