【Objective】 Rice borers are widely distributed in Guangxi and pose a serious threat to rice production. The aim of this study is to clarify the overwintering situation and spatial distribution pattern of rice stem borers in Guangxi, understand the distribution and species of rice stem borers, and provide a basis for prediction and scientific control. 【Method】 Rice stem borers were investigated in 27 cities (counties) in the northeast, northwest, southeast, southwest, and coastal areas of Guangxi, respectively. The checkerboard sampling method was employed to investigate the overwintering borers. Rice stubbles were collected and brought back to the laboratory for careful species identification and quantification of borers. The spatial distribution pattern of the borer population was analyzed. 【Result】 The results of the overwintering survey revealed that the overwintering borer species in our region included Sesamia inferens, Chilo suppressalis, Scirpophaga incertulas, and Chilotraea auricilia. Among them, the overwintering population was primarily composed of S. inferens and C. suppressalis, with S. incertulas occurring mainly in the latitude of 23°－25°. The overwintering population density of other borers did not exhibit an obvious regular distribution in our region. Spatial distribution parameters indicated that borers in other fields displayed an aggregation distribution, with the degree of aggregation increasing with density, except for S. inferens in fields 13, 20, and 22, where they were uniformly distributed. The aggregation of borers in paddy field was mostly attributed to environmental factors. 【Conclusion】 In Guangxi, overwintering borers are clustered, with dominant populations of S. inferens and C. suppressalis in the field, followed by C. auricilia, while S. incertulas occurred sporadically.