Chinese Journal OF Rice Science >
Effects of Exogenous Trehalose on Grain Filling Characteristics and Yield Formation of japonica Rice Cultivar W1844
Received date: 2022-07-29
Revised date: 2023-01-26
Online published: 2023-07-17
【Objective】 It is very important to clarify the effect of exogenous trehalose on grain filling and seed setting of large-panicle japonica rice. 【Method】 The effects of different concentrations of trehalose on photosynthetic characteristics, dry matter accumulation and translocation of stems and sheath, grain filling characteristics and yield of large-panicle japonica rice W1844 were investigated by foliar spraying of various concentrations of trehalose (T0, T15, T30, T50, T70 and T100, 0, 15, 30, 50, 70, 100mmol/L, respectively) at the end of booting stage. 【Result】 1) Trehalose exposure increased grain yield, and the highest yield was obtained under T50 treatment. Compared with T0, the yield under T50 treatment increased by 7.71% on average, and the yield and thousand grain weight of superior spikelets increased by 4.36% and 5.92%, respectively, while the yield and thousand grain weight of inferior spikelets increased by 11.98% and 10.01%, respectively. Trehalose had a better effect on the improvement of grain filling and seed-setting of the inferior spikelets. 2) Trehalose treatment changed the grain filling characteristics. Compared with T0, the time to the maximum filling rate of superior and inferior spikelets was shortened by 3.70 and 3.93 d under T50 treatment; the average filling rates of superior and inferior spikelets were increased by 16.57% and 28.03% in the early stage of filling; the average filling rates of superior and inferior spikelets were increased by 20.74% and 24.54% in the middle of grain filling. In addition, compared with T0, the grain weight of superior and inferior spikelets at maximum filling rate increased by 6.74% and 7.36%, respectively. The net photosynthetic rate, stomatal conductance and transpiration rate of rice leaves under T50 treatment increased by 31.08%, 42.58% and 10.42% on average, respectively as compared with T0. 4) Trehalose treatment promoted the accumulation and translocation of non-structural carbohydrates (NSC) in stem and sheath during the heading stage. Compared with T0, the NSC content in stem and sheath at the heading stage increased by 11.63% on average under T50 treatment, and the translocation of dry matter in stems and sheaths increased by 28.52% on average, and the contribution rate to seeds increased by 18.18%. This might be due to the up-regulated expression level of sucrose transporter genes OsSUT2, OsSUT4 and OsSUT5 in stems and sheaths, which promoted dry matter transport in stems and sheaths.【Conclusion】 Exogenous trehalose could effectively improve grain filling and seed setting of large-panicle japonica rice, which might be due to the fact that trehalose treatment could increase the net photosynthetic rate of flag leaf, up-regulate the expression of sucrose transporter genes, and promote the accumulation and translocation of NSC in the stems and sheaths, thus increasing the grain filling rate, seed-setting rate and grain weight of superior and inferior grains. Under the present experimental conditions, 50 mmol/L is the most effective in improving grain filling and seed setting in large-panicle rice W1844.
HUANG Yaru, XU Peng, WANG Lele, HE Yizhe, WANG Hui, KE Jian, HE Haibing, WU Liquan, YOU Cuicui . Effects of Exogenous Trehalose on Grain Filling Characteristics and Yield Formation of japonica Rice Cultivar W1844[J]. Chinese Journal OF Rice Science, 2023 , 37(4) : 379 -391 . DOI: 10.16819/j.1001-7216.2023.220712
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