Chinese Journal OF Rice Science >
cDNA Cloning and Molecular Characterization of OsTAF12b Gene in Oryza sativa
Received date: 2023-01-28
Revised date: 2023-03-10
Online published: 2023-11-14
【Objective】 In order to determine the alternative splicing forms of TATA-box binding protein (TBP) associated factor 12b (OsTAF12b), a component of the general transcription factor ⅡD (TFⅡD), in rice (Oryza sativa), characterize their subcellular localization and expression patterns, and provide basal information for further functional studies. 【Method】 The full-length cDNA of OsTAF12b was amplified and cloned by rapid amplification of cDNA 5’-/3’-ends (RACE). Multiple sequence alignment and a phylogenetic tree were conducted by bioinformatic analysis. The subcellular localization of OsTAF12b was observed with a laser confocal microscopy and its expression patterns under abiotic stresses were analyzed by qRT-PCR. 【Results】 Four alternative splicing forms of OsTAF12b were identified and there was only one lysine difference within their coding regions. OsTAF12b was highly homologous to those of other gramineous plants and they were grouped together in a clade in the phylogenetic tree. The GFP-fused OsTAF12b protein was colocalized with H2B, a marker labeling nuclear localization, in both cells of N. benthamiana leaves and rice protoplasts. Its transcript level was higher in leaves and significantly up-regulated when exposed to different abiotic stresses. 【Conclusion】 The OsTAF12b gene has four alternative splicing forms of transcripts and encodes two nuclear proteins with only a single lysine residue difference. Its expression patterns suggest that OsTAF12 could be involved in response to a variety of abiotic stresses.
QI Panpan, GUO Liuming, LI Jing, LÜ Mingfang, YUAN Zhengjie, ZHANG Hengmu . cDNA Cloning and Molecular Characterization of OsTAF12b Gene in Oryza sativa[J]. Chinese Journal OF Rice Science, 2023 , 37(6) : 577 -586 . DOI: 10.16819/j.1001-7216.2023.230108
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