Chinese Journal OF Rice Science >
Development of a New High-Quality and Multi-Resistant japonica Rice Variety, Huxianggeng 216, Through Molecular Pyramiding Breeding
#These authors contributed equally to this work
Received date: 2024-02-04
Revised date: 2024-04-26
Online published: 2025-03-19
【Objective】 Good eating quality and comprehensive resistance have always been important directions in the breeding of high-quality japonica varieties. To meet the demand for high-quality and multi-resistant rice varieties in production, it is urgent to enhance the synergistic improvement of quality and resistance and correct unfavorable traits in the breeding process. 【Method】Nangeng 46, a high-quality japonica rice variety harboring the fragrance gene badh2-E2 and the low-amylose-content gene Wxmp, was employed as the female parent, while Wuxianggeng 1, a Luzon-flavor high-quality japonica variety, served as the male parent for cross-breeding. During the self-breeding process, molecular marker-assisted selection was carried out using badh2-E2 and Wxmp, and seed selection was conducted based on field performance. The line with the most favorable comprehensive traits was chosen from the stable lines of F8 and designated as Hu 12-30. Subsequently, Xiushui 114, a blast-resistant variety suitable for direct seeding and carrying the Pita and Pik-m genes, was used as the female parent, and Hu 12-30 as the male parent for cross-breeding. In the course of backcrossing and self-crossing, molecular marker-assisted selection was performed using badh2-E2, Wxmp, Pita, and Pik-m. By integrating quality determination, blast resistance identification, and identification of suitability for direct seeding, a new high-quality and resistant japonica rice variety, Huxianggeng 216, was developed. 【Result】Huxianggeng 216 contains the fragrance gene badh2-E2, the low-amylose-content gene Wxmp, and the blast-resistance genes Pita and Pik-m. This variety has a fragrant aroma, a soft and elastic texture, with the amylose content ranging from 9.2% to 10.4%. The comprehensive resistance index for rice blast is 2.5, and the disease grade is 3, indicating moderate resistance. The resistance grade for rice stripe leaf blight is 3, showing resistance. Huxianggeng 216 has a high direct-seeding rate, with a plant height of 87 cm. It has strong lodging resistance and a yield of 650 kg per 667 m².【Conclusion】Molecular marker-assisted selection combined with phenotypic identification of target traits is an important means to achieve multi-target trait aggregation breeding, which can accurately and effectively develop high-quality multi-resistant japonica rice varieties.
YAN Ying, WANG Kai, ZHANG Lixia, HU Zejun, YE Junhua, YANG Hang, GU Chunjun, WU Shujun . Development of a New High-Quality and Multi-Resistant japonica Rice Variety, Huxianggeng 216, Through Molecular Pyramiding Breeding[J]. Chinese Journal OF Rice Science, 2025 , 39(2) : 209 -219 . DOI: 10.16819/j.1001-7216.2025.240204
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