Chinese Journal OF Rice Science >
Isolation and Identification of Entomopathogenic Nematode N-Yz1 from Chilo Suppressalis and Its Infection Characteristics
Received date: 2021-12-02
Revised date: 2022-04-02
Online published: 2022-11-10
【Objective】 The rice stem borer [Chilo suppressalis (walker)] is an important pest to rice that threatens rice growth. The fact that it bores into rice stems adds to the difficulties of control. This study focuses on the isolation of the entomopathogenic nematodes of C. suppressalis and analysis of the infection of the pest to provide new strategies and methods for the control of boring pests on rice. 【Method】 We obtained and identified entomopathogenic nematodes from the overwintering generation C. suppressalis in the rice stubbles through tissue isolation. Then, the lethal effect and infecting characters of the isolated entomopathogenic nematodes against the C. suppressalis were analyzed by inoculation into the larvae of C. suppressalis. 【Result】 The wild-infected entomopathogenic nematode named N-Yz1 was isolated from dead larvae of C. suppressalis, then the isolated nematodes were inoculated into the 4th instar larvae of C. suppressalis, leading to their infection and death, with mass nematodes emerged in the dead larvae. Based on the morphological features and analysis of the sequences of rDNA ITS, the strain of N-Yz1 was identified in the genus of Steinernema, which shared the homology of 99.89% with that of the species of Steinernema carpocapsae. The nematode N-Yz1 had highly infectious and lethal effects on different instar larvae of C. suppressalis. Inoculation with a nematode dose of 40 IJs per larva resulted in rapid lethality and high mortality of the juvenile larvae (the second and third instar), with infected lethal individuals appearing within 24 h after inoculation and the death of all larvae 48 h later. For the elder larvae (the fourth to sixth instar), the killing speed was relatively slow, with peak mortality occurring 48 h after nematode inoculation. We also found that positive correlation between inoculum dosage of N-Yz1 and the mortality rate of C. suppressalis. The mortality rates of the 4th instar C. suppresalis were more than 90% when the inoculation dose exceeded 30 IJs per larva. The results also showed that N-Yz1 could forage C. suppressalis larvae within the distance of 30 cm, and kill the larvae through immigrating and diffusion. 【Conclusion】 The isolated entomopathogenic nematode N-Yz1, a strain of Steinernema, has strong infective and lethal effect, and potential applications for the control of the rice stem borer, C. suppressalis.
LIU Qin, XIA Yang, HAN Guangjie, LI Chuanming, LU Yurong, HUANG Lixin, QI Jianhang, XU Jian . Isolation and Identification of Entomopathogenic Nematode N-Yz1 from Chilo Suppressalis and Its Infection Characteristics[J]. Chinese Journal OF Rice Science, 2022 , 36(6) : 639 -646 . DOI: 10.16819/j.1001-7216.2022.211202
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