Chinese Journal OF Rice Science >
Phenotypic Analysis and Fine Mapping of blg1(beak like grain 1), a Rice Mutant with Abnormal Palea and Lemma Development
Received date: 2022-05-09
Revised date: 2022-12-02
Online published: 2023-05-16
【Objective】 The development of rice floral organs is very important for the successful reproduction and yield of rice. Genetic mapping of genes related to floret organ development can improve the regulatory network of rice floret development, which has theoretical significance for rice breeding.【Method】A mutant blg1 with abnormal palea and lemma was isolated and identified from breeding materials. The floret morphology of blg1 and control line was observed. The mutant was hybridized with Dular to form an F2 population, and then genetic analysis, fine mapping and candidate gene analysis were carried out.【Result】Phenotypic observation showed that the palea and lemma in blg1 could not closed normally and curved severely with smaller grains. Genetic analysis showed that the mutant phenotype was controlled by a pair of recessive nuclear genes. Through fine mapping, the objective gene was located between markers InD3 and InD5 at the end of the long arm on chromosome 5, with a physical distance of 50 kb, including eight Open Reading Frames. By candidate gene analysis, LOC_Os05g48760 may be a candidate gene for BLG1 gene.【Conclusion】BLG1 is a new gene controlling the development of palea and lemma in rice.
Key words： rice; palea and lemma; fine mapping
SHEN Yumin, CHEN Mingliang, XIONG Huanjin, XIONG Wentao, WU Xiaoyan, XIAO Yeqing . Phenotypic Analysis and Fine Mapping of blg1(beak like grain 1), a Rice Mutant with Abnormal Palea and Lemma Development[J]. Chinese Journal OF Rice Science, 2023 , 37(3) : 225 -232 . DOI: 10.16819/j.1001-7216.2023.220506
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