Chinese Journal OF Rice Science >
Interactive Effects of HCO3− and Irrigation Methods on Iron Uptake and Utilization in Rice
Received date: 2021-09-11
Revised date: 2022-05-11
Online published: 2022-07-12
【Objective】Drip-irrigated rice with plastic film mulch (DI-PFM) is a rice cultivation technique with both high yield and water-saving potential. However, DI-PFM rice on calcareous soil often exhibits Fe-chlorosis symptoms. Previous studies found that the inhibitory intensity of HCO3− on Fe uptake of DI-PFM rice was weaker than that of flooding irrigation rice, but the underlying mechanism remains unclear. The effects of HCO3− on soil DTPA-Fe concentration, xylem sap pH, Fe absorption and distribution of two kinds of irrigated rice were studied. 【Methods】The experiment included two irrigation methods [flooding irrigation (FI) and DI-PFM] and four HCO3− concentrations (0, 2, 10 and 40 mmol/L HCO3−, were expressed as BC-0, BC-2, BC-10 and BC-40, respectively) of irrigation water. 【Results】The concentration of soil DTPA-Fe in DI-PFM was significantly lower than that in FI. With the increase of HCO3− concentration, soil pH increased, while soil DTPA-Fe concentration decreased. Xylem sap pH of rice increased with the increase of HCO3− concentration in irrigation water, and the xylem sap pH of FI rice was significantly higher than that of DI-PFM. With the increase of HCO3− concentration, the apoplast Fe concentration in rice leaves and roots decreased at low HCO3− level and increased at high HCO3− level, and peaked under BC-40 treatment. The leaf active Fe concentration, symplastic Fe concentration, rice biomass and total Fe accumulation of two kinds of irrigated rice decreased with the increase of HCO3− concentration, but the decrease of these parameters under DI-PFM treatment were less than that of FI. The concentration ratio of symplastic Fe to apoplast Fe in rice roots and leaves decreased with the increase of HCO3− concentration, and the concentration ratio of symplastic Fe to apoplast Fe in rice roots and leaves in FI was higher than that of DI-PFM. 【Conclusion】The increased pH in xylem sap of rice caused by HCO3− affected the absorption and utilization of Fe in rice, but HCO3− was not the main factor leading to Fe deficiency in DI-PFM rice. The main reason for Fe deficiency of DI-PFM rice on calcareous soil may still be due to the low availability of soil Fe.
Key words： apoplastic Fe; HCO3−; Fe uptake and utilization; symplastic Fe; xylem sap
ZHANG Xinjiang, WANG Xiangbin, LIU Linghui, WEI Changzhou . Interactive Effects of HCO3− and Irrigation Methods on Iron Uptake and Utilization in Rice[J]. Chinese Journal OF Rice Science, 2022 , 36(4) : 419 -427 . DOI: 10.16819/j.1001-7216.2022.210904
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