Chinese Journal OF Rice Science >
Improvement of Herbicide Resistance in Rice by Using CRISPR/Cas9 System
Received date: 2021-10-13
Revised date: 2021-12-07
Online published: 2022-09-09
【Objective】 It is of great significance to create herbicide-resistant rice varieties. The acetyllactic acid synthase (ALS) gene was edited to create rice varieties with excellent herbicide resistance using non-herbicide-resistant japonica rice varieties with good quality as materials. 【Methods】Using CRISPR/Cas9 technology, the single-base mutant vector pH-NCas9-PBA-ALS was constructed with acyllactic acid synthase (ALS) as the target gene. Songjing 22, Longjing 46 and Suijing 18 were used as transformation materials to obtain transgenic plants through Agrobacterium-mediated genetic transformation. Genotypes and phenotypes were identified by sequencing the mutation sites of transgenic plants and herbicide spraying test. 【Results】 Ten ALSS627N mutants, one ALSS627N and 1884G-A(the 628th amino acid unchanged mutant), and one ALSS627N/G628E mutant were obtained by molecular level detection. Compared with the wild type, the three types of mutants had stronger herbicide resistance. 【Conclusion】 Using CRISPR/Cas9 gene editing technology, we obtained homozygous mutant lines which are genetically stable and herbicide-resistant, providing basic materials for herbicide-resistant rice breeding.
Key words： rice; ALS; herbicide resistance; imazethapyr; CRISPR/Cas9; gene editing
YIN Liying, ZHANG Yuanye, LI Rongtian, HE Mingliang, WANG Fangquan, XU Yang, LIU Xinxin, PAN Tingting, TIAN Xiaojie, BU Qingyun, LI Xiufeng . Improvement of Herbicide Resistance in Rice by Using CRISPR/Cas9 System[J]. Chinese Journal OF Rice Science, 2022 , 36(5) : 459 -466 . DOI: 10.16819/j.1001-7216.2022.211004
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