Chinese Journal OF Rice Science >
Fine-mapping of SegD8 Loci for Rice Hybrid Segregation Distortion
Received date: 2022-02-02
Revised date: 2022-04-01
Online published: 2023-01-10
【Objective】 This study aims to fine-map and clone genes related to segregation distortion(SD) by using a rice introgressive line population, and thus to explore the molecular mechanism underlying the segregation distortion in rice. 【Method】 A set of introgressive line population was constructed by using an indica/japonica combination Huazhan/Koliya. The gene loci were mapped with different genotype separation ratios of offspring as phenotypes. 【Result】 We identified a segment on chromosome 8 that displayed obvious deviated segregation ratio from Mendelian's separation law. Further analysis revealed that the SD was controlled by two loci on chromosome 8, which were termed as SegD8A and SegD8B, respectively. SegD8A was a segregation locus, but its segregation was regulated by SegD8B. When both SegD8A and SegD8B were heterozygous, the ratio of SegD8AHH and SegD8AKK in progeny was close to 1:4. When SegD8B was homozygous Huazhan genotype, the ratio of SegD8AHH and SegD8AKK in progenies was close to 1:1. When SegD8B was homozygous Koliya genotype, SegD8AHH almost did not appear in its progeny. The SegD8A gene was mapped to a 46.5 kb region and the SegD8B gene was mapped to an 1160.2 kb region using the separation ratio of the progeny. 【Conclusion】 The results lay a foundation for further cloning of these two genes and elucidating the partial separation phenomenon.
YUAN Yang, AO Hejun, ZHOU Zhonghua, YING Jiezheng, ZHANG Jian, NI Shen . Fine-mapping of SegD8 Loci for Rice Hybrid Segregation Distortion[J]. Chinese Journal OF Rice Science, 2023 , 37(1) : 37 -44 . DOI: 10.16819/j.1001-7216.2023.220203
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