Chinese Journal OF Rice Science >
Sequence Variation Analysis of the Parthenogeny-inducing Gene BBM1 in Rice
Received date: 2023-12-10
Revised date: 2024-01-10
Online published: 2024-11-15
【Objective】Ectopic expression of BBM1 in rice egg cells can induce parthenogenesis. Exploring superior allelic variants of BBM1 and functionally similar genes is of great significance for heterosis fixation in rice.【Method】By utilizing third-generation sequencing data from 383 core rice accessions, we conducted an analysis of structural variations, sequence polymorphisms, and haplotypes of BBM1. Additionally, we screened for genes with similar sequences and expression patterns to identify functional analogues of BBM1.【Results】Our findings revealed the presence of two copies of the BBM1 gene in three rice accessions, along with MITEs insertion variants observed in two O. rufipogon accessions. The BBM1 sequence exhibited a clear indica-japonica differentiation, with 94.80% of japonica accessions and 3.80% of indica accessions experiencing premature termination due to single base mutations, resulting in the loss of 26 amino acid residues. Furthermore, the coding region of BBM1 is highly conserved, and most rice accessions share the same haplotype. We also identified LOC_Os06g44750 as a candidate gene with a similar sequence and expression pattern to BBM1, which may potentially contribute to inducing parthenogenesis.【Conclusion】This study provides insights into the variation present within the BBM1 gene in rice while also identifying important haplotypes and potential candidate genes with functional similarities. This foundational knowledge serves to enhance the frequency of parthenogenesis in rice.
Key words： rice; BBM1; parthenogenesis; sequence variation
WANG Qing, WANG Yanru, ZHANG Xiuli, LÜ Qiming . Sequence Variation Analysis of the Parthenogeny-inducing Gene BBM1 in Rice[J]. Chinese Journal OF Rice Science, 2024 , 38(6) : 627 -637 . DOI: 10.16819/j.1001-7216.2024.231210
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