Chinese Journal OF Rice Science >
Effects of Moisture Content on Root Vigor and the Expression of Aquaporin-related Genes in Rice Seedlings Under Low Temperature Stress
Received date: 2021-08-09
Revised date: 2021-10-02
Online published: 2022-07-12
Abstract：【Objective】The purpose of this study is to explore the effects of moisture content or humidity on root vigor and water transport of rice seedlings under low temperature stress. 【Method】Two rice cultivars (Jiaxian 7 and Fu 8329) with different chilling tolerance were used as materials. Experiment I (humidity 30%, 60% and 90%) and experiment Ⅱ (quick rewarming experiment) were carried out. 【Result】Compared with lower humidity (30%), higher humidity (60% and 90%) could effectively increase the root vigor, ensure the survival rate of rice seedlings and alleviate the chilling injury. The expression level of OsPIP2;5 and OsPIP2;6 were closely related to root vigor, which had a significant regulatory effect on cold tolerance of rice. Compared with normal rewarming treatment (CK), the water content and root vigor of rice seedlings were decreased after quick rewarming treatment. The survival rate in CK was significantly higher than in the quick rewarming experiment. 【Conclusion】 Low temperature stress affects the growth and development of rice seedlings. After chilling injury, high humidity can alleviate the damage and ensure the rice survival rate. Humidity environment after low temperature stress significantly influenced the water content, root vigor and the expression levels of genes related to aquaporin. The expression levels of OsPIP2;5 and OsPIP2;6 were positively correlated with root vigor, and played an important role in resistance to cold injury of rice seedlings. Therefore, compared with large-scale irrigation in the field after low temperature stress, the slow soil temperature rise had less effect on root vigor and can help rice seedlings to restore their life activities.
Key words： rice; low temperature stress; gene expression; root vigor; aquaporin; moisture content
HUANG Qina, JIANG Su, WANG Limin, ZHANG Yan, YU Linfei, LI Chunfu, DING Liqun, SHAO Guosheng . Effects of Moisture Content on Root Vigor and the Expression of Aquaporin-related Genes in Rice Seedlings Under Low Temperature Stress[J]. Chinese Journal OF Rice Science, 2022 , 36(4) : 367 -376 . DOI: 10.16819/j.1001-7216.2022.210805
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