Chinese Journal OF Rice Science >
Development and Agronomic Traits Analysis of New Rice Resistance Lines to Xanthomonas oryzae pv. oryzicola
Received date: 2023-04-20
Revised date: 2023-05-22
Online published: 2023-11-14
【Objective】 This study aims to develop new rice resistance lines to bacterial leaf streak (BLS) and provide core germplasm materials for BLS resistance breeding. 【Method】 BHADOIA303 harboring the Xo2 gene from Bangladesh was used as the BLS resistance donor, and an elite indica variety Wushansimiao was chosen as the recipient, to develop resistant lines by advanced backcrossing, molecular marker-assisted selection and GSR40K chip detection. The BLS resistance lines was evaluated by artificial inoculation with pathogen inoculum spraying and syringe infiltration in the field, and their agronomic traits were evaluated synchronously. 【Result】 Molecular markers and GSR40K microarray detection confirmed the presence of the target gene Xo2 in 10 new lines (BC4F5). Significant differences were observed in the BLS resistance between the introgression lines and Wushansimiao, no matter whether they were inoculated by spraying or infiltration. The average disease index ranged from 5.00 to 10.37, with an average lesion length of 0.70 to 0.99 cm. Lines 1-3 showed the best resistance. The agronomic and yield traits of the 3 lines with better resistance were comparable to those of Wushansimiao. The 1000-grain weight, grain length and width of line 1 were similar to those of Wushansimiao, and its seed setting rate was better than that of Wushansimiao. Number of total grains per panicle of Line 2 was similar to that of Wushansimiao with longer and wider grains, and a higher 1000-grain weight. The 1000-grain weight and seed setting rate of Line 3 were similar to those of Wushansimiao, and the number of panicles per plant was better than that of Wushansimiao. 【Conclusion】 Three Xo2 resistance lines with better agronomic traits and greatly enhanced resistance to BLS were obtained, which would provide resistance elite germplasm materials and accelerate the process of BLS resistance molecular breeding.
FENG Aiqing, WANG Congying, SU Jing, FENG Jinqi, CHEN Kailing, LIN Xiaopeng, CHEN Bing, LIANG Meiling, YANG Jianyuan, ZHU Xiaoyuan, CHEN Shen . Development and Agronomic Traits Analysis of New Rice Resistance Lines to Xanthomonas oryzae pv. oryzicola[J]. Chinese Journal OF Rice Science, 2023 , 37(6) : 587 -596 . DOI: 10.16819/j.1001-7216.2023.230406
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