Chinese Journal OF Rice Science >
Control Effect of St-79 (Streptomyces thioluteus) on Rice Bacterial Blight and Its Growth-promoting Effect
Received date: 2021-11-02
Revised date: 2022-04-05
Online published: 2022-11-10
【Objective】 Rice bacterial blight (RBB) is one of the main bacterial diseases on rice, which seriously hinders rice production. The purpose of this study is to obtain an effective actinomycete against RBB and explore its biocontrol and growth-promoting effects, so as to lay a scientific basis for the development and utilization of biocontrol bacteria.【Method】 Actinomycetes against Xanthomonas oryzae pv. oryzae (Xoo) were screened by gradient dilution coating method, co-culture method and Oxford cup method. They were classified and identified by morphological traits, physiological and biochemical reactions, 16S rDNA sequences and phylogenetic-tree analysis. The antimicrobial spectrum of the target strain was analyzed by co-culture method. Antibacterial effect of the target strain was studied by 96-well microdilution method, scanning electron microscope and microbial adhesion hydrocarbon method; The promoting effect of fermentation liquid on rice growth was explored by soaking seeds and spraying soil. The control effect of fermentation liquid of the target strain on rice bacterial blight was studied in a pot experiment.【Result】 A total of 140 actinomycetes strains were isolated from soil in different habitats, and one actinomycete strain St-79 with the strongest antagonistic activity against Xoo was finally screened. The diameter of inhibition zone of fermentation liquid was 64 mm, and it was identified as Streptomyces thioluteus. The strain St-79 had antagonistic effects against Xanthomonas oryzae pv. oryzicola, Xanthomonas axonopodis pv. glycines, Xanthomonas campestris pv. campestris and Pseudomonas syringae pv. tomato. The ethyl acetate crude extract had good inhibitory effect on the growth of Xoo. The minimum inhibitory concentration of crude extract on Xoo was 8 μg/mL, and the minimum bactericidal concentration was 32 μg/mL. The observation results under a scanning electron microscopy showed that the crude extract had a strong damage effect on Xoo cells. Crude extracts can also change the permeability of Xoo cells and reduce the hydrophobicity of Xoo cells. The results of growth-promoting test showed that the fermentation liquid of St-79 could promote the growth of rice seedlings. The pot experiment results showed that the fermentation liquid of St-79 had good control effect on RBB. The relative control efficacies on Nipponbare, Yongyou 15 and Yongyou 1540 was 65.95%－87.23%, and the lesion inhibition rate were 69.85%－95.8%. The prevention effect was better than the post-disease treatment.【Conclusion】 An effective actinomycete against RBB was obtained, which had obvious inhibitory effect on RBB and could promote the germination and seedling growth of rice.
MA Jingjing, PAN Yanyan, YANG Sunyuyue, WANG Jiaqi, JIANG Donghua . Control Effect of St-79 (Streptomyces thioluteus) on Rice Bacterial Blight and Its Growth-promoting Effect[J]. Chinese Journal OF Rice Science, 2022 , 36(6) : 623 -638 . DOI: 10.16819/j.1001-7216.2022.211102
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