Chinese Journal OF Rice Science >
QTL Mapping for Tiller Angle in Rice by Genome-wide Association Analysis
Received date: 2023-09-12
Revised date: 2023-12-29
Online published: 2024-05-13
【Objective】Tiller angle is a critical agronomic trait influencing rice yield. Identifying rice tiller angle QTL (genes) and detecting their elite haplotypes can be beneficial for developing ideal rice varieties. 【Method】333 core germplasms from the rice 3K resources were utilized as research materials. These germplasms were cultivated in Yunyuan and Chunhua of Hunan Agricultural University in 2020 and 2022, respectively. Tiller angles of various germplasms were measured during the heading stage. Genome-wide association analysis was conducted using the MLM model of TASSEL 5.2, combined with the genotypes of the germplasms. 【Results】Six QTL for tiller angle were identified on rice chromosomes 2, 5, 6, 9, and 12, designated as qTA2, qTA5, qTA6.1, qTA6.2, qTA9, and qTA12, respectively. These QTL explained phenotypic variation ranging from 6.23% to 16.22%. Notably, qTA9 co-localized with the major QTL TAC1 for tiller angle, while the other five QTL were newly discovered. Candidate gene analysis was conducted for these five QTL. The candidate genes for qTA2 and qTA6.1 were identified as Os02g0817900 and Os06g0682800, respectively. Os02g0817900 encodes a rice cytochrome P450 family protein, while Os06g0682800 encodes a zinc finger domain protein.【Conclusion】This study successfully identified new QTL for tiller angle in rice and analyzed candidate genes, offering valuable insights for the cloning of tiller angle QTL (genes) and genetic improvement of tiller angle in rice.
Key words： rice (Oryza sativa L.); tiller angle; QTL; candidate gene; haplotype
ZHU Yujing, GUI Jinxin, GONG Chengyun, LUO Xinyang, SHI Jubin, ZHANG Haiqing, HE Jiwai . QTL Mapping for Tiller Angle in Rice by Genome-wide Association Analysis[J]. Chinese Journal OF Rice Science, 2024 , 38(3) : 266 -276 . DOI: 10.16819/j.1001-7216.2024.230904
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