Chinese Journal OF Rice Science >
Genome-wide Association Analysis of Rice Seed Dehydration Rate at Maturity Stage
Received date: 2023-03-17
Revised date: 2023-06-16
Online published: 2024-03-14
【Objective】The dehydration rate of grains directly influences the safe harvesting and rapid drying of seeds. Therefore, to ensure seed quality and reduce production costs, varieties with low grain moisture content and fast dehydration rates were selected for production. 【Method】A total of 165 rice core germplasms from 82 different countries and regions were used as experimental materials. Genome-wide association analysis (GWAS) was conducted by combining the phenotype and genotype of seed dehydration rates at the maturity stage. This aimed to explore key genes sensitive to dehydration and their regulatory roles in rice germplasm, laying a foundation for cultivating and developing fast dehydration varieties. 【Results】1) Descriptive statistical analysis of dehydration rate traits of the rice core germplasm population showed that these traits exhibited continuously skewed normal distributions over two years, with significant correlations between years for rapid dehydration rates. Rapid dehydration rates were positively correlated with slow dehydration rates across different subgroups. 2) GWAS analysis identified 170 SNPs and 36 QTLs associated with dehydration rates. LD analysis revealed six QTLs closely related to dehydration rates: qGDR2.3, qGDR4.1, qGDR4.2, qGDR6.1, qGDR6.4, and qGDR10.1. 【Conclusion】 The main candidate genes within these QTL intervals, including OsPIP1;1, OsTIFY9, OsbZIP48, OsATG8b, OsDREB1C, OsSCP46, are closely associated with water transport activity, signal transduction, transcriptional regulation, and antioxidant defense. It is speculated that they play roles in seed dehydration rates and can serve as optimal candidate genes.
Key words： rice seed; dehydration rate; QTL; genome-wide association analysis
LIU Zhongqi, ZHANG Haiqing, HE Jiwai, GUI Jinxin . Genome-wide Association Analysis of Rice Seed Dehydration Rate at Maturity Stage[J]. Chinese Journal OF Rice Science, 2024 , 38(2) : 150 -159 . DOI: 10.16819/j.1001-7216.2024.230305
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