Chinese Journal OF Rice Science >
Investigation and Species Identification of Cyst Nematode Disease on Rice in Guangdong Province
Received date: 2023-10-27
Revised date: 2024-02-03
Online published: 2024-07-12
【Objective】 To investigate the species, distribution, and occurrence of cyst nematode disease on rice in Guangdong Province. 【Methods】 A total of 231 rice roots and rhizosphere soil samples from 14 cities in Guangdong Province were collected and analyzed. The cyst nematode species in these samples were identified through morphological and molecular identification. 【Results】 Cyst nematodes were found on rice in Qingyuan, Zhaoqing, and Jiangmen in Guangdong Province, with detection rates of 40%, 23.7%, and 10%, respectively. Morphological identification revealed that the cysts were lemon to spherical-shaped, predominantly lemon-shaped, with an ambifenestrate vulval cone, a nearly round fenestral, a narrow vulval bridge, and a medium-sized underbridge. The second-stage juvenile (J2) had a hemispherical and offset labial region, a conical tail with a pointed tip, and a hyaline region occupying 53% to 66% of the tail length. Molecular identification indicated that the 28S rDNA D2-D3 region of the cyst nematode was 99.73%－100% similar to Heterodera elachista in the NCBI database, and the rDNA-ITS region was 96.72%－99.91% similar to Heterodera elachista in the NCBI database. Phylogenetic analysis placed the nematode in the same branch as H. elachista with a confidence level of 100%. Using specific primers He-F/He-R of H. elachista, a 281 bp single band was amplified from the rDNA-ITS region of the cyst nematodes, consistent with previous reports. 【Conclusion】 The cyst nematodes collected in Qingyuan, Zhaoqing, and Jiangmen were identified as H. elachista, marking the first report of H. elachista in Guangdong Province.
LIU Fuxiang, ZHEN Haoyang, PENG Huan, ZHENG Liuchun, PENG Deliang, WEN Yanhua . Investigation and Species Identification of Cyst Nematode Disease on Rice in Guangdong Province[J]. Chinese Journal OF Rice Science, 2024 , 38(4) : 456 -461 . DOI: 10.16819/j.1001-7216.2024.231001
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