Chinese Journal OF Rice Science >
Morphological and Physiological Characteristics of Rice Roots Under Combined Salinity-Drought Stress and Their Relationships with Yield Formation
Received date: 2023-05-12
Revised date: 2023-09-05
Online published: 2023-11-14
【Objective】 This study aims to elucidate the mechanisms by which combined salinity-drought stress affects rice root morphology, physiology, and yield formation. 【Method】 We used Nanjing 9108, a conventional japonica rice variety widely grown in the mudflats along the coast of Jiangsu Province, as the test material. The following treatments were applied: control (no stress, CK), single salinity stress (0.15s, salinity concentration 0.15%; 0.3s, salinity concentration 0.3%), single drought stress (DJ, drought at jointing stage; DH, drought at heading stage), and combined salinity-drought stress (0.15s+DJ, 0.15s+DH, 0.3s+DJ, 0.3s+DH). We conducted a comparative study to investigate changes in root morphology and physiology of rice under combined salinity-drought stress and their underlying relationships with crop production and yield formation. 【Results】 In comparison to the control, rice yield decreased by 25.8% and 65.0% under single salinity stress (0.15s and 0.3s, respectively). Under single drought stress (DJ and DH), the yield reduction was 4.3% and 22.3%, respectively. Under combined salinity-drought stress (0.15s+DJ, 0.3s+DJ, 0.15s+DH, and 0.3s+DH), the yield reduction was 33.3%, 67.3%, 48.3%, and 72.6%, respectively. The number of panicles, grain number per panicle, seed setting rate, and thousand grain weight were significantly lower under single salinity stress and combined salinity-drought stress treatments compared to the control. The yield components of rice under combined salinity-drought stress were also significantly lower than those under single salinity stress and drought stress. Aboveground dry matter weight and harvest index of rice during maturity were lower under single salinity stress, drought stress, and combined salinity-drought stress compared to the control. The aboveground and root dry matter weight, root to shoot ratio, and harvest index of plants under combined salinity-drought stress were lower than those under single salinity stress and drought stress. Root length, root surface area, root diameter, root oxidation activity, and bleeding intensity were significantly inhibited under single salinity stress and combined salinity-drought stress, with a stronger inhibitory effect under combined salinity-drought stress. Net photosynthetic rate and chlorophyll contents of rice leaves were reduced under single salinity stress, drought stress, and combined salinity-drought stress, with the greatest decrease observed under combined salinity-drought stress. 【Conclusion】Combined salinity-drought stress significantly inhibits rice root morphology and physiology, consequently impacting leaf photosynthesis, assimilate accumulation, and yield formation. This inhibitory effect is more pronounced than that of single salinity stress and drought stress, with a synergistic impact.
ZHU Wang, ZHANG Xiang, GENG Xiaoyu, ZHANG Zhe, CHEN Yinglong, WEI Huanhe, DAI Qigen, XU Ke, ZHU Guanglong, ZHOU Guisheng, MENG Tianyao . Morphological and Physiological Characteristics of Rice Roots Under Combined Salinity-Drought Stress and Their Relationships with Yield Formation[J]. Chinese Journal OF Rice Science, 2023 , 37(6) : 617 -627 . DOI: 10.16819/j.1001-7216.2023.230504
| [1] | Ma Y, Dias M C, Freitas H. Drought and salt stress responses and microbe-induced tolerance in plants[J]. Frontiers in Plant Science, 2020, 11: 591911. |
| [2] | Meng T Y, Zhang X B, Ge J L, Chen X, Yang Y L, Zhu G L, Chen Y L, Zhou G S, Wei H H, Dai Q G. Agronomic and physiological traits facilitating better yield performance of japonica/indica hybrids in saline fields[J]. Field Crops Research, 2021, 271: 108255. |
| [3] | Chen Y, Li S Y, Zhang Y J, Li T T, Ge H M, Xia S M, Gu J F, Zhang H. Rice root morphological and physiological traits interaction with rhizosphere soil and its effect on methane emissions in paddy fields[J]. Soil Biology and Biochemistry, 2019, 129(18): 191-200. |
| [4] | Liu Q, Liang Z, Feng D, Jiang S J, Wang Y F, Du Z Y, Li R X, Hu G H. Transcriptional landscape of rice roots at the single-cell resolution[J]. Plant Molecular Biology, 2021, 14(3): 384-394. |
| [5] | Xu N, Chu Y L, Chen H L, Li X X, Wu Q, Jin L, Wang G X, Huang J L. Rice transcription factor OsMADS25 modulates root growth and confers salinity tolerance via the ABA-mediated regulatory pathway and ROS scavenging[J]. PLoS Genetics, 2018, 14(10): e1007662. |
| [6] | Hussain S, Zhang J H, Zhong C, Zhu L F, Cao X C, Yu S M, James A B, Hu J J, Jin Q Y. Effects of salt stress on rice growth development characteristics and the regulating ways: A review[J]. Journal of Integrative Agriculture, 2017, 16(11): 2357-2374. |
| [7] | Chen T X, Shabala S, Niu Y N, Chen Z H, Shabala L, Meinke H, Venkataraman G, Pareek A, Xu J L, Zhou M X. Molecular mechanisms of salinity tolerance in rice[J]. The Crop Journal, 2021, 9(3): 506-520. |
| [8] | 王洋, 张瑞, 刘永昊, 李荣凯, 葛建飞, 邓仕文, 张徐彬, 陈英龙, 韦还和, 戴其根. 水稻对盐胁迫的响应及耐盐机理研究进展[J]. 中国水稻科学, 2022, 36(2): 105-117. |
| [8] | Wang Y, Zhang R, Liu Y H, Li R K, Ge T F, Deng S W, Zhang X B, Chen Y L, Wei H H, Dai Q G. Research progress on response and salinity tolerance mechanism of rice to salt stress[J]. Chinese Journal of Rice Science, 2022, 36(2): 105-117. (in Chinese with English abstract) |
| [9] | 韦还和, 张徐彬, 葛佳琳, 陈熙, 孟天瑶, 杨洋, 熊飞, 陈英龙, 戴其根. 盐胁迫对水稻颖花形成及籽粒充实的影响[J]. 作物学报, 2021, 47(12): 2471-2480. |
| [9] | Wei H H, Zhang X B, Ge J L, Chen X, Meng T Y, Yang Y, Xiong F, Chen Y L, Dai Q G. Effects of salinity stress on spikelet formation and grain filling in rice[J]. Acta Agronomica Sinica, 2021, 47(12): 2471-2480. (in Chinese with English abstract) |
| [10] | 李婷婷, 冯钰枫, 朱安, 黄健, 汪浩, 李思宇, 刘昆, 彭如梦, 张宏路, 刘立军. 主要节水灌溉方式对水稻根系形态生理的影响[J]. 中国水稻科学, 2019, 33(4): 293-302. |
| [10] | Li T T, Feng Y F, Zhu A, Huang J, Wang H, Li S Y, Liu K, Peng R M, Zhang H L, Liu L J. Effects of main water-saving irrigation methods on rice root morphology and physiology[J]. Chinese Journal of Rice Science, 2019, 33(4): 293-302. (in Chinese with English abstract) |
| [11] | 刘亚文, 张红燕, 曹丹, 李兰芝. 基于多平台基因表达数据的水稻干旱和盐胁迫相关基因预测[J]. 作物学报, 2021, 47(12): 2423-2439. |
| [11] | Liu Y W, Zhang H Y, Cao D, Li D Z. Prediction of drought and salt stress related genes in rice based on multi-platform gene expression data[J]. Acta Agronomica Sinica, 2021, 47(12): 2423-2439. (in Chinese with English abstract) |
| [12] | 翁亚伟. 小麦幼苗对盐旱复合胁迫的响应机理[D]. 南京: 南京农业大学, 2016. |
| [12] | Weng Y W. Response mechanism of wheat seedlings to salt and drought stress[D]. Nanjing: Nanjing Agricultural University, 2016. (in Chinese with English abstract) |
| [13] | 高荣嵘, 杨莎, 郭峰, 张佳蕾, 耿耘, 崔利, 孟静静, 李新国, 万书波. 盐旱交叉胁迫对花生生长发育和生理特性的影响[J]. 中国油料作物学报, 2018, 40(2): 218-226. |
| [13] | Gao R Z, Yang S, Guo F, Zhang J L, Geng Y, Cui L, Meng J J, Li X G, Wan S B. Effects of salt and drought stress on growth and physiological characteristics of Peanut[J]. Chinese Journal of Oil Crop Sciences, 2018, 40(2): 218-226. (in Chinese with English abstract) |
| [14] | 肖德顺, 徐春梅, 王丹英, 章秀福, 陈松, 褚光, 刘元辉. 根际氧环境对水稻幼苗根系微形态结构的影响及其生理机制[J]. 中国水稻科学, 2022, 36(4): 399-409. |
| [14] | Xiao D S, Xu C M, Wang D Y, Zhang X F, Chen S, Chu G, Liu Y H. Effect of rhizosphere oxygen environment on root micromorphology of rice seedlings and its physiological mechanism[J]. Chinese Journal of Rice Science, 2022, 36(4): 399-409. (in Chinese with English abstract) |
| [15] | 张晨晖, 章岩, 李国辉, 杨子君, 查莹莹, 周驰燕, 许轲, 霍中洋, 戴其根, 郭保卫. 侧深施肥下水稻高产形成的根系形态及其生理变化特征[J]. 作物学报, 2023, 49(4): 1039-1051. |
| [15] | Zhang C H, Zhang Y, Li G H, Yang Z J, Zha Y Y, Zhou C Y, Xu K, Huo Z Y, Dai Q G, Guo B W. Root morphology and physiological characteristics of rice high yield formation under lateral deep fertilization[J]. Acta Agronomica Sinica, 2023, 49(4): 1039-1051. (in Chinese with English abstract) |
| [16] | 景文疆, 顾汉柱, 张小祥, 吴昊, 张伟杨, 顾骏飞, 刘立军, 王志琴, 杨建昌, 张耗. 中籼水稻品种改良过程中米质和根系特征对灌溉方式的响应[J]. 中国水稻科学, 2022, 36(5): 505-519. |
| [16] | Jing W J, Gu H Z, Zhang X X, Wu H, Zhang W Y, Gu J F, Liu L J, Wang Z Q, Yang J C, Zhang H. Response of rice quality and root characteristics to irrigation methods during the improvement of medium indica rice varieties[J]. Chinese Journal of Rice Science, 2022, 36(5): 505-519. (in Chinese with English abstract) |
| [17] | 印玉明, 王永清, 马春晨, 郑恒彪, 程涛, 田永超, 朱艳, 曹卫星, 姚霞. 利用日光诱导叶绿素荧光监测水稻叶片叶绿素含量[J]. 农业工程学报, 2021, 37(12): 169-180. |
| [17] | Yin Y M, Wang Y Q, Ma C C, Zheng H B, Cheng T, Tian Y C, Zhu Y, Cao W X, Yao X. Monitoring chlorophyll content in rice leaves by solar-induced chlorophyll fluorescence[J]. Transaction of the Chinese Society of Agricultural Engineering, 2021, 37(12): 169-180. (in Chinese with English abstract) |
| [18] | 黄露, 余明龙, 冯乃杰, 郑殿峰, 马国辉. 14-羟芸·噻苯隆对盐胁迫下水稻光合特性、抗氧化系统及产量的影响[J]. 生态学杂志, 2023, 42(3): 668-676. |
| [18] | Huang L, Yu M L, Feng N J, Zheng D J, Ma G H. Effects of 14-hydroxy rutin·thiaphenuron on photosynthetic characteristics, antioxidant system and yield of rice under salinity stress[J]. Journal of Ecology, 2023, 42(3): 668-676. (in Chinese with English abstract) |
| [19] | 谢华英, 马均, 代邹, 李玥, 孙加威, 赵建红, 徐徽, 孙永健. 抽穗期高温干旱胁迫对杂交水稻产量及生理特性的影响[J]. 杂交水稻, 2016, 31(1): 62-148. |
| [19] | Xie H Y, Ma J, Dai Z, Li Y, Shun J W, Zhao J H, Xu H, Shun Y J. Effects of high temperature and drought stress on yield and physiological characteristics of hybrid rice at heading stage[J]. Hybrid Rice, 2016, 31(1): 62-148. (in Chinese with English abstract) |
| [20] | 杨晓龙, 程建平, 汪本福, 李阳, 张枝盛, 李进兰, 李萍. 灌浆期干旱胁迫对水稻生理性状和产量的影响[J]. 中国水稻科学, 2021, 35(1): 38-46. |
| [20] | Yang X L, Cheng J P, Wang B F, Li Y, Zhang Z S, Li J L, Li P. Effects of drought stress on physiological characters and yield of rice during grain-filling period[J]. Chinese Journal of Rice Science, 2021, 35(1): 38-46. (in Chinese with English abstract) |
| [21] | 邵靖宜, 李小凡, 于维祯, 刘鹏, 赵斌, 张吉旺, 任佰朝. 高温干旱复合胁迫对夏玉米产量和茎秆显微结构的影响[J]. 中国农业科学, 2021, 54(17): 3623-3631. |
| [21] | Shao J Y, Li X F, Yu W Z, Li P, Zhao B, Zhang J W, Ren B C. Effects of combined high temperature and drought stress on the yield and stem microstructure of summer maize[J]. Scientia Agricultura Sinica, 2021, 54(17): 3623-3631. (in Chinese with English abstract) |
| [22] | 魏晓东, 张亚东, 宋雪梅, 陈涛, 朱镇, 赵庆勇, 赵凌, 路凯, 谢寅峰, 王才林. 超级稻品种南粳5718高产的光合生理特性研究[J]. 作物学报, 2022, 48(11): 2879-2890. |
| [22] | Wei X D, Zhang Y D, Song X M, Chen T, Zhu Z, Zhao Q Y, Zhao L, Lu K, Xie J F, Wang C L. Study on the photosynthetic physiological characteristics of high yield of super rice variety Nanjing 5718[J]. Acta Agronomica Sinica, 2022, 48(11): 2879-2890. (in Chinese with English abstract) |
| [23] | 赵凌, 张勇, 魏晓东, 梁文化, 赵春芳, 周丽慧, 姚姝, 王才林, 张亚东. 利用高密度Bin图谱定位水稻抽穗期剑叶叶绿素含量QTL[J]. 中国农业科学, 2022, 55(5): 825-836. |
| [23] | Zhao L, Zhang Y, Wei X D, Liang W H, Zhao C F, Zhou L H, Yao S, Wang C L, Zhang Y D. Mapping the QTL of chlorophyll content in flag leaves of rice at heading stage using high-density Bin maps[J]. Scientia Agricultura Sinica, 2022, 55(5): 825-836. (in Chinese and English abstract) |
| [24] | 谷娇娇, 胡博文, 贾琰, 沙汉景, 李经纬, 马超, 赵宏伟. 盐胁迫对水稻根系相关性状及产量的影响[J]. 作物杂志, 2019(4): 176-182. |
| [24] | Gu J J, Hu B W, Jia Y, Sha H J, Li J W, Ma C, Zhao H W. Effects of salinity stress on root related traits and yield of rice[J]. Crops, 2019(4): 176-182. (in Chinese with English abstract) |
| [25] | 段素梅, 杨安中, 黄义德, 吴文革, 许有尊, 陈刚. 干旱胁迫对水稻生长、生理特性和产量的影响[J]. 核农学报, 2014, 28(6): 1124-1132. |
| [25] | Duan S M, Yang A Z, Huang Y D, Wu W G, Xu Y Z, Chen G. Effects of drought stress on growth physiological characteristics and yield of rice[J]. Journal of Nuclear Agricultural Sciences, 2014, 28(6): 1124-1132. (in Chinese with English abstract) |
| [26] | 吴学祝, 蔡昆争, 骆世明. 抽穗期土壤干旱对水稻根系和叶片生理特性的影响[J]. 中国农学通报, 2008(7): 202-207. |
| [26] | Wu X Z, Cai K Z, Luo S M. Effects of soil drought at heading stage on physiological characteristics of rice roots and leaves[J]. Chinese Agricultural Science Bulletin, 2008(7): 202-207. (in Chinese with English abstract) |
| [27] | 明东风, 袁红梅, 王玉海, 宫海军, 周伟军. 水分胁迫下硅对水稻苗期根系生理生化性状的影响[J]. 中国农业科学, 2012, 45(12): 2510-2519. |
| [27] | Ming D F, Yuan K Z, Wang H Y, Gong H J, Zhou W J. Effect of silicon on physiological and biochemical characters of rice root system during seedling stage under water stress[J]. Scientia Agricultura Sinica, 2012, 45(12): 2510-2519. (in Chinese with English abstract) |
| [28] | 补红英, 宋维周, 曹凑贵, 李萍. 节水抗旱稻旱优113号的根系生长对土壤水分亏缺的响应[J]. 中国农业科学, 2017, 50(22): 4277- 4289. |
| [28] | Bu H Y, Song W Z, Cao C G, Li P. Response of root growth to soil water deficit of water-saving and drought-resistant rice Hanyou 113[J]. Scientia Agricultura Sinica, 2017, 50(22): 4277-4289. (in Chinese with English abstract) |
| [29] | 吴文超, 曲延英, 高文伟, 吴鹏昊, 陈全家. 不同棉花品种对盐、旱胁迫的光合响应及抗逆性评价[J]. 新疆农业科学, 2016, 53(9): 1569-1579. |
| [29] | Wu W C, Qu Y Y, Gao W W, Wu P H, Chen Q J. Photosynthetic response and stress resistance evaluation of different cotton varieties under salt and drought stress[J]. Xinjiang Agricultural Sciences, 2016, 53(9): 1569-1579. (in Chinese with English abstract) |
| [30] | 翁亚伟, 张磊, 张姗, 田中伟, 靳雪莹, 李梦雅, 余钟毓, 姜东, 戴廷波. 盐旱复合胁迫对小麦幼苗生长和水分吸收的影响[J]. 生态学报, 2017, 37(7): 2244-2252. |
| [30] | Weng Y W, Zhang L, Zhang S, Tian Z W, Jin X Y, Li M Y, Yu Z Y, Jiang D, Dai T B. Effects of salinity and drought stress on growth and water absorption of wheat seedlings[J]. Acta Ecologica Sinica, 2017, 37(7): 2244-2252. (in Chinese with English abstract) |
| [31] | 董立强, 杨铁鑫, 李睿, 商文奇, 马亮, 李跃东, 隋国民. 株行距配置对超高产田水稻产量及根系形态生理特性的影响[J]. 中国水稻科学, 2023, 37(4): 392-404. |
| [31] | Dong L Q, Yang T X, Li R, Shang W Q, Ma L, Li Y D, Sui G M. Effects of plant row spacing configuration on rice yield and root morphological and physiological characteristics in super high yield fields[J]. Chinese Journal of Rice Science, 2023, 37(4): 392-404. (in Chinese with English abstract) |
| [32] | 张桂云, 朱静雯, 孙明法, 严国红, 刘凯, 宛柏杰, 代金英. 盐胁迫条件下长白10号水稻籽粒中差异代谢物的分析[J]. 中国农业科学, 2021, 54(4): 675-683. |
| [32] | Zhang G Y, Zhu J W, Sun M Y, Yan G H, Liu K, Wan B J, Dai Y J. Analysis of differential metabolites in Changbai 10 rice grains under salt stress conditions[J]. Scientia Agricultura Sinica, 2021, 54(4): 675-683. (in Chinese with English abstract) |
| [33] | 付景, 王亚, 杨文博, 王越涛, 李本银, 王付华, 王生轩, 白涛, 尹海庆. 干湿交替灌溉耦合施氮量对水稻籽粒灌浆生理和根系生理的影响[J]. 作物学报, 2023, 49(3): 808-820. |
| [33] | Fu J, Wang Y, Yang W B, Wang Y T, Li B Y, Wang F H, Wang S S, Bai T, Yin H Q. Effects of different emergence time and density of barnyard grass on root characteristics of mechanically direct seeding rice at filling stage[J]. Acta Agronomica Sinica, 2023, 49(3): 808-820. (in Chinese with English abstract) |
| [34] | 段骅, 唐琪, 剧成欣, 刘立军, 杨建昌. 抽穗灌浆早期高温与干旱对不同水稻品种产量和品质的影响[J]. 中国农业科学, 2012, 45(22):4561-4573. |
| [34] | Du H, Tang Q, Ju C Y, Liu L J, Yang J C. The effects of high temperature and drought in the early stage of heading and filling on the yield and quality of different rice varieties[J]. Scientia Agricultura Sinica, 2012, 45(22): 4561-4573. (in Chinese with English abstract) |
/
| 〈 |
|
〉 |